Supplementary material of the article Selective brain entry of lipid nanoparticles in haemorrhagic stroke is linked to biphasic blood-brain barrier disruption

15 pages. -- Figure S1: Characterisation data of liposomal formulations used in the study. Figure S2: Assessment of the behaviour function, histological brain changes and weight loss at different time points following induction of ICH model by collagenase injection. -- Figure S3: Histological analys...

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Autores: Al-Ahmady, Zahraa, Dickie, Ben R., Aldred, Isabelle, Jasim, Dhifaf, Barrington, Jack, Haley, Michael J., Lemarchand, Eloise, Coutts, Graham, Kaur, Satinderdeep, Bates, Jessica, Curran, Sarah, Goddard, Rupert, Walker, Megan, Parry-Jones, Adrian, Kostarelos, Kostas
Tipo de recurso: conjunto de datos
Fecha de publicación:2022
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/330298
Acceso en línea:http://hdl.handle.net/10261/330298
Access Level:acceso abierto
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spelling Supplementary material of the article Selective brain entry of lipid nanoparticles in haemorrhagic stroke is linked to biphasic blood-brain barrier disruptionAl-Ahmady, ZahraaDickie, Ben R.Aldred, IsabelleJasim, DhifafBarrington, JackHaley, Michael J.Lemarchand, EloiseCoutts, GrahamKaur, SatinderdeepBates, JessicaCurran, SarahGoddard, RupertWalker, MeganParry-Jones, AdrianKostarelos, Kostas15 pages. -- Figure S1: Characterisation data of liposomal formulations used in the study. Figure S2: Assessment of the behaviour function, histological brain changes and weight loss at different time points following induction of ICH model by collagenase injection. -- Figure S3: Histological analysis of brain tissues of ICH mice at different time points after ICH without injection of DiI-Lp confirmed that minimum autofluorescence signal (yellow signal) was detected in the injury site due to the presence of RBCs in the brain and tissue damage.-- Figure S4: Representative confocal images of (A) ipsilateral and (B) contralateral side of brain tissues after ICH, suggested enhanced Cav1 positive area 3-5 h after ICH (indicated by white arrows) and showed a clear co-localisation with DiI-Lp positive areas. (C) quantification of Cav1 and (D) DiI-Lp positive areas showed a significant increase 3-5 h after ICH which suggest a role of transcytotic transport in the accumulation of liposomes into the lesion site after ICH. -- Figure S5: Representative widefield overview images were obtained at x40 magnification without oil utilising Leica Thunder imager microscope. -- Figure S6 Histological analysis of iron deposition in the n after ICH using H&E and Prussian Blue stain on two different coordinates from the bregma; -0.58 (A&C) and -0.122 (B&D) both in the lesion core (A-B) and lesion rim (C-D). -- Figure S7: Evaluation of DiI-Lp co-localisation with microglia and astrocytes.. -- Figure S8: representative image analysis using Imaris software. -- Supplementary Table 1. Details of MRI scan time points for each mouse.. -- Graphical abstract and schematic graphes were performed using Biorender.Peer reviewedIvyspring International PublisherConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232022info:eu-repo/semantics/datasethttp://purl.org/coar/resource_type/c_ddb1application/pdfhttp://hdl.handle.net/10261/330298reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésAl-Ahmady, Zahraa; Dickie, Ben R.; Aldred, Isabelle; Jasim, Dhifaf; Barrington, Jack; Haley, Michael J.; Lemarchand, Eloise; Coutts, Graham; Kaur, Satinderdeep; Bates, Jessica; Curran, Sarah; Goddard, Rupert; Walker, Megan; Parry-Jones, Adrian; Kostarelos, Kostas. Selective brain entry of lipid nanoparticles in haemorrhagic stroke is linked to biphasic blood-brain barrier disruption. http://doi.org/10.7150/thno.72167. http://hdl.handle.net/10261/280759http://doi.org/10.7150/thno.72167Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3302982026-05-22T06:33:51Z
dc.title.none.fl_str_mv Supplementary material of the article Selective brain entry of lipid nanoparticles in haemorrhagic stroke is linked to biphasic blood-brain barrier disruption
title Supplementary material of the article Selective brain entry of lipid nanoparticles in haemorrhagic stroke is linked to biphasic blood-brain barrier disruption
spellingShingle Supplementary material of the article Selective brain entry of lipid nanoparticles in haemorrhagic stroke is linked to biphasic blood-brain barrier disruption
Al-Ahmady, Zahraa
title_short Supplementary material of the article Selective brain entry of lipid nanoparticles in haemorrhagic stroke is linked to biphasic blood-brain barrier disruption
title_full Supplementary material of the article Selective brain entry of lipid nanoparticles in haemorrhagic stroke is linked to biphasic blood-brain barrier disruption
title_fullStr Supplementary material of the article Selective brain entry of lipid nanoparticles in haemorrhagic stroke is linked to biphasic blood-brain barrier disruption
title_full_unstemmed Supplementary material of the article Selective brain entry of lipid nanoparticles in haemorrhagic stroke is linked to biphasic blood-brain barrier disruption
title_sort Supplementary material of the article Selective brain entry of lipid nanoparticles in haemorrhagic stroke is linked to biphasic blood-brain barrier disruption
dc.creator.none.fl_str_mv Al-Ahmady, Zahraa
Dickie, Ben R.
Aldred, Isabelle
Jasim, Dhifaf
Barrington, Jack
Haley, Michael J.
Lemarchand, Eloise
Coutts, Graham
Kaur, Satinderdeep
Bates, Jessica
Curran, Sarah
Goddard, Rupert
Walker, Megan
Parry-Jones, Adrian
Kostarelos, Kostas
author Al-Ahmady, Zahraa
author_facet Al-Ahmady, Zahraa
Dickie, Ben R.
Aldred, Isabelle
Jasim, Dhifaf
Barrington, Jack
Haley, Michael J.
Lemarchand, Eloise
Coutts, Graham
Kaur, Satinderdeep
Bates, Jessica
Curran, Sarah
Goddard, Rupert
Walker, Megan
Parry-Jones, Adrian
Kostarelos, Kostas
author_role author
author2 Dickie, Ben R.
Aldred, Isabelle
Jasim, Dhifaf
Barrington, Jack
Haley, Michael J.
Lemarchand, Eloise
Coutts, Graham
Kaur, Satinderdeep
Bates, Jessica
Curran, Sarah
Goddard, Rupert
Walker, Megan
Parry-Jones, Adrian
Kostarelos, Kostas
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
description 15 pages. -- Figure S1: Characterisation data of liposomal formulations used in the study. Figure S2: Assessment of the behaviour function, histological brain changes and weight loss at different time points following induction of ICH model by collagenase injection. -- Figure S3: Histological analysis of brain tissues of ICH mice at different time points after ICH without injection of DiI-Lp confirmed that minimum autofluorescence signal (yellow signal) was detected in the injury site due to the presence of RBCs in the brain and tissue damage.-- Figure S4: Representative confocal images of (A) ipsilateral and (B) contralateral side of brain tissues after ICH, suggested enhanced Cav1 positive area 3-5 h after ICH (indicated by white arrows) and showed a clear co-localisation with DiI-Lp positive areas. (C) quantification of Cav1 and (D) DiI-Lp positive areas showed a significant increase 3-5 h after ICH which suggest a role of transcytotic transport in the accumulation of liposomes into the lesion site after ICH. -- Figure S5: Representative widefield overview images were obtained at x40 magnification without oil utilising Leica Thunder imager microscope. -- Figure S6 Histological analysis of iron deposition in the n after ICH using H&E and Prussian Blue stain on two different coordinates from the bregma; -0.58 (A&C) and -0.122 (B&D) both in the lesion core (A-B) and lesion rim (C-D). -- Figure S7: Evaluation of DiI-Lp co-localisation with microglia and astrocytes.. -- Figure S8: representative image analysis using Imaris software. -- Supplementary Table 1. Details of MRI scan time points for each mouse.. -- Graphical abstract and schematic graphes were performed using Biorender.
publishDate 2022
dc.date.none.fl_str_mv 2022
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/dataset
http://purl.org/coar/resource_type/c_ddb1
format dataset
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/330298
url http://hdl.handle.net/10261/330298
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Al-Ahmady, Zahraa; Dickie, Ben R.; Aldred, Isabelle; Jasim, Dhifaf; Barrington, Jack; Haley, Michael J.; Lemarchand, Eloise; Coutts, Graham; Kaur, Satinderdeep; Bates, Jessica; Curran, Sarah; Goddard, Rupert; Walker, Megan; Parry-Jones, Adrian; Kostarelos, Kostas. Selective brain entry of lipid nanoparticles in haemorrhagic stroke is linked to biphasic blood-brain barrier disruption. http://doi.org/10.7150/thno.72167. http://hdl.handle.net/10261/280759
http://doi.org/10.7150/thno.72167
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Ivyspring International Publisher
publisher.none.fl_str_mv Ivyspring International Publisher
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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