Supplementary material of the article Selective brain entry of lipid nanoparticles in haemorrhagic stroke is linked to biphasic blood-brain barrier disruption
15 pages. -- Figure S1: Characterisation data of liposomal formulations used in the study. Figure S2: Assessment of the behaviour function, histological brain changes and weight loss at different time points following induction of ICH model by collagenase injection. -- Figure S3: Histological analys...
| Autores: | , , , , , , , , , , , , , , |
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| Tipo de documento: | conjunto de datos |
| Data de publicação: | 2022 |
| País: | España |
| Recursos: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositório: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/330298 |
| Acesso em linha: | http://hdl.handle.net/10261/330298 |
| Access Level: | Acceso aberto |
| Resumo: | 15 pages. -- Figure S1: Characterisation data of liposomal formulations used in the study. Figure S2: Assessment of the behaviour function, histological brain changes and weight loss at different time points following induction of ICH model by collagenase injection. -- Figure S3: Histological analysis of brain tissues of ICH mice at different time points after ICH without injection of DiI-Lp confirmed that minimum autofluorescence signal (yellow signal) was detected in the injury site due to the presence of RBCs in the brain and tissue damage.-- Figure S4: Representative confocal images of (A) ipsilateral and (B) contralateral side of brain tissues after ICH, suggested enhanced Cav1 positive area 3-5 h after ICH (indicated by white arrows) and showed a clear co-localisation with DiI-Lp positive areas. (C) quantification of Cav1 and (D) DiI-Lp positive areas showed a significant increase 3-5 h after ICH which suggest a role of transcytotic transport in the accumulation of liposomes into the lesion site after ICH. -- Figure S5: Representative widefield overview images were obtained at x40 magnification without oil utilising Leica Thunder imager microscope. -- Figure S6 Histological analysis of iron deposition in the n after ICH using H&E and Prussian Blue stain on two different coordinates from the bregma; -0.58 (A&C) and -0.122 (B&D) both in the lesion core (A-B) and lesion rim (C-D). -- Figure S7: Evaluation of DiI-Lp co-localisation with microglia and astrocytes.. -- Figure S8: representative image analysis using Imaris software. -- Supplementary Table 1. Details of MRI scan time points for each mouse.. -- Graphical abstract and schematic graphes were performed using Biorender. |
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