Transcription-mediated supercoiling regulates genome folding and loop formation

The chromatin fiber folds into loops, but the mechanisms controlling loop extrusion are still poorly understood. Using super-resolution microscopy, we visualize that loops in intact nuclei are formed by a scaffold of cohesin complexes from which the DNA protrudes. RNA polymerase II decorates the top...

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Autores: Neguembor, Maria Victoria, Martin, Laura, Castells García, Àlvaro, 1991-, Gómez García, Pablo, Vicario, Chiara, Carnevali, Davide, Abed, Jumana AlHaj, Granados, Alba, Sebastián Pérez, Rubén, Sottile, Francesco, 1988-, Solon, Jérôme, Wu, Chao-Ting, Lakadamyali, Melike, Cosma, Maria Pia, 1970-
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2021
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/48422
Acceso en línea:http://hdl.handle.net/10230/48422
http://dx.doi.org/10.1016/j.molcel.2021.06.009
Access Level:acceso abierto
Palabra clave:STORM microscopy
Cohesin
Genome folding
Super-resolution microscopy
Supercoiling
Transcription
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spelling Transcription-mediated supercoiling regulates genome folding and loop formationNeguembor, Maria VictoriaMartin, LauraCastells García, Àlvaro, 1991-Gómez García, PabloVicario, ChiaraCarnevali, DavideAbed, Jumana AlHajGranados, AlbaSebastián Pérez, RubénSottile, Francesco, 1988-Solon, JérômeWu, Chao-TingLakadamyali, MelikeCosma, Maria Pia, 1970-STORM microscopyCohesinGenome foldingSuper-resolution microscopySupercoilingTranscriptionThe chromatin fiber folds into loops, but the mechanisms controlling loop extrusion are still poorly understood. Using super-resolution microscopy, we visualize that loops in intact nuclei are formed by a scaffold of cohesin complexes from which the DNA protrudes. RNA polymerase II decorates the top of the loops and is physically segregated from cohesin. Augmented looping upon increased loading of cohesin on chromosomes causes disruption of Lamin at the nuclear rim and chromatin blending, a homogeneous distribution of chromatin within the nucleus. Altering supercoiling via either transcription or topoisomerase inhibition counteracts chromatin blending, increases chromatin condensation, disrupts loop formation, and leads to altered cohesin distribution and mobility on chromatin. Overall, negative supercoiling generated by transcription is an important regulator of loop formation in vivo.Elsevier20212021info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/48422http://dx.doi.org/10.1016/j.molcel.2021.06.009reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésMol Cell. 2021;81(15):3065-81.e12© Elsevier http://dx.doi.org/10.1016/j.molcel.2021.06.009info:eu-repo/semantics/openAccessoai:recercat.cat:10230/484222026-05-29T05:05:01Z
dc.title.none.fl_str_mv Transcription-mediated supercoiling regulates genome folding and loop formation
title Transcription-mediated supercoiling regulates genome folding and loop formation
spellingShingle Transcription-mediated supercoiling regulates genome folding and loop formation
Neguembor, Maria Victoria
STORM microscopy
Cohesin
Genome folding
Super-resolution microscopy
Supercoiling
Transcription
title_short Transcription-mediated supercoiling regulates genome folding and loop formation
title_full Transcription-mediated supercoiling regulates genome folding and loop formation
title_fullStr Transcription-mediated supercoiling regulates genome folding and loop formation
title_full_unstemmed Transcription-mediated supercoiling regulates genome folding and loop formation
title_sort Transcription-mediated supercoiling regulates genome folding and loop formation
dc.creator.none.fl_str_mv Neguembor, Maria Victoria
Martin, Laura
Castells García, Àlvaro, 1991-
Gómez García, Pablo
Vicario, Chiara
Carnevali, Davide
Abed, Jumana AlHaj
Granados, Alba
Sebastián Pérez, Rubén
Sottile, Francesco, 1988-
Solon, Jérôme
Wu, Chao-Ting
Lakadamyali, Melike
Cosma, Maria Pia, 1970-
author Neguembor, Maria Victoria
author_facet Neguembor, Maria Victoria
Martin, Laura
Castells García, Àlvaro, 1991-
Gómez García, Pablo
Vicario, Chiara
Carnevali, Davide
Abed, Jumana AlHaj
Granados, Alba
Sebastián Pérez, Rubén
Sottile, Francesco, 1988-
Solon, Jérôme
Wu, Chao-Ting
Lakadamyali, Melike
Cosma, Maria Pia, 1970-
author_role author
author2 Martin, Laura
Castells García, Àlvaro, 1991-
Gómez García, Pablo
Vicario, Chiara
Carnevali, Davide
Abed, Jumana AlHaj
Granados, Alba
Sebastián Pérez, Rubén
Sottile, Francesco, 1988-
Solon, Jérôme
Wu, Chao-Ting
Lakadamyali, Melike
Cosma, Maria Pia, 1970-
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv STORM microscopy
Cohesin
Genome folding
Super-resolution microscopy
Supercoiling
Transcription
topic STORM microscopy
Cohesin
Genome folding
Super-resolution microscopy
Supercoiling
Transcription
description The chromatin fiber folds into loops, but the mechanisms controlling loop extrusion are still poorly understood. Using super-resolution microscopy, we visualize that loops in intact nuclei are formed by a scaffold of cohesin complexes from which the DNA protrudes. RNA polymerase II decorates the top of the loops and is physically segregated from cohesin. Augmented looping upon increased loading of cohesin on chromosomes causes disruption of Lamin at the nuclear rim and chromatin blending, a homogeneous distribution of chromatin within the nucleus. Altering supercoiling via either transcription or topoisomerase inhibition counteracts chromatin blending, increases chromatin condensation, disrupts loop formation, and leads to altered cohesin distribution and mobility on chromatin. Overall, negative supercoiling generated by transcription is an important regulator of loop formation in vivo.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/48422
http://dx.doi.org/10.1016/j.molcel.2021.06.009
url http://hdl.handle.net/10230/48422
http://dx.doi.org/10.1016/j.molcel.2021.06.009
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Mol Cell. 2021;81(15):3065-81.e12
dc.rights.none.fl_str_mv © Elsevier http://dx.doi.org/10.1016/j.molcel.2021.06.009
info:eu-repo/semantics/openAccess
rights_invalid_str_mv © Elsevier http://dx.doi.org/10.1016/j.molcel.2021.06.009
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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