Effect of cysteamine on cytosolic somatostatin binding-sites in rabbit duodenal mucosa
Administration of cysteamine in rabbits elicited a rapid depletion of both duodenal mucosa and plasma somatostatin. A significant reduction was observed within 5 min, returning toward control values by 150 min. The depletion of somatostatin was associated with an increase in the binding capacity and...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 1987 |
| País: | España |
| Institución: | Universidad de Alcalá (UAH) |
| Repositorio: | e_Buah Biblioteca Digital Universidad de Alcalá |
| Idioma: | inglés |
| OAI Identifier: | oai:ebuah.uah.es:10017/2472 |
| Acceso en línea: | http://hdl.handle.net/10017/2472 https://dx.doi.org/10.1016/0014-4800(87)90061-X |
| Access Level: | acceso abierto |
| Palabra clave: | Somatostatin Rabbit Mucosa Bioquímica Biochemistry Science Ciencia |
| Sumario: | Administration of cysteamine in rabbits elicited a rapid depletion of both duodenal mucosa and plasma somatostatin. A significant reduction was observed within 5 min, returning toward control values by 150 min. The depletion of somatostatin was associated with an increase in the binding capacity and a decrease in the affinity of both high- and low-affinity binding sites present in cytosol of duodenal mucosa. Incubation of cytosolic fraction from control rabbits with 1 mM cysteamine did not modify somatostatin binding. Furthermore, addition of cysteamine at the time of binding assay did not affect the integrity of 125I-Tyr11-somatostatin. It is concluded that in vivo administration of cysteamine to rabbits depletes both duodenal mucosa and plasma somatostatin and leads to up-regulation of duodenal somatostatin binding sites. |
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