Image_3_Biochemical features and modulation of digestive enzymes by environmental temperature in the greater amberjack, Seriola dumerili.tif

1 figure. -- 13% substrate SDS-PAGE showing caseinolysc activity bands in pyloric caeca extracts of individual S. dumerili adapted to different rearing temperatures (18, 22 and 26º C). Get incubation temperature 37º C.

Detalles Bibliográficos
Autores: Navarro-Guillén, Carmen, Yúfera, Manuel, Perera, Erick
Tipo de recurso: conjunto de datos
Fecha de publicación:2022
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/331178
Acceso en línea:http://hdl.handle.net/10261/331178
Access Level:acceso abierto
Palabra clave:Chymotrypsin
Digestive enzymes
Enzyme characterization
Functional flexibility
Isoforms
Seriola dumerili
Water temperature
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spelling Image_3_Biochemical features and modulation of digestive enzymes by environmental temperature in the greater amberjack, Seriola dumerili.tifNavarro-Guillén, CarmenYúfera, ManuelPerera, ErickChymotrypsinDigestive enzymesEnzyme characterizationFunctional flexibilityIsoformsSeriola dumeriliWater temperature1 figure. -- 13% substrate SDS-PAGE showing caseinolysc activity bands in pyloric caeca extracts of individual S. dumerili adapted to different rearing temperatures (18, 22 and 26º C). Get incubation temperature 37º C.The study of fish digestive biochemistry is essential to understand factors that affect the net efficiency of food transformation and growth, and therefore aquaculture profitability. The aim of the present study was to assess the activity and functional characteristics of key digestive enzymes in juveniles of greater amberjack (Seriola dumerili), as well as the possible modulation of their relative importance by water temperature. For that, a combination of biochemical assays and substrate-SDS-PAGE were used. Under physiological conditions pepsin activity was negligible. Chymotrypsin was the most active enzyme in the digestive tract of the greater amberjack, while lipase was the enzyme with lower activity, though both enzymes in addition to trypsin were responsive to water temperature as revealed by discriminant analysis. Seriola dumerili showed to have pH-sensitive and, except for chymotrypsin, thermally robust proteases. Inhibition assays showed the major importance of serine proteases and revealed inverse trypsin and chymotrypsin responses to environmental temperature, with higher trypsin contribution in 26°C-fish while higher chymotrypsin contribution in 18°C-fish. Zymograms revealed three isotrypsin and three isochymotrypsin enzymes, with no variation in the presence of particular isoforms among rearing temperatures. However, they confirmed the role of chymotrypsin activity in providing digestive plasticity, with one of the isoforms being more active at lower temperatures. Thus, results indicate that variation in the relative contribution of chymotrypsin isoenzymes to a particular environmental temperature occurs due to different physic-chemical features of isoforms as a source of functional flexibility. This study assessed for the first time the effects of rearing temperature on greater amberjack digestive enzymes, increasing the knowledge on its digestive biochemistry, and aiding in the improvement of management practices for this species industrialization.Peer reviewedFigshareConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232022info:eu-repo/semantics/datasethttp://purl.org/coar/resource_type/c_ddb1application/pdfhttp://hdl.handle.net/10261/331178reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésNavarro-Guillén, Carmen; Yúfera, Manuel; Perera, Erick. Biochemical features and modulation of digestive enzymes by environmental temperature in the greater amberjack, Seriola dumerili. https://doi.org/10.3389/fmars.2022.960746. http://hdl.handle.net/10261/284524https://doi.org/10.3389/fmars.2022.960746.s003Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3311782026-05-22T06:33:51Z
dc.title.none.fl_str_mv Image_3_Biochemical features and modulation of digestive enzymes by environmental temperature in the greater amberjack, Seriola dumerili.tif
title Image_3_Biochemical features and modulation of digestive enzymes by environmental temperature in the greater amberjack, Seriola dumerili.tif
spellingShingle Image_3_Biochemical features and modulation of digestive enzymes by environmental temperature in the greater amberjack, Seriola dumerili.tif
Navarro-Guillén, Carmen
Chymotrypsin
Digestive enzymes
Enzyme characterization
Functional flexibility
Isoforms
Seriola dumerili
Water temperature
title_short Image_3_Biochemical features and modulation of digestive enzymes by environmental temperature in the greater amberjack, Seriola dumerili.tif
title_full Image_3_Biochemical features and modulation of digestive enzymes by environmental temperature in the greater amberjack, Seriola dumerili.tif
title_fullStr Image_3_Biochemical features and modulation of digestive enzymes by environmental temperature in the greater amberjack, Seriola dumerili.tif
title_full_unstemmed Image_3_Biochemical features and modulation of digestive enzymes by environmental temperature in the greater amberjack, Seriola dumerili.tif
title_sort Image_3_Biochemical features and modulation of digestive enzymes by environmental temperature in the greater amberjack, Seriola dumerili.tif
dc.creator.none.fl_str_mv Navarro-Guillén, Carmen
Yúfera, Manuel
Perera, Erick
author Navarro-Guillén, Carmen
author_facet Navarro-Guillén, Carmen
Yúfera, Manuel
Perera, Erick
author_role author
author2 Yúfera, Manuel
Perera, Erick
author2_role author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Chymotrypsin
Digestive enzymes
Enzyme characterization
Functional flexibility
Isoforms
Seriola dumerili
Water temperature
topic Chymotrypsin
Digestive enzymes
Enzyme characterization
Functional flexibility
Isoforms
Seriola dumerili
Water temperature
description 1 figure. -- 13% substrate SDS-PAGE showing caseinolysc activity bands in pyloric caeca extracts of individual S. dumerili adapted to different rearing temperatures (18, 22 and 26º C). Get incubation temperature 37º C.
publishDate 2022
dc.date.none.fl_str_mv 2022
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/dataset
http://purl.org/coar/resource_type/c_ddb1
format dataset
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/331178
url http://hdl.handle.net/10261/331178
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Navarro-Guillén, Carmen; Yúfera, Manuel; Perera, Erick. Biochemical features and modulation of digestive enzymes by environmental temperature in the greater amberjack, Seriola dumerili. https://doi.org/10.3389/fmars.2022.960746. http://hdl.handle.net/10261/284524
https://doi.org/10.3389/fmars.2022.960746.s003
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Figshare
publisher.none.fl_str_mv Figshare
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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