Liquid chromatography-mass spectrometry
Since the development of atmospheric pressure ionisation (API), especially the electrospray ionisation (ESI) interface, liquid chromatography-mass spectrometry (LC-MS) has become a routine analytical tool. The strength of LC-MS lies in its ability to selectively determine trace analytes in very comp...
| Autores: | , , , , , , , , |
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| Tipo de recurso: | otro |
| Estado: | Versión aceptada para publicación |
| Fecha de publicación: | 2017 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/414991 |
| Acceso en línea: | http://hdl.handle.net/10261/414991 https://api.elsevier.com/content/abstract/scopus_id/85041524466 |
| Access Level: | acceso abierto |
| Palabra clave: | LC-MS Liquid Chromatography–Mass Spectrometry http://metadata.un.org/sdg/9 http://metadata.un.org/sdg/3 Ensure healthy lives and promote well-being for all at all ages Build resilient infrastructure, promote inclusive and sustainable industrialization and foster innovation |
| Sumario: | Since the development of atmospheric pressure ionisation (API), especially the electrospray ionisation (ESI) interface, liquid chromatography-mass spectrometry (LC-MS) has become a routine analytical tool. The strength of LC-MS lies in its ability to selectively determine trace analytes in very complex sample matrices. The fast scanning speeds of modern MS instruments and the wide selection of MS technologies available, including instruments with the capability to perform tandem MS (MS/MS) and even multigenerational MS (MSn), along with instruments that can provide high mass accuracy, make this technology ideal for identifying multiple target compounds in a single analytical run. These multiple attributes and abilities have made LC-MS a very valuable tool in the characterisation of naturally occurring bioactive molecules and their analogues in a wide range of sample types. However, with use comes knowledge and nowadays LC-MS users realise that the technique is not the panacea they once believed it to be. Matrix-dependent ion suppression can be a significant problem with real samples impinging negatively on critical experimental parameters including LOD, LOQ, linearity, etc. Thankfully researchers have found ways to identify, quantify and circumnavigate this problem; these approaches now form part of the routine protocol employed for the validation of LC-MS-based methods. The objective of this review is to provide an up-to-date overview of the current techniques in LC-MS and its application for cyanobacterial toxin characterisation. |
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