Identification of Genes Responsive to Solar Simulated UV Radiation in Human Monocyte-Derived Dendritic Cells

Ultraviolet (UV) irradiation has profound effects on the skin and the systemic immune system. Several effects of UV radiation on Dendritic cells (DCs) functions have been described. However, gene expression changes induced by UV radiation in DCs have not been addressed before. In this report, we irr...

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Detalles Bibliográficos
Autores: Fuente, Hortensia de la, Lamana Domínguez, Amalia, Mittelbrunn, María, Pérez-Gala, Silvia, González, Salvador, García-Diez, Amaro, Vega, Miguel, Sánchez-Madrid, Francisco
Tipo de recurso: artículo
Fecha de publicación:2009
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/94243
Acceso en línea:https://hdl.handle.net/20.500.14352/94243
Access Level:acceso abierto
Palabra clave:576.3
577.21
577.27
Genes
Dendritic Cells
UV Radiation
Biología celular (Biología)
Genética
Inmunología
2407 Biología Celular
2409 Genética
2412 Inmunología
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oai_identifier_str oai:docta.ucm.es:20.500.14352/94243
network_acronym_str ES
network_name_str España
repository_id_str
spelling Identification of Genes Responsive to Solar Simulated UV Radiation in Human Monocyte-Derived Dendritic CellsFuente, Hortensia de laLamana Domínguez, AmaliaMittelbrunn, MaríaPérez-Gala, SilviaGonzález, SalvadorGarcía-Diez, AmaroVega, MiguelSánchez-Madrid, Francisco576.3577.21577.27GenesDendritic CellsUV RadiationBiología celular (Biología)GenéticaInmunología2407 Biología Celular2409 Genética2412 InmunologíaUltraviolet (UV) irradiation has profound effects on the skin and the systemic immune system. Several effects of UV radiation on Dendritic cells (DCs) functions have been described. However, gene expression changes induced by UV radiation in DCs have not been addressed before. In this report, we irradiated human monocyte-derived DCs with solar-simulated UVA/UVB and analyzed regulated genes on human whole genome arrays. Results were validated by RT-PCR and further analyzed by Gene Set Enrichment Analysis (GSEA). Solar-simulated UV radiation up-regulated expression of genes involved in cellular stress and inflammation, and down-regulated genes involved in chemotaxis, vesicular transport and RNA processing. Twenty four genes were selected for comparison by RT-PCR with similarly treated human primary keratinocytes and human melanocytes. Several genes involved in the regulation of the immune response were differentially regulated in UVA/UVB irradiated human monocyte-derived DCs, such as protein tyrosine phosphatase, receptor type E (PTPRE), thrombospondin-1 (THBS1), inducible costimulator ligand (ICOSL), galectins, Src-like adapter protein (SLA), IL-10 and CCR7. These results indicate that UV-exposure triggers the regulation of a complex gene repertoire involved in human-DC–mediated immune responses.Public Library of ScienceSebastian D. FugmannUniversidad Complutense de Madrid20092009-01-0120092009-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/94243reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/942432026-06-02T12:44:21Z
dc.title.none.fl_str_mv Identification of Genes Responsive to Solar Simulated UV Radiation in Human Monocyte-Derived Dendritic Cells
title Identification of Genes Responsive to Solar Simulated UV Radiation in Human Monocyte-Derived Dendritic Cells
spellingShingle Identification of Genes Responsive to Solar Simulated UV Radiation in Human Monocyte-Derived Dendritic Cells
Fuente, Hortensia de la
576.3
577.21
577.27
Genes
Dendritic Cells
UV Radiation
Biología celular (Biología)
Genética
Inmunología
2407 Biología Celular
2409 Genética
2412 Inmunología
title_short Identification of Genes Responsive to Solar Simulated UV Radiation in Human Monocyte-Derived Dendritic Cells
title_full Identification of Genes Responsive to Solar Simulated UV Radiation in Human Monocyte-Derived Dendritic Cells
title_fullStr Identification of Genes Responsive to Solar Simulated UV Radiation in Human Monocyte-Derived Dendritic Cells
title_full_unstemmed Identification of Genes Responsive to Solar Simulated UV Radiation in Human Monocyte-Derived Dendritic Cells
title_sort Identification of Genes Responsive to Solar Simulated UV Radiation in Human Monocyte-Derived Dendritic Cells
dc.creator.none.fl_str_mv Fuente, Hortensia de la
Lamana Domínguez, Amalia
Mittelbrunn, María
Pérez-Gala, Silvia
González, Salvador
García-Diez, Amaro
Vega, Miguel
Sánchez-Madrid, Francisco
author Fuente, Hortensia de la
author_facet Fuente, Hortensia de la
Lamana Domínguez, Amalia
Mittelbrunn, María
Pérez-Gala, Silvia
González, Salvador
García-Diez, Amaro
Vega, Miguel
Sánchez-Madrid, Francisco
author_role author
author2 Lamana Domínguez, Amalia
Mittelbrunn, María
Pérez-Gala, Silvia
González, Salvador
García-Diez, Amaro
Vega, Miguel
Sánchez-Madrid, Francisco
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Sebastian D. Fugmann
Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 576.3
577.21
577.27
Genes
Dendritic Cells
UV Radiation
Biología celular (Biología)
Genética
Inmunología
2407 Biología Celular
2409 Genética
2412 Inmunología
topic 576.3
577.21
577.27
Genes
Dendritic Cells
UV Radiation
Biología celular (Biología)
Genética
Inmunología
2407 Biología Celular
2409 Genética
2412 Inmunología
description Ultraviolet (UV) irradiation has profound effects on the skin and the systemic immune system. Several effects of UV radiation on Dendritic cells (DCs) functions have been described. However, gene expression changes induced by UV radiation in DCs have not been addressed before. In this report, we irradiated human monocyte-derived DCs with solar-simulated UVA/UVB and analyzed regulated genes on human whole genome arrays. Results were validated by RT-PCR and further analyzed by Gene Set Enrichment Analysis (GSEA). Solar-simulated UV radiation up-regulated expression of genes involved in cellular stress and inflammation, and down-regulated genes involved in chemotaxis, vesicular transport and RNA processing. Twenty four genes were selected for comparison by RT-PCR with similarly treated human primary keratinocytes and human melanocytes. Several genes involved in the regulation of the immune response were differentially regulated in UVA/UVB irradiated human monocyte-derived DCs, such as protein tyrosine phosphatase, receptor type E (PTPRE), thrombospondin-1 (THBS1), inducible costimulator ligand (ICOSL), galectins, Src-like adapter protein (SLA), IL-10 and CCR7. These results indicate that UV-exposure triggers the regulation of a complex gene repertoire involved in human-DC–mediated immune responses.
publishDate 2009
dc.date.none.fl_str_mv 2009
2009-01-01
2009
2009-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/94243
url https://hdl.handle.net/20.500.14352/94243
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869421053926703104
score 15,301603

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