CRISPR-Cas12b enables a highly efficient attack on HIV proviral DNA in T cell cultures

The novel endonuclease Cas12b was engineered for targeted genome editing in mammalian cells and is a promising tool for certain applications because of its small size, high sequence specificity and ability to generate relatively large deletions. We previously reported inhibition of the human immunod...

Descripción completa

Detalles Bibliográficos
Autores: Fan, Minghui, Bao, Yuanling, Berkhout, Ben, Herrera, Elena
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/405699
Acceso en línea:http://hdl.handle.net/10261/405699
https://api.elsevier.com/content/abstract/scopus_id/85163454716
Access Level:acceso abierto
Palabra clave:Antiviral
CRISPR-Cas12b
HIV cure
Lentiviral vector (LV)
id ES_d2157909b4ececd0b202a6f247748bfa
oai_identifier_str oai:digital.csic.es:10261/405699
network_acronym_str ES
network_name_str España
repository_id_str
spelling CRISPR-Cas12b enables a highly efficient attack on HIV proviral DNA in T cell culturesFan, MinghuiBao, YuanlingBerkhout, BenHerrera, ElenaAntiviralCRISPR-Cas12bHIV cureLentiviral vector (LV)The novel endonuclease Cas12b was engineered for targeted genome editing in mammalian cells and is a promising tool for certain applications because of its small size, high sequence specificity and ability to generate relatively large deletions. We previously reported inhibition of the human immunodeficiency virus (HIV) in cell culture infections upon attack of the integrated viral DNA genome by spCas9 and Cas12a.MF and YB are the recipients of the Chinese Government Scholarships (CSC). This work was supported by NIH RO1 grant 1R01AI145045IH.Peer reviewedElsevierChina Scholarship CouncilNational Institutes of Health (US)Fan, Minghui [0000-0002-2236-5479]202520252023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/405699https://api.elsevier.com/content/abstract/scopus_id/85163454716reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésThe underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1016/j.biopha.2023.115046https://doi.org/10.1016/j.biopha.2023.115046Noinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/4056992026-05-22T06:33:51Z
dc.title.none.fl_str_mv CRISPR-Cas12b enables a highly efficient attack on HIV proviral DNA in T cell cultures
title CRISPR-Cas12b enables a highly efficient attack on HIV proviral DNA in T cell cultures
spellingShingle CRISPR-Cas12b enables a highly efficient attack on HIV proviral DNA in T cell cultures
Fan, Minghui
Antiviral
CRISPR-Cas12b
HIV cure
Lentiviral vector (LV)
title_short CRISPR-Cas12b enables a highly efficient attack on HIV proviral DNA in T cell cultures
title_full CRISPR-Cas12b enables a highly efficient attack on HIV proviral DNA in T cell cultures
title_fullStr CRISPR-Cas12b enables a highly efficient attack on HIV proviral DNA in T cell cultures
title_full_unstemmed CRISPR-Cas12b enables a highly efficient attack on HIV proviral DNA in T cell cultures
title_sort CRISPR-Cas12b enables a highly efficient attack on HIV proviral DNA in T cell cultures
dc.creator.none.fl_str_mv Fan, Minghui
Bao, Yuanling
Berkhout, Ben
Herrera, Elena
author Fan, Minghui
author_facet Fan, Minghui
Bao, Yuanling
Berkhout, Ben
Herrera, Elena
author_role author
author2 Bao, Yuanling
Berkhout, Ben
Herrera, Elena
author2_role author
author
author
dc.contributor.none.fl_str_mv China Scholarship Council
National Institutes of Health (US)
Fan, Minghui [0000-0002-2236-5479]
dc.subject.none.fl_str_mv Antiviral
CRISPR-Cas12b
HIV cure
Lentiviral vector (LV)
topic Antiviral
CRISPR-Cas12b
HIV cure
Lentiviral vector (LV)
description The novel endonuclease Cas12b was engineered for targeted genome editing in mammalian cells and is a promising tool for certain applications because of its small size, high sequence specificity and ability to generate relatively large deletions. We previously reported inhibition of the human immunodeficiency virus (HIV) in cell culture infections upon attack of the integrated viral DNA genome by spCas9 and Cas12a.
publishDate 2023
dc.date.none.fl_str_mv 2023
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/405699
https://api.elsevier.com/content/abstract/scopus_id/85163454716
url http://hdl.handle.net/10261/405699
https://api.elsevier.com/content/abstract/scopus_id/85163454716
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1016/j.biopha.2023.115046
https://doi.org/10.1016/j.biopha.2023.115046
No
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869420319080448000
score 15.81155

Similares en LA Referencia