High production of transfer RNAs identifies the presence of developing oocytes in ovaries and intersex testes of teleost fish

5S rRNA is highly transcribed in fish oocytes and this transcription levels can be used to identify the presence of oocytes in the intersex testes of fish exposed to xenoestrogens. Similar to 5S rRNA, tRNAs are transcribed by RNA polymerase III (Pol-III) in eukaryotes, so this study focuses in the a...

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Autores: Bir, Joyanta, Rojo Bartolomé, Iratxe, Lecube Iturrioz, Xabier, Diaz de Cerio Arruabarrena, Oihane, Ortiz Zarragoitia, Maren, Cancio Uriarte, Ibon
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/59917
Acceso en línea:http://hdl.handle.net/10810/59917
Access Level:acceso abierto
Palabra clave:oogenesis
intersex condition
tRNA
tRNA/5.8S rRNA index
miRNAs
RNA
polymerase III
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spelling High production of transfer RNAs identifies the presence of developing oocytes in ovaries and intersex testes of teleost fishBir, JoyantaRojo Bartolomé, IratxeLecube Iturrioz, XabierDiaz de Cerio Arruabarrena, OihaneOrtiz Zarragoitia, MarenCancio Uriarte, Ibonoogenesisintersex conditiontRNAtRNA/5.8S rRNA indexmiRNAsRNApolymerase III5S rRNA is highly transcribed in fish oocytes and this transcription levels can be used to identify the presence of oocytes in the intersex testes of fish exposed to xenoestrogens. Similar to 5S rRNA, tRNAs are transcribed by RNA polymerase III (Pol-III) in eukaryotes, so this study focuses in the analysis of the levels of expression of tRNAs in the gonads (ovaries and testes) of eight teleost species as a possible new oocyte molecular marker. Total RNA extracted from gonads of six commercial teleost species in the Biscay Bay, from the pollution sentinel species thicklip grey mullet (Chelon labrosus) known present intersex testes in response to xenoestrogens in Gernika estuary and from the laboratory model species Danio rerio were analysed through capillary electrophoresis. Bioanalyzer electropherograms were used to quantify the concentrations of tRNAs, 5S and 5.8S rRNA. All studied ovaries expressed significantly higher levels of tRNAs and 5S rRNA than testes. A tRNA to 5.8S rRNA index was calculated which differentiates ovaries from testes, and identifies some intersex testes in between testes and ovaries in mullets. The tRNA/5.8S ratio was highest in ovaries in previtellogenic stage, decreasing towards maturity. Thus, strong oocyte expression of tRNAs is an additional proof of high activity levels of Pol-III during early stages of oocyte development in teleost ovaries. Incidentally, we observed that miRNA concentrations were always higher in testes than ovaries. The indexing approach developed in the present study could have multiple applications in teleost reproduction research and in the development of early molecular markers of intersex condition.This work has been supported by project Born2bEgg (Spanish MCIN & EU-FEDER/ERDF (PGC2018-101442-B-100) and by the grants to consolidated research projects of the Basque Government (IT1302-19 and IT1743-22). We are thankful to the Biscay Bay Environmental Biospecimen Bank (BBEBB; PiE-UPV/EHU) for providing mullet tissues.Elsevier202320232023info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/59917reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoInglésinfo:eu-repo/grantAgreement/MICIU/PGC2018-101442-B-100/https://www.sciencedirect.com/science/article/pii/S0141113623000351?via%3Dihubinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/3.0/es/© 2023 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).Atribución 3.0 Españaoai:addi.ehu.eus:10810/599172026-06-18T09:23:17Z
dc.title.none.fl_str_mv High production of transfer RNAs identifies the presence of developing oocytes in ovaries and intersex testes of teleost fish
title High production of transfer RNAs identifies the presence of developing oocytes in ovaries and intersex testes of teleost fish
spellingShingle High production of transfer RNAs identifies the presence of developing oocytes in ovaries and intersex testes of teleost fish
Bir, Joyanta
oogenesis
intersex condition
tRNA
tRNA/5.8S rRNA index
miRNAs
RNA
polymerase III
title_short High production of transfer RNAs identifies the presence of developing oocytes in ovaries and intersex testes of teleost fish
title_full High production of transfer RNAs identifies the presence of developing oocytes in ovaries and intersex testes of teleost fish
title_fullStr High production of transfer RNAs identifies the presence of developing oocytes in ovaries and intersex testes of teleost fish
title_full_unstemmed High production of transfer RNAs identifies the presence of developing oocytes in ovaries and intersex testes of teleost fish
title_sort High production of transfer RNAs identifies the presence of developing oocytes in ovaries and intersex testes of teleost fish
dc.creator.none.fl_str_mv Bir, Joyanta
Rojo Bartolomé, Iratxe
Lecube Iturrioz, Xabier
Diaz de Cerio Arruabarrena, Oihane
Ortiz Zarragoitia, Maren
Cancio Uriarte, Ibon
author Bir, Joyanta
author_facet Bir, Joyanta
Rojo Bartolomé, Iratxe
Lecube Iturrioz, Xabier
Diaz de Cerio Arruabarrena, Oihane
Ortiz Zarragoitia, Maren
Cancio Uriarte, Ibon
author_role author
author2 Rojo Bartolomé, Iratxe
Lecube Iturrioz, Xabier
Diaz de Cerio Arruabarrena, Oihane
Ortiz Zarragoitia, Maren
Cancio Uriarte, Ibon
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv oogenesis
intersex condition
tRNA
tRNA/5.8S rRNA index
miRNAs
RNA
polymerase III
topic oogenesis
intersex condition
tRNA
tRNA/5.8S rRNA index
miRNAs
RNA
polymerase III
description 5S rRNA is highly transcribed in fish oocytes and this transcription levels can be used to identify the presence of oocytes in the intersex testes of fish exposed to xenoestrogens. Similar to 5S rRNA, tRNAs are transcribed by RNA polymerase III (Pol-III) in eukaryotes, so this study focuses in the analysis of the levels of expression of tRNAs in the gonads (ovaries and testes) of eight teleost species as a possible new oocyte molecular marker. Total RNA extracted from gonads of six commercial teleost species in the Biscay Bay, from the pollution sentinel species thicklip grey mullet (Chelon labrosus) known present intersex testes in response to xenoestrogens in Gernika estuary and from the laboratory model species Danio rerio were analysed through capillary electrophoresis. Bioanalyzer electropherograms were used to quantify the concentrations of tRNAs, 5S and 5.8S rRNA. All studied ovaries expressed significantly higher levels of tRNAs and 5S rRNA than testes. A tRNA to 5.8S rRNA index was calculated which differentiates ovaries from testes, and identifies some intersex testes in between testes and ovaries in mullets. The tRNA/5.8S ratio was highest in ovaries in previtellogenic stage, decreasing towards maturity. Thus, strong oocyte expression of tRNAs is an additional proof of high activity levels of Pol-III during early stages of oocyte development in teleost ovaries. Incidentally, we observed that miRNA concentrations were always higher in testes than ovaries. The indexing approach developed in the present study could have multiple applications in teleost reproduction research and in the development of early molecular markers of intersex condition.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/59917
url http://hdl.handle.net/10810/59917
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MICIU/PGC2018-101442-B-100/
https://www.sciencedirect.com/science/article/pii/S0141113623000351?via%3Dihub
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/3.0/es/
Atribución 3.0 España
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/3.0/es/
Atribución 3.0 España
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301603

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