The food-associated resistome is shaped by processing and production environments

[EN] Food production systems may act as transmission routes for antimicrobial-resistant (AMR) bacteria and AMR genes (AMRGs) to humans. However, the food resistome remains poorly characterized. Here 1,780 raw-material (milk, brine, fresh meat and so on), end-product (cheese, fish, meat products and...

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Autores: Quijada, Narciso M., Cobo Díaz, José Francisco, Valentino, Vincenzo, Barcenilla Canduela, Coral, De Filippis, Francesca, Cabrera Rubio, Raúl, Carlino, Niccolò, Pinto, Federica, Dzieciol, Monika, Calvete Torre, Inés, Sabater, Carlos, Rubino, Francesco, Knobloch, Stephen, Skírnisdóttir, Sigurlaug, Ruiz, Lorena, López Fernández, María Mercedes, Prieto Maradona, Miguel, Marteinsson, Viggó Thór, Margolles, Abelardo, Segata, Nicola, Cotter, Paul D., Wagner, Martin, Ercolini, Danilo, Álvarez Ordóñez, Avelino
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Recursos:Universidad de León
Repositorio:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:buleria.unileon.es:10612/26275
Acesso em linha:https://www.nature.com/articles/s41564-025-02059-8
https://hdl.handle.net/10612/26275
Access Level:acceso abierto
Palavra-chave:Tecnología de los alimentos
Food-associated resistome
Antimicrobial-resistant (AMR)
Bacteria and AMR genes (AMRGs)
Food production systems
3309.90 Microbiología de Alimentos
3309 Tecnología de Los Alimentos
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spelling The food-associated resistome is shaped by processing and production environmentsQuijada, Narciso M.Cobo Díaz, José FranciscoValentino, VincenzoBarcenilla Canduela, CoralDe Filippis, FrancescaCabrera Rubio, RaúlCarlino, NiccolòPinto, FedericaDzieciol, MonikaCalvete Torre, InésSabater, CarlosRubino, FrancescoKnobloch, StephenSkírnisdóttir, SigurlaugRuiz, LorenaLópez Fernández, María MercedesPrieto Maradona, MiguelMarteinsson, Viggó ThórMargolles, AbelardoSegata, NicolaCotter, Paul D.Wagner, MartinErcolini, DaniloÁlvarez Ordóñez, AvelinoTecnología de los alimentosFood-associated resistomeAntimicrobial-resistant (AMR)Bacteria and AMR genes (AMRGs)Food production systems3309.90 Microbiología de Alimentos3309 Tecnología de Los Alimentos[EN] Food production systems may act as transmission routes for antimicrobial-resistant (AMR) bacteria and AMR genes (AMRGs) to humans. However, the food resistome remains poorly characterized. Here 1,780 raw-material (milk, brine, fresh meat and so on), end-product (cheese, fish, meat products and vegetables) and surface (processing, cooling, smoking, ripening and packing rooms) samples from 113 food processing facilities were subjected to whole-metagenome sequencing. Assembly-free analyses demonstrated that >70% of all known AMRGs, including many predicted to confer resistance to critically important antibiotics, circulate throughout food production chains, with those conferring resistance to tetracyclines, β-lactams, aminoglycosides and macrolides being the most abundant overall. An assembly-based analysis highlighted that bacteria from the ESKAPEE group, together with Staphylococcus equorum and Acinetobacter johnsonii, were the main AMRG carriers. Further evaluation demonstrated that ~40% of the AMRGs were associated with mobile genetic elements, mainly plasmids. These findings will help guide the appropriate use of biocides and other antimicrobials in food production settings when designing efficient antimicrobial stewardship policiesSIThis work was funded by the European Commission under the European Union’s Horizon 2020 research and innovation programme under grant agreement no. 818368 (MASTER). N.M.Q. is currently funded under the Generation D initiative, promoted by Red.es, an organization attached to the Ministry for Digital Transformation and the Civil Service, for the attraction and retention of talent through grants and training contracts, financed by the Recovery, Transformation and Resilience Plan through the European Union’s Next Generation funds (ref. MMT24-IBFG-01). C.B. is grateful to Junta de Castilla y León and the European Social Fund for awarding her a predoctoral grant (grant no. BOCYL-D-07072020-6). The COMET-K1 competence centre FFoQSI (number 881882) is funded by the Austrian federal ministries BMK, BMDW and the Austrian provinces Lower Austria, Upper Austria and Vienna within the scope of COMET - Competence Centers for Excellent Technologies. The program COMET is handled by the Austrian Research Promotion Agency, FFGNature ResearchTecnologia de los AlimentosFacultad de Veterinaria2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://www.nature.com/articles/s41564-025-02059-8https://hdl.handle.net/10612/26275reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad de LeónIngléshttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/262752026-06-24T12:43:27Z
dc.title.none.fl_str_mv The food-associated resistome is shaped by processing and production environments
title The food-associated resistome is shaped by processing and production environments
spellingShingle The food-associated resistome is shaped by processing and production environments
Quijada, Narciso M.
Tecnología de los alimentos
Food-associated resistome
Antimicrobial-resistant (AMR)
Bacteria and AMR genes (AMRGs)
Food production systems
3309.90 Microbiología de Alimentos
3309 Tecnología de Los Alimentos
title_short The food-associated resistome is shaped by processing and production environments
title_full The food-associated resistome is shaped by processing and production environments
title_fullStr The food-associated resistome is shaped by processing and production environments
title_full_unstemmed The food-associated resistome is shaped by processing and production environments
title_sort The food-associated resistome is shaped by processing and production environments
dc.creator.none.fl_str_mv Quijada, Narciso M.
Cobo Díaz, José Francisco
Valentino, Vincenzo
Barcenilla Canduela, Coral
De Filippis, Francesca
Cabrera Rubio, Raúl
Carlino, Niccolò
Pinto, Federica
Dzieciol, Monika
Calvete Torre, Inés
Sabater, Carlos
Rubino, Francesco
Knobloch, Stephen
Skírnisdóttir, Sigurlaug
Ruiz, Lorena
López Fernández, María Mercedes
Prieto Maradona, Miguel
Marteinsson, Viggó Thór
Margolles, Abelardo
Segata, Nicola
Cotter, Paul D.
Wagner, Martin
Ercolini, Danilo
Álvarez Ordóñez, Avelino
author Quijada, Narciso M.
author_facet Quijada, Narciso M.
Cobo Díaz, José Francisco
Valentino, Vincenzo
Barcenilla Canduela, Coral
De Filippis, Francesca
Cabrera Rubio, Raúl
Carlino, Niccolò
Pinto, Federica
Dzieciol, Monika
Calvete Torre, Inés
Sabater, Carlos
Rubino, Francesco
Knobloch, Stephen
Skírnisdóttir, Sigurlaug
Ruiz, Lorena
López Fernández, María Mercedes
Prieto Maradona, Miguel
Marteinsson, Viggó Thór
Margolles, Abelardo
Segata, Nicola
Cotter, Paul D.
Wagner, Martin
Ercolini, Danilo
Álvarez Ordóñez, Avelino
author_role author
author2 Cobo Díaz, José Francisco
Valentino, Vincenzo
Barcenilla Canduela, Coral
De Filippis, Francesca
Cabrera Rubio, Raúl
Carlino, Niccolò
Pinto, Federica
Dzieciol, Monika
Calvete Torre, Inés
Sabater, Carlos
Rubino, Francesco
Knobloch, Stephen
Skírnisdóttir, Sigurlaug
Ruiz, Lorena
López Fernández, María Mercedes
Prieto Maradona, Miguel
Marteinsson, Viggó Thór
Margolles, Abelardo
Segata, Nicola
Cotter, Paul D.
Wagner, Martin
Ercolini, Danilo
Álvarez Ordóñez, Avelino
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Tecnologia de los Alimentos
Facultad de Veterinaria
dc.subject.none.fl_str_mv Tecnología de los alimentos
Food-associated resistome
Antimicrobial-resistant (AMR)
Bacteria and AMR genes (AMRGs)
Food production systems
3309.90 Microbiología de Alimentos
3309 Tecnología de Los Alimentos
topic Tecnología de los alimentos
Food-associated resistome
Antimicrobial-resistant (AMR)
Bacteria and AMR genes (AMRGs)
Food production systems
3309.90 Microbiología de Alimentos
3309 Tecnología de Los Alimentos
description [EN] Food production systems may act as transmission routes for antimicrobial-resistant (AMR) bacteria and AMR genes (AMRGs) to humans. However, the food resistome remains poorly characterized. Here 1,780 raw-material (milk, brine, fresh meat and so on), end-product (cheese, fish, meat products and vegetables) and surface (processing, cooling, smoking, ripening and packing rooms) samples from 113 food processing facilities were subjected to whole-metagenome sequencing. Assembly-free analyses demonstrated that >70% of all known AMRGs, including many predicted to confer resistance to critically important antibiotics, circulate throughout food production chains, with those conferring resistance to tetracyclines, β-lactams, aminoglycosides and macrolides being the most abundant overall. An assembly-based analysis highlighted that bacteria from the ESKAPEE group, together with Staphylococcus equorum and Acinetobacter johnsonii, were the main AMRG carriers. Further evaluation demonstrated that ~40% of the AMRGs were associated with mobile genetic elements, mainly plasmids. These findings will help guide the appropriate use of biocides and other antimicrobials in food production settings when designing efficient antimicrobial stewardship policies
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.nature.com/articles/s41564-025-02059-8
https://hdl.handle.net/10612/26275
url https://www.nature.com/articles/s41564-025-02059-8
https://hdl.handle.net/10612/26275
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Nature Research
publisher.none.fl_str_mv Nature Research
dc.source.none.fl_str_mv reponame:BULERIA. Repositorio Institucional de la Universidad de León
instname:Universidad de León
instname_str Universidad de León
reponame_str BULERIA. Repositorio Institucional de la Universidad de León
collection BULERIA. Repositorio Institucional de la Universidad de León
repository.name.fl_str_mv
repository.mail.fl_str_mv
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