Light-sheet microscopy imaging of a whole cleared rat brain with Thy1-GFP transgene

Whole-brain imaging with light-sheet fluorescence microscopy and optically cleared tissue is a new, rapidly developing research field. Whereas successful attempts to clear and image mouse brain have been reported, a similar result for rats has proven difficult to achieve. Herein, we report on creati...

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Autores: Gualda Manzano, Emilio José|||0000-0001-5948-5493, Pawloswka, Monika, Marzena, Stefanivk, Legutko, Diana, Matryba, Pawel, Koze, Paulina, Konopka, Witold
Tipo de recurso: artículo
Fecha de publicación:2016
País:España
Institución:Universitat Politècnica de Catalunya (UPC)
Repositorio:UPCommons. Portal del coneixement obert de la UPC
Idioma:inglés
OAI Identifier:oai:upcommons.upc.edu:2117/353497
Acceso en línea:https://hdl.handle.net/2117/353497
https://dx.doi.org/10.1038/srep28209
Access Level:acceso abierto
Palabra clave:Fluorescence microscopy
Light microscopy
Microscòpia de fluorescència
Àrees temàtiques de la UPC::Enginyeria de la telecomunicació::Telecomunicació òptica::Fotònica
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repository_id_str
spelling Light-sheet microscopy imaging of a whole cleared rat brain with Thy1-GFP transgeneGualda Manzano, Emilio José|||0000-0001-5948-5493Pawloswka, MonikaMarzena, StefanivkLegutko, DianaMatryba, PawelKoze, PaulinaKonopka, WitoldFluorescence microscopyLight microscopyMicroscòpia de fluorescènciaÀrees temàtiques de la UPC::Enginyeria de la telecomunicació::Telecomunicació òptica::FotònicaWhole-brain imaging with light-sheet fluorescence microscopy and optically cleared tissue is a new, rapidly developing research field. Whereas successful attempts to clear and image mouse brain have been reported, a similar result for rats has proven difficult to achieve. Herein, we report on creating novel transgenic rat harboring fluorescent reporter GFP under control of neuronal gene promoter. We then present data on clearing the rat brain, showing that FluoClearBABB was found superior over passive CLARITY and CUBIC methodsNature20162016-09-0120212021-10-13journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/2117/353497https://dx.doi.org/10.1038/srep28209reponame:UPCommons. Portal del coneixement obert de la UPCinstname:Universitat Politècnica de Catalunya (UPC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivs 3.0 Spainhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:upcommons.upc.edu:2117/3534972026-05-27T15:37:01Z
dc.title.none.fl_str_mv Light-sheet microscopy imaging of a whole cleared rat brain with Thy1-GFP transgene
title Light-sheet microscopy imaging of a whole cleared rat brain with Thy1-GFP transgene
spellingShingle Light-sheet microscopy imaging of a whole cleared rat brain with Thy1-GFP transgene
Gualda Manzano, Emilio José|||0000-0001-5948-5493
Fluorescence microscopy
Light microscopy
Microscòpia de fluorescència
Àrees temàtiques de la UPC::Enginyeria de la telecomunicació::Telecomunicació òptica::Fotònica
title_short Light-sheet microscopy imaging of a whole cleared rat brain with Thy1-GFP transgene
title_full Light-sheet microscopy imaging of a whole cleared rat brain with Thy1-GFP transgene
title_fullStr Light-sheet microscopy imaging of a whole cleared rat brain with Thy1-GFP transgene
title_full_unstemmed Light-sheet microscopy imaging of a whole cleared rat brain with Thy1-GFP transgene
title_sort Light-sheet microscopy imaging of a whole cleared rat brain with Thy1-GFP transgene
dc.creator.none.fl_str_mv Gualda Manzano, Emilio José|||0000-0001-5948-5493
Pawloswka, Monika
Marzena, Stefanivk
Legutko, Diana
Matryba, Pawel
Koze, Paulina
Konopka, Witold
author Gualda Manzano, Emilio José|||0000-0001-5948-5493
author_facet Gualda Manzano, Emilio José|||0000-0001-5948-5493
Pawloswka, Monika
Marzena, Stefanivk
Legutko, Diana
Matryba, Pawel
Koze, Paulina
Konopka, Witold
author_role author
author2 Pawloswka, Monika
Marzena, Stefanivk
Legutko, Diana
Matryba, Pawel
Koze, Paulina
Konopka, Witold
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Fluorescence microscopy
Light microscopy
Microscòpia de fluorescència
Àrees temàtiques de la UPC::Enginyeria de la telecomunicació::Telecomunicació òptica::Fotònica
topic Fluorescence microscopy
Light microscopy
Microscòpia de fluorescència
Àrees temàtiques de la UPC::Enginyeria de la telecomunicació::Telecomunicació òptica::Fotònica
description Whole-brain imaging with light-sheet fluorescence microscopy and optically cleared tissue is a new, rapidly developing research field. Whereas successful attempts to clear and image mouse brain have been reported, a similar result for rats has proven difficult to achieve. Herein, we report on creating novel transgenic rat harboring fluorescent reporter GFP under control of neuronal gene promoter. We then present data on clearing the rat brain, showing that FluoClearBABB was found superior over passive CLARITY and CUBIC methods
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-09-01
2021
2021-10-13
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/2117/353497
https://dx.doi.org/10.1038/srep28209
url https://hdl.handle.net/2117/353497
https://dx.doi.org/10.1038/srep28209
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature
publisher.none.fl_str_mv Nature
dc.source.none.fl_str_mv reponame:UPCommons. Portal del coneixement obert de la UPC
instname:Universitat Politècnica de Catalunya (UPC)
instname_str Universitat Politècnica de Catalunya (UPC)
reponame_str UPCommons. Portal del coneixement obert de la UPC
collection UPCommons. Portal del coneixement obert de la UPC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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