CD69 Modulates Sphingosine-1-Phosphate-Induced Migration of Skin Dendritic Cells

In this study, we have investigated the role of CD69, an early inducible leukocyte activation receptor, in murine dendritic cell (DC) differentiation, maturation, and migration. Skin DCs and DC subsets present in mouse lymphoid organs express CD69 in response to maturation stimuli. Using a contact s...

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Detalles Bibliográficos
Autores: Lamana Domínguez, Amalia, Martin, Pilar, Fuente, Hortensia de la, Martinez-Muñoz, Laura, Cruz Adalia, Aranzazu, Ramirez-Huesca, Marta, Escribano, Cristina, Gollmer, Kathrin, Mellado, Mario, Stein, Jens, Rodriguez-Fernandez, Jose Luis, Sanchez-Madrid, Francisco, Martinez del Hoyo, Gloria
Tipo de recurso: artículo
Fecha de publicación:2011
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/94309
Acceso en línea:https://hdl.handle.net/20.500.14352/94309
Access Level:acceso abierto
Palabra clave:576.3
577.27
616.5
CD69
Skin Dendritic Cells
Biología celular (Biología)
Inmunología
Dermatología
2407 Biología Celular
2412 Inmunología
3201.06 Dermatología
Descripción
Sumario:In this study, we have investigated the role of CD69, an early inducible leukocyte activation receptor, in murine dendritic cell (DC) differentiation, maturation, and migration. Skin DCs and DC subsets present in mouse lymphoid organs express CD69 in response to maturation stimuli. Using a contact sensitization model, we show that skin DCs migrated more efficiently to draining lymph nodes (LNs) in the absence of CD69. This was confirmed by subcutaneous transfer of CD69–/– DCs, which presented an increased migration to peripheral LNs. Two-photon microscopy analysis showed that once DCs reached the LNs, CD69 deficiency did not alter DC interstitial motility in the LNs. Chemotaxis to sphingosine-1-phosphate (S1P) was enhanced in CD69–/– DCs compared with wild-type DCs. Accordingly, we detected a higher expression of S1P receptor type-1 (S1P1) by CD69–/– DCs, whereas S1P3 expression levels were similar in wild-type and CD69–/– DCs. Moreover, in vivo treatment with S1P analogs SEW2871 and FTY720 during skin sensitization reduced skin DC migration to peripheral LNs. These results suggest that CD69 regulates S1P-induced skin DC migration by modulating S1P1 function. Together, our findings increase our knowledge on DC trafficking patterns in the skin, enabling the development of new directed therapies using DCs for antigen (Ag) delivery.

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