Magnetic particle-based enzyme assays and immunoassays for microcystins: from colorimetric to electrochemical detection

In this work, magnetic particles (MPs) are used as supports for the immobilization of biorecognition molecules for the detection of microcystins (MCs). In one approach, a recombinant protein phosphatase 1 (PP1) has been conjugated to MPs via coordination chemistry, and MC-LR detection has been based...

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Autores: Reverté, Laia, Garibo, Diana, Flores, Cintia, Diogène, Jorge, Caixach, Josep, Campàs, Mònica
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2013
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/413894
Acceso en línea:http://hdl.handle.net/10261/413894
https://api.elsevier.com/content/abstract/scopus_id/84871752751
Access Level:acceso abierto
Palabra clave:Magnetic particles (MPs)
LC-MS/MS
http://metadata.un.org/sdg/3
http://metadata.un.org/sdg/9
Ensure healthy lives and promote well-being for all at all ages
Build resilient infrastructure, promote inclusive and sustainable industrialization and foster innovation
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spelling Magnetic particle-based enzyme assays and immunoassays for microcystins: from colorimetric to electrochemical detectionReverté, LaiaGaribo, DianaFlores, CintiaDiogène, JorgeCaixach, JosepCampàs, MònicaMagnetic particles (MPs)LC-MS/MShttp://metadata.un.org/sdg/3http://metadata.un.org/sdg/9Ensure healthy lives and promote well-being for all at all agesBuild resilient infrastructure, promote inclusive and sustainable industrialization and foster innovationIn this work, magnetic particles (MPs) are used as supports for the immobilization of biorecognition molecules for the detection of microcystins (MCs). In one approach, a recombinant protein phosphatase 1 (PP1) has been conjugated to MPs via coordination chemistry, and MC-LR detection has been based on the inhibition of the enzyme activity. In the other approach, a monoclonal antibody (mAb) against MC-LR has been conjugated to protein G-coated MPs, and a direct competitive enzyme-linked immunoparticle assay (ELIPA) has been then performed. Conjugation of biomolecules to MPs has been first checked, and after optimization, MC detection has been performed. The colorimetric PPIA with PP1-MP and the best ELIPA strategy have provided limits of detection (LOD) of 7.4 and 3.9 μg/L of MC-LR, respectively. The electrochemical ELIPA has decreased the LOD to 0.4 μg/L, value below the guideline recommended by the World Health Organisation (WHO). The approaches have been applied to the analysis of a cyanobacterial culture and a natural bloom, and MC equivalent contents have been compared to those obtained by conventional assays and liquid chromatography-tandem mass spectrometry (LC-MS/MS). Results have demonstrated the viability of the use of MPs as biomolecule immobilization supports in biotechnological tools for MCs monitoring.This work has been funded by INIA through the RTA2008-00084-00-00 project and by INTERREG SUDOE IVB and FEDER through the SOE1/P1/E129 ALARMTOX project. M.C. acknowledges financial support from the Ministerio de Ciencia e Innovación and the European Social Fund through the Ramón y Cajal program. Diana Garibo acknowledges scholarships from the ALARMTOX project and the Comissionat per a Universitats i Recerca of the Departament d’Innovació, Universitats i Empresa of the Generalitat de Catalunya. The authors acknowledge CRITT-BioIndustries for providing the recombinant PP1 in the frame of the ALARMTOX project, and Dr. Quesada from the Universidad Autónoma de Madrid (Spain) for kindly providing cyanobacteria samples from the Alcántara reservoir.Peer reviewedAmerican Chemical SocietyConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202620262013info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/413894https://api.elsevier.com/content/abstract/scopus_id/84871752751reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésEnvironmental science & technologyhttps://doi.org/10.1021/es304234nSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/4138942026-05-22T06:33:51Z
dc.title.none.fl_str_mv Magnetic particle-based enzyme assays and immunoassays for microcystins: from colorimetric to electrochemical detection
title Magnetic particle-based enzyme assays and immunoassays for microcystins: from colorimetric to electrochemical detection
spellingShingle Magnetic particle-based enzyme assays and immunoassays for microcystins: from colorimetric to electrochemical detection
Reverté, Laia
Magnetic particles (MPs)
LC-MS/MS
http://metadata.un.org/sdg/3
http://metadata.un.org/sdg/9
Ensure healthy lives and promote well-being for all at all ages
Build resilient infrastructure, promote inclusive and sustainable industrialization and foster innovation
title_short Magnetic particle-based enzyme assays and immunoassays for microcystins: from colorimetric to electrochemical detection
title_full Magnetic particle-based enzyme assays and immunoassays for microcystins: from colorimetric to electrochemical detection
title_fullStr Magnetic particle-based enzyme assays and immunoassays for microcystins: from colorimetric to electrochemical detection
title_full_unstemmed Magnetic particle-based enzyme assays and immunoassays for microcystins: from colorimetric to electrochemical detection
title_sort Magnetic particle-based enzyme assays and immunoassays for microcystins: from colorimetric to electrochemical detection
dc.creator.none.fl_str_mv Reverté, Laia
Garibo, Diana
Flores, Cintia
Diogène, Jorge
Caixach, Josep
Campàs, Mònica
author Reverté, Laia
author_facet Reverté, Laia
Garibo, Diana
Flores, Cintia
Diogène, Jorge
Caixach, Josep
Campàs, Mònica
author_role author
author2 Garibo, Diana
Flores, Cintia
Diogène, Jorge
Caixach, Josep
Campàs, Mònica
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Magnetic particles (MPs)
LC-MS/MS
http://metadata.un.org/sdg/3
http://metadata.un.org/sdg/9
Ensure healthy lives and promote well-being for all at all ages
Build resilient infrastructure, promote inclusive and sustainable industrialization and foster innovation
topic Magnetic particles (MPs)
LC-MS/MS
http://metadata.un.org/sdg/3
http://metadata.un.org/sdg/9
Ensure healthy lives and promote well-being for all at all ages
Build resilient infrastructure, promote inclusive and sustainable industrialization and foster innovation
description In this work, magnetic particles (MPs) are used as supports for the immobilization of biorecognition molecules for the detection of microcystins (MCs). In one approach, a recombinant protein phosphatase 1 (PP1) has been conjugated to MPs via coordination chemistry, and MC-LR detection has been based on the inhibition of the enzyme activity. In the other approach, a monoclonal antibody (mAb) against MC-LR has been conjugated to protein G-coated MPs, and a direct competitive enzyme-linked immunoparticle assay (ELIPA) has been then performed. Conjugation of biomolecules to MPs has been first checked, and after optimization, MC detection has been performed. The colorimetric PPIA with PP1-MP and the best ELIPA strategy have provided limits of detection (LOD) of 7.4 and 3.9 μg/L of MC-LR, respectively. The electrochemical ELIPA has decreased the LOD to 0.4 μg/L, value below the guideline recommended by the World Health Organisation (WHO). The approaches have been applied to the analysis of a cyanobacterial culture and a natural bloom, and MC equivalent contents have been compared to those obtained by conventional assays and liquid chromatography-tandem mass spectrometry (LC-MS/MS). Results have demonstrated the viability of the use of MPs as biomolecule immobilization supports in biotechnological tools for MCs monitoring.
publishDate 2013
dc.date.none.fl_str_mv 2013
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/413894
https://api.elsevier.com/content/abstract/scopus_id/84871752751
url http://hdl.handle.net/10261/413894
https://api.elsevier.com/content/abstract/scopus_id/84871752751
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Environmental science & technology
https://doi.org/10.1021/es304234n
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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