Neurosporaxanthin Overproduction by Fusarium fujikuroi and Evaluation of Its Antioxidant Properties

17 Páginas.-- 5 Figuras

Detalles Bibliográficos
Autores: Parra-Rivero, Obdulia, Paes-de Barros, Marcelo, Prado, María del Mar, Gil, José Vicente, Hornero-Méndez, Dámaso, Zacarías, Lorenzo, Rodrigo, María Jesús, Limón, M. Carmen, Ávalos, Javier
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/215544
Acceso en línea:http://hdl.handle.net/10261/215544
Access Level:acceso abierto
Palabra clave:Carotenoids
Xanthophylls
Free radicals
Oxidative stress
Fungi
Quenching
Liposomes
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spelling Neurosporaxanthin Overproduction by Fusarium fujikuroi and Evaluation of Its Antioxidant PropertiesParra-Rivero, ObduliaPaes-de Barros, MarceloPrado, María del MarGil, José VicenteHornero-Méndez, DámasoZacarías, LorenzoRodrigo, María JesúsLimón, M. CarmenÁvalos, JavierCarotenoidsXanthophyllsFree radicalsOxidative stressFungiQuenchingLiposomes17 Páginas.-- 5 FigurasNeurosporaxanthin (NX) is a carboxylic carotenoid produced by some filamentous fungi, including species of the genera Neurospora and Fusarium. NX biosynthetic genes and their regulation have been thoroughly investigated in Fusarium fujikuroi, an industrial fungus used for gibberellin production. In this species, carotenoid-overproducing mutants, affected in the regulatory gene carS, exhibit an upregulated expression of the NX pathway. Based on former data on a stimulatory effect of nitrogen starvation on carotenoid biosynthesis, we developed culture conditions with carS mutants allowing the production of deep-pigmented mycelia. With this method, we obtained samples with ca. 8 mg NX/g dry mass, in turn the highest concentration for this carotenoid described so far. NX-rich extracts obtained from these samples were used in parallel with carS-complemented NX-poor extracts obtained under the same conditions, to check the antioxidant properties of this carotenoid in in vitro assays. NX-rich extracts exhibited higher antioxidant capacity than NX-poor extracts, either when considering their quenching activity against [O2(1Δg)] in organic solvent (singlet oxygen absorption capacity (SOAC) assays) or their scavenging activity against different free radicals in aqueous solution and in liposomes. These results make NX a promising carotenoid as a possible feed or food additive, and encourage further studies on its chemical properties.This work was funded by the Spanish Government (Ministerio de Economía y Competitividad, grants BIO2015-69613-R and RTI2018-101902-B-I00; Ministerio de Ciencia, Innovación y Universidades, grants RTI-2018-095131-B-I00 and AGL2015-70218). Authors belong to the Spanish Carotenoid Network, CaRed, (Ministerio de Economía y Competitividad, grant BIO2015-71703-REDT), and the European Carotenoid Network (EuroCaroten) funded by the European Commission COST Action CA15136.Peer reviewedMultidisciplinary Digital Publishing InstituteMinisterio de Economía y Competitividad (España)Ministerio de Ciencia, Innovación y Universidades (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202020202020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/215544reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2015-69613-Rinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-101902-B-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-095131-B-I00info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-70218info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2015-71703-REDThttp://dx.doi.org/10.3390/antiox9060528Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2155442026-05-22T06:33:51Z
dc.title.none.fl_str_mv Neurosporaxanthin Overproduction by Fusarium fujikuroi and Evaluation of Its Antioxidant Properties
title Neurosporaxanthin Overproduction by Fusarium fujikuroi and Evaluation of Its Antioxidant Properties
spellingShingle Neurosporaxanthin Overproduction by Fusarium fujikuroi and Evaluation of Its Antioxidant Properties
Parra-Rivero, Obdulia
Carotenoids
Xanthophylls
Free radicals
Oxidative stress
Fungi
Quenching
Liposomes
title_short Neurosporaxanthin Overproduction by Fusarium fujikuroi and Evaluation of Its Antioxidant Properties
title_full Neurosporaxanthin Overproduction by Fusarium fujikuroi and Evaluation of Its Antioxidant Properties
title_fullStr Neurosporaxanthin Overproduction by Fusarium fujikuroi and Evaluation of Its Antioxidant Properties
title_full_unstemmed Neurosporaxanthin Overproduction by Fusarium fujikuroi and Evaluation of Its Antioxidant Properties
title_sort Neurosporaxanthin Overproduction by Fusarium fujikuroi and Evaluation of Its Antioxidant Properties
dc.creator.none.fl_str_mv Parra-Rivero, Obdulia
Paes-de Barros, Marcelo
Prado, María del Mar
Gil, José Vicente
Hornero-Méndez, Dámaso
Zacarías, Lorenzo
Rodrigo, María Jesús
Limón, M. Carmen
Ávalos, Javier
author Parra-Rivero, Obdulia
author_facet Parra-Rivero, Obdulia
Paes-de Barros, Marcelo
Prado, María del Mar
Gil, José Vicente
Hornero-Méndez, Dámaso
Zacarías, Lorenzo
Rodrigo, María Jesús
Limón, M. Carmen
Ávalos, Javier
author_role author
author2 Paes-de Barros, Marcelo
Prado, María del Mar
Gil, José Vicente
Hornero-Méndez, Dámaso
Zacarías, Lorenzo
Rodrigo, María Jesús
Limón, M. Carmen
Ávalos, Javier
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Ministerio de Ciencia, Innovación y Universidades (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Carotenoids
Xanthophylls
Free radicals
Oxidative stress
Fungi
Quenching
Liposomes
topic Carotenoids
Xanthophylls
Free radicals
Oxidative stress
Fungi
Quenching
Liposomes
description 17 Páginas.-- 5 Figuras
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/215544
url http://hdl.handle.net/10261/215544
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
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info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2015-69613-R
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-101902-B-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-095131-B-I00
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-70218
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2015-71703-REDT
http://dx.doi.org/10.3390/antiox9060528
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
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