Functional Role of P-Selectin Glycoprotein Ligand 1/P-Selectin Interaction in the Generation of Tolerogenic Dendritic Cells

Dendritic cells (DCs) have a key role in both the generation of the immune response and the induction of tolerance to self-Ags. In this work, the possible role of P-selectin glycoprotein ligand 1 (PSGL-1) on the tolerogenic activity of human DCs was explored. We found that the engagement of PSGL-1 b...

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Detalles Bibliográficos
Autores: Urzainqui, Ana, Martínez del Hoyo, Gloria, Lamana Domínguez, Amalia, Fuente, Hortensia de la, Barreiro, Olga, Olazabal, Isabel, Pilar Martin, Wild, Martin, Vestweber, Dietmar, González-Amaro, Roberto, Sánchez-Madrid, Francisco
Tipo de recurso: artículo
Fecha de publicación:2007
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/94052
Acceso en línea:https://hdl.handle.net/20.500.14352/94052
Access Level:acceso abierto
Palabra clave:576.3
577.27
P-Selectin Glycoprotein Ligand 1
Dendritic Cells
Biología celular (Biología)
Inmunología
2407 Biología Celular
2412 Inmunología
id ES_d61bf1ea9f5ec09b3b3c9afe2a4b73d7
oai_identifier_str oai:docta.ucm.es:20.500.14352/94052
network_acronym_str ES
network_name_str España
repository_id_str
spelling Functional Role of P-Selectin Glycoprotein Ligand 1/P-Selectin Interaction in the Generation of Tolerogenic Dendritic CellsUrzainqui, AnaMartínez del Hoyo, GloriaLamana Domínguez, AmaliaFuente, Hortensia de laBarreiro, OlgaOlazabal, IsabelPilar MartinWild, MartinVestweber, DietmarGonzález-Amaro, RobertoSánchez-Madrid, Francisco576.3577.27P-Selectin Glycoprotein Ligand 1Dendritic CellsBiología celular (Biología)Inmunología2407 Biología Celular2412 InmunologíaDendritic cells (DCs) have a key role in both the generation of the immune response and the induction of tolerance to self-Ags. In this work, the possible role of P-selectin glycoprotein ligand 1 (PSGL-1) on the tolerogenic activity of human DCs was explored. We found that the engagement of PSGL-1 by P-selectin on DCs induced the expression of c-Fos, IDO, IL-10, and TGF-β genes. Remarkably, stimulation of DCs through PSGL-1 with P-selectin enhanced their capability to generate CD4+CD25+Foxp3+ regulatory T cells, which expressed high levels of TGF-β1 mRNA, synthesized IL-10, and suppressed the proliferation of autologous CD4+CD25− T cells. Accordingly, we found that DCs from PSGL-1−/− mice expressed higher levels of MHC class II molecules, and exhibited an enhanced immunogenicity compared with wild-type mice. In addition, the percentage of CD4+CD25+Foxp3+ regulatory T cells in the thymus of PSGL-1-deficient animals was significantly reduced. Our data reveal an unexpected role of PSGL-1 on the tolerogenic function of DCs, and the regulation of the immune response.American Association of ImmunologistsUniversidad Complutense de Madrid20072007-01-0120072007-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/94052reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/940522026-06-02T12:44:21Z
dc.title.none.fl_str_mv Functional Role of P-Selectin Glycoprotein Ligand 1/P-Selectin Interaction in the Generation of Tolerogenic Dendritic Cells
title Functional Role of P-Selectin Glycoprotein Ligand 1/P-Selectin Interaction in the Generation of Tolerogenic Dendritic Cells
spellingShingle Functional Role of P-Selectin Glycoprotein Ligand 1/P-Selectin Interaction in the Generation of Tolerogenic Dendritic Cells
Urzainqui, Ana
576.3
577.27
P-Selectin Glycoprotein Ligand 1
Dendritic Cells
Biología celular (Biología)
Inmunología
2407 Biología Celular
2412 Inmunología
title_short Functional Role of P-Selectin Glycoprotein Ligand 1/P-Selectin Interaction in the Generation of Tolerogenic Dendritic Cells
title_full Functional Role of P-Selectin Glycoprotein Ligand 1/P-Selectin Interaction in the Generation of Tolerogenic Dendritic Cells
title_fullStr Functional Role of P-Selectin Glycoprotein Ligand 1/P-Selectin Interaction in the Generation of Tolerogenic Dendritic Cells
title_full_unstemmed Functional Role of P-Selectin Glycoprotein Ligand 1/P-Selectin Interaction in the Generation of Tolerogenic Dendritic Cells
title_sort Functional Role of P-Selectin Glycoprotein Ligand 1/P-Selectin Interaction in the Generation of Tolerogenic Dendritic Cells
dc.creator.none.fl_str_mv Urzainqui, Ana
Martínez del Hoyo, Gloria
Lamana Domínguez, Amalia
Fuente, Hortensia de la
Barreiro, Olga
Olazabal, Isabel
Pilar Martin
Wild, Martin
Vestweber, Dietmar
González-Amaro, Roberto
Sánchez-Madrid, Francisco
author Urzainqui, Ana
author_facet Urzainqui, Ana
Martínez del Hoyo, Gloria
Lamana Domínguez, Amalia
Fuente, Hortensia de la
Barreiro, Olga
Olazabal, Isabel
Pilar Martin
Wild, Martin
Vestweber, Dietmar
González-Amaro, Roberto
Sánchez-Madrid, Francisco
author_role author
author2 Martínez del Hoyo, Gloria
Lamana Domínguez, Amalia
Fuente, Hortensia de la
Barreiro, Olga
Olazabal, Isabel
Pilar Martin
Wild, Martin
Vestweber, Dietmar
González-Amaro, Roberto
Sánchez-Madrid, Francisco
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 576.3
577.27
P-Selectin Glycoprotein Ligand 1
Dendritic Cells
Biología celular (Biología)
Inmunología
2407 Biología Celular
2412 Inmunología
topic 576.3
577.27
P-Selectin Glycoprotein Ligand 1
Dendritic Cells
Biología celular (Biología)
Inmunología
2407 Biología Celular
2412 Inmunología
description Dendritic cells (DCs) have a key role in both the generation of the immune response and the induction of tolerance to self-Ags. In this work, the possible role of P-selectin glycoprotein ligand 1 (PSGL-1) on the tolerogenic activity of human DCs was explored. We found that the engagement of PSGL-1 by P-selectin on DCs induced the expression of c-Fos, IDO, IL-10, and TGF-β genes. Remarkably, stimulation of DCs through PSGL-1 with P-selectin enhanced their capability to generate CD4+CD25+Foxp3+ regulatory T cells, which expressed high levels of TGF-β1 mRNA, synthesized IL-10, and suppressed the proliferation of autologous CD4+CD25− T cells. Accordingly, we found that DCs from PSGL-1−/− mice expressed higher levels of MHC class II molecules, and exhibited an enhanced immunogenicity compared with wild-type mice. In addition, the percentage of CD4+CD25+Foxp3+ regulatory T cells in the thymus of PSGL-1-deficient animals was significantly reduced. Our data reveal an unexpected role of PSGL-1 on the tolerogenic function of DCs, and the regulation of the immune response.
publishDate 2007
dc.date.none.fl_str_mv 2007
2007-01-01
2007
2007-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/94052
url https://hdl.handle.net/20.500.14352/94052
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Association of Immunologists
publisher.none.fl_str_mv American Association of Immunologists
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869420807931822080
score 15.812429

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