Singlet oxygen in antimicrobial photodynamic therapy: photosensitizer-dependent production and decay in E. coli

Several families of photosensitizers are currently being scrutinized for antimicrobial photodynamic therapy applications. Differences in physical and photochemical properties can lead to different localization patterns as well as differences in singlet oxygen production and decay when the photosensi...

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Detalles Bibliográficos
Autores: Nonell, Santi, Agut, Montserrat, Ragàs Amalrich, Xavier, He, Xin, Roxo-Rosa, Mónica, Rocha Gonsalves, António, Serra, Arménio C.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:20.500.14342/994
Acceso en línea:http://hdl.handle.net/20.500.14342/994
https://doi.org/10.3390/molecules18032712
Access Level:acceso abierto
Palabra clave:Fotoquimioteràpia
Anàlisi espectral
Escheríchia coli
Antimicrobial photodynamic therapy
Cationic photosensitizers
E. coli
Kinetics
Photodynamic inactivation
Singlet oxygen
Time-resolved near-IR spectroscopy
577
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spelling Singlet oxygen in antimicrobial photodynamic therapy: photosensitizer-dependent production and decay in E. coliNonell, SantiAgut, MontserratRagàs Amalrich, XavierHe, XinRoxo-Rosa, MónicaRocha Gonsalves, AntónioSerra, Arménio C.FotoquimioteràpiaAnàlisi espectralEscheríchia coliAntimicrobial photodynamic therapyCationic photosensitizersE. coliKineticsPhotodynamic inactivationSinglet oxygenTime-resolved near-IR spectroscopy577Several families of photosensitizers are currently being scrutinized for antimicrobial photodynamic therapy applications. Differences in physical and photochemical properties can lead to different localization patterns as well as differences in singlet oxygen production and decay when the photosensitizers are taken up by bacterial cells. We have examined the production and fate of singlet oxygen in Escherichia coli upon photosensitization with three structurally-different cationic photosensitizers, namely New Methylene Blue N (NMB), a member of the phenothiazine family, ACS268, a hydrophobic porphyrin with a single cationic alkyl chain, and zinc(II)-tetramethyltetrapyridinoporphyrazinium salt, a phthalocyanine-like photosensitizer with four positive charges on the macrocycle core. The kinetics of singlet oxygen production and decay indicate different localization for the three photosensitizers, whereby NMB appears to localize in an aqueous-like microenvironment, whereas ACS268 localizes in an oxygen-shielded site, highly reactive towards singlet oxygen. The tetracationic zinc(II) tetrapyridinoporphyrazine is extensively aggregated in the bacteria and fails to produce any detectable singlet oxygen.MDPIUniversitat Ramon Llull. IQS2013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion14 p.http://hdl.handle.net/20.500.14342/994https://doi.org/10.3390/molecules18032712reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésMolecules. Vol.18, n.3 (2013), p.2712-2725info:eu-repo/grantAgreement/MINECO/PN I+D/ CTQ2007-67763-C03-01info:eu-repo/grantAgreement/MINECO/PN I+D/CTQ2010-20870-C03-01Attribution 4.0 International© L'autor/ahttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:20.500.14342/9942026-05-29T05:05:01Z
dc.title.none.fl_str_mv Singlet oxygen in antimicrobial photodynamic therapy: photosensitizer-dependent production and decay in E. coli
title Singlet oxygen in antimicrobial photodynamic therapy: photosensitizer-dependent production and decay in E. coli
spellingShingle Singlet oxygen in antimicrobial photodynamic therapy: photosensitizer-dependent production and decay in E. coli
Nonell, Santi
Fotoquimioteràpia
Anàlisi espectral
Escheríchia coli
Antimicrobial photodynamic therapy
Cationic photosensitizers
E. coli
Kinetics
Photodynamic inactivation
Singlet oxygen
Time-resolved near-IR spectroscopy
577
title_short Singlet oxygen in antimicrobial photodynamic therapy: photosensitizer-dependent production and decay in E. coli
title_full Singlet oxygen in antimicrobial photodynamic therapy: photosensitizer-dependent production and decay in E. coli
title_fullStr Singlet oxygen in antimicrobial photodynamic therapy: photosensitizer-dependent production and decay in E. coli
title_full_unstemmed Singlet oxygen in antimicrobial photodynamic therapy: photosensitizer-dependent production and decay in E. coli
title_sort Singlet oxygen in antimicrobial photodynamic therapy: photosensitizer-dependent production and decay in E. coli
dc.creator.none.fl_str_mv Nonell, Santi
Agut, Montserrat
Ragàs Amalrich, Xavier
He, Xin
Roxo-Rosa, Mónica
Rocha Gonsalves, António
Serra, Arménio C.
author Nonell, Santi
author_facet Nonell, Santi
Agut, Montserrat
Ragàs Amalrich, Xavier
He, Xin
Roxo-Rosa, Mónica
Rocha Gonsalves, António
Serra, Arménio C.
author_role author
author2 Agut, Montserrat
Ragàs Amalrich, Xavier
He, Xin
Roxo-Rosa, Mónica
Rocha Gonsalves, António
Serra, Arménio C.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universitat Ramon Llull. IQS
dc.subject.none.fl_str_mv Fotoquimioteràpia
Anàlisi espectral
Escheríchia coli
Antimicrobial photodynamic therapy
Cationic photosensitizers
E. coli
Kinetics
Photodynamic inactivation
Singlet oxygen
Time-resolved near-IR spectroscopy
577
topic Fotoquimioteràpia
Anàlisi espectral
Escheríchia coli
Antimicrobial photodynamic therapy
Cationic photosensitizers
E. coli
Kinetics
Photodynamic inactivation
Singlet oxygen
Time-resolved near-IR spectroscopy
577
description Several families of photosensitizers are currently being scrutinized for antimicrobial photodynamic therapy applications. Differences in physical and photochemical properties can lead to different localization patterns as well as differences in singlet oxygen production and decay when the photosensitizers are taken up by bacterial cells. We have examined the production and fate of singlet oxygen in Escherichia coli upon photosensitization with three structurally-different cationic photosensitizers, namely New Methylene Blue N (NMB), a member of the phenothiazine family, ACS268, a hydrophobic porphyrin with a single cationic alkyl chain, and zinc(II)-tetramethyltetrapyridinoporphyrazinium salt, a phthalocyanine-like photosensitizer with four positive charges on the macrocycle core. The kinetics of singlet oxygen production and decay indicate different localization for the three photosensitizers, whereby NMB appears to localize in an aqueous-like microenvironment, whereas ACS268 localizes in an oxygen-shielded site, highly reactive towards singlet oxygen. The tetracationic zinc(II) tetrapyridinoporphyrazine is extensively aggregated in the bacteria and fails to produce any detectable singlet oxygen.
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.14342/994
https://doi.org/10.3390/molecules18032712
url http://hdl.handle.net/20.500.14342/994
https://doi.org/10.3390/molecules18032712
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Molecules. Vol.18, n.3 (2013), p.2712-2725
info:eu-repo/grantAgreement/MINECO/PN I+D/ CTQ2007-67763-C03-01
info:eu-repo/grantAgreement/MINECO/PN I+D/CTQ2010-20870-C03-01
dc.rights.none.fl_str_mv Attribution 4.0 International
© L'autor/a
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
© L'autor/a
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 14 p.
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869422779219050496
score 15,812429

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