Optimization of a method to extract polysaccharides from white grape pomace by-products

The aim of this paper is to optimize a method to recover polysaccharides from white grape pomace (non-fermented), the main waste by-product of the food industry. Different conditions are tested and the polysaccharides extracted are analyzed by high performance size exclusion chromatography with refr...

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Authors: Canalejo, Diego, Guadalupe, Zenaida, Martínez Lapuente, Leticia, Ayestarán, Belén, Pérez-Magariño, Silvia
Format: article
Status:Published version
Publication Date:2021
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/262108
Online Access:http://hdl.handle.net/10261/262108
Access Level:Open access
Keyword:Carbohydrates
Grape waste
Isolation
Polysaccharides rich in arabinose and galactose (PRAG)
Rhamnogalacturonan type II (RG-II)
Homogalacturonans (HG)
Glucosyl polysaccharides (GP)
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spelling Optimization of a method to extract polysaccharides from white grape pomace by-productsCanalejo, DiegoGuadalupe, ZenaidaMartínez Lapuente, LeticiaAyestarán, BelénPérez-Magariño, SilviaCarbohydratesGrape wasteIsolationPolysaccharides rich in arabinose and galactose (PRAG)Rhamnogalacturonan type II (RG-II)Homogalacturonans (HG)Glucosyl polysaccharides (GP)The aim of this paper is to optimize a method to recover polysaccharides from white grape pomace (non-fermented), the main waste by-product of the food industry. Different conditions are tested and the polysaccharides extracted are analyzed by high performance size exclusion chromatography with refractive index detector (HPSEC-RID) and gas chromatography with mass detector (GC-MS). The extraction solvent did not show a significant effect on the polysaccharide extraction, acid pH yielded to higher efficiencies, and longer extraction times extracted more smaller polysaccharides (≤5.4 kg mol). The highest efficiencies were obtained with both solvents at pH 1 and 1:4 solid to liquid ratio. The optimum conditions selected (TA as solvent of extraction, 2.5 g L solvent concentration, pH = 1, 1:4 solid to liquid ratio, and 18 h of extraction time) allow the extraction of polysaccharides rich in arabinose and galactose, rhamnogalacturonans, homogalacturonans and glucosyl polysaccharides, under efficient and food-safe conditions.The authors would like to thank the Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) and the Ministerio de Ciencia e Innovación for the funding provided for this study through the project RTA2017-00005-C02-02.ElsevierCSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)Ministerio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2022202220212022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/262108reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI//RTA2017-00005-C02-02http://dx.doi.org/10.1016/j.foodchem.2021.130445Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2621082026-05-22T06:33:51Z
dc.title.none.fl_str_mv Optimization of a method to extract polysaccharides from white grape pomace by-products
title Optimization of a method to extract polysaccharides from white grape pomace by-products
spellingShingle Optimization of a method to extract polysaccharides from white grape pomace by-products
Canalejo, Diego
Carbohydrates
Grape waste
Isolation
Polysaccharides rich in arabinose and galactose (PRAG)
Rhamnogalacturonan type II (RG-II)
Homogalacturonans (HG)
Glucosyl polysaccharides (GP)
title_short Optimization of a method to extract polysaccharides from white grape pomace by-products
title_full Optimization of a method to extract polysaccharides from white grape pomace by-products
title_fullStr Optimization of a method to extract polysaccharides from white grape pomace by-products
title_full_unstemmed Optimization of a method to extract polysaccharides from white grape pomace by-products
title_sort Optimization of a method to extract polysaccharides from white grape pomace by-products
dc.creator.none.fl_str_mv Canalejo, Diego
Guadalupe, Zenaida
Martínez Lapuente, Leticia
Ayestarán, Belén
Pérez-Magariño, Silvia
author Canalejo, Diego
author_facet Canalejo, Diego
Guadalupe, Zenaida
Martínez Lapuente, Leticia
Ayestarán, Belén
Pérez-Magariño, Silvia
author_role author
author2 Guadalupe, Zenaida
Martínez Lapuente, Leticia
Ayestarán, Belén
Pérez-Magariño, Silvia
author2_role author
author
author
author
dc.contributor.none.fl_str_mv CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)
Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Carbohydrates
Grape waste
Isolation
Polysaccharides rich in arabinose and galactose (PRAG)
Rhamnogalacturonan type II (RG-II)
Homogalacturonans (HG)
Glucosyl polysaccharides (GP)
topic Carbohydrates
Grape waste
Isolation
Polysaccharides rich in arabinose and galactose (PRAG)
Rhamnogalacturonan type II (RG-II)
Homogalacturonans (HG)
Glucosyl polysaccharides (GP)
description The aim of this paper is to optimize a method to recover polysaccharides from white grape pomace (non-fermented), the main waste by-product of the food industry. Different conditions are tested and the polysaccharides extracted are analyzed by high performance size exclusion chromatography with refractive index detector (HPSEC-RID) and gas chromatography with mass detector (GC-MS). The extraction solvent did not show a significant effect on the polysaccharide extraction, acid pH yielded to higher efficiencies, and longer extraction times extracted more smaller polysaccharides (≤5.4 kg mol). The highest efficiencies were obtained with both solvents at pH 1 and 1:4 solid to liquid ratio. The optimum conditions selected (TA as solvent of extraction, 2.5 g L solvent concentration, pH = 1, 1:4 solid to liquid ratio, and 18 h of extraction time) allow the extraction of polysaccharides rich in arabinose and galactose, rhamnogalacturonans, homogalacturonans and glucosyl polysaccharides, under efficient and food-safe conditions.
publishDate 2021
dc.date.none.fl_str_mv 2021
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/262108
url http://hdl.handle.net/10261/262108
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI//RTA2017-00005-C02-02
http://dx.doi.org/10.1016/j.foodchem.2021.130445
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869422621102178304
score 15,812429

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