Sensitive and label-free detection of miRNA-145 by triplex formation

The development of new strategies for detecting microRNAs (miRNAs) has become a crucial step in the diagnostic field. miRNA profiles depend greatly on the sample and the analytical platform employed, leading sometimes to contradictory results. In this work, we study the use of modified parallel tail...

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Autores: Aviñó, Ana|||0000-0003-3047-738X, Huertas, César S.|||0000-0002-3100-4034, Lechuga, Laura M|||0000-0001-5187-5358, Eritja, Ramon|||0000-0001-5383-9334
Tipo de recurso: artículo
Fecha de publicación:2016
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:195664
Acceso en línea:https://ddd.uab.cat/record/195664
https://dx.doi.org/urn:doi:10.1007/s00216-015-9180-6
Access Level:acceso abierto
Palabra clave:8-Amino-2'-deoxyguanosine
Parallel tail-clamp
SPR biosensor
Target miRNA-145
Triplex
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spelling Sensitive and label-free detection of miRNA-145 by triplex formationAviñó, Ana|||0000-0003-3047-738XHuertas, César S.|||0000-0002-3100-4034Lechuga, Laura M|||0000-0001-5187-5358Eritja, Ramon|||0000-0001-5383-93348-Amino-2'-deoxyguanosineParallel tail-clampSPR biosensorTarget miRNA-145TriplexThe development of new strategies for detecting microRNAs (miRNAs) has become a crucial step in the diagnostic field. miRNA profiles depend greatly on the sample and the analytical platform employed, leading sometimes to contradictory results. In this work, we study the use of modified parallel tail-clamps to detect a miRNA sequence involved in tumor suppression by triplex formation. Thermal denaturing curves and circular dichroism (CD) measurements have been performed to confirm that parallel clamps carrying 8-aminoguanine form the most stable triplex structures with their target miRNA. The modified tail-clamps have been tested as bioreceptors in a surface plasmon resonance (SPR) biosensor for the detection of miRNA-145. The detection limit was improved 2.4 times demonstrating that a stable triplex structure is formed between target miRNA and 8-aminoguanine tail-clamp bioreceptor. This new approach is an essential step toward the label-free and reliable detection of miRNA signatures for diagnostic purposes. 22016-01-0120162016-01-01Articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/195664https://dx.doi.org/urn:doi:10.1007/s00216-015-9180-6reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 CTQ2010-20541Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 CTQ2014-52588-RAgència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2009/SGR-208Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2014/SGR-624open accesshttp://purl.org/coar/access_right/c_abf2Aquest material està protegit per drets d'autor i/o drets afins. Podeu utilitzar aquest material en funció del que permet la legislació de drets d'autor i drets afins d'aplicació al vostre cas. Per a d'altres usos heu d'obtenir permís del(s) titular(s) de drets.https://rightsstatements.org/vocab/InC/1.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:1956642026-06-06T12:50:31Z
dc.title.none.fl_str_mv Sensitive and label-free detection of miRNA-145 by triplex formation
title Sensitive and label-free detection of miRNA-145 by triplex formation
spellingShingle Sensitive and label-free detection of miRNA-145 by triplex formation
Aviñó, Ana|||0000-0003-3047-738X
8-Amino-2'-deoxyguanosine
Parallel tail-clamp
SPR biosensor
Target miRNA-145
Triplex
title_short Sensitive and label-free detection of miRNA-145 by triplex formation
title_full Sensitive and label-free detection of miRNA-145 by triplex formation
title_fullStr Sensitive and label-free detection of miRNA-145 by triplex formation
title_full_unstemmed Sensitive and label-free detection of miRNA-145 by triplex formation
title_sort Sensitive and label-free detection of miRNA-145 by triplex formation
dc.creator.none.fl_str_mv Aviñó, Ana|||0000-0003-3047-738X
Huertas, César S.|||0000-0002-3100-4034
Lechuga, Laura M|||0000-0001-5187-5358
Eritja, Ramon|||0000-0001-5383-9334
author Aviñó, Ana|||0000-0003-3047-738X
author_facet Aviñó, Ana|||0000-0003-3047-738X
Huertas, César S.|||0000-0002-3100-4034
Lechuga, Laura M|||0000-0001-5187-5358
Eritja, Ramon|||0000-0001-5383-9334
author_role author
author2 Huertas, César S.|||0000-0002-3100-4034
Lechuga, Laura M|||0000-0001-5187-5358
Eritja, Ramon|||0000-0001-5383-9334
author2_role author
author
author
dc.subject.none.fl_str_mv 8-Amino-2'-deoxyguanosine
Parallel tail-clamp
SPR biosensor
Target miRNA-145
Triplex
topic 8-Amino-2'-deoxyguanosine
Parallel tail-clamp
SPR biosensor
Target miRNA-145
Triplex
description The development of new strategies for detecting microRNAs (miRNAs) has become a crucial step in the diagnostic field. miRNA profiles depend greatly on the sample and the analytical platform employed, leading sometimes to contradictory results. In this work, we study the use of modified parallel tail-clamps to detect a miRNA sequence involved in tumor suppression by triplex formation. Thermal denaturing curves and circular dichroism (CD) measurements have been performed to confirm that parallel clamps carrying 8-aminoguanine form the most stable triplex structures with their target miRNA. The modified tail-clamps have been tested as bioreceptors in a surface plasmon resonance (SPR) biosensor for the detection of miRNA-145. The detection limit was improved 2.4 times demonstrating that a stable triplex structure is formed between target miRNA and 8-aminoguanine tail-clamp bioreceptor. This new approach is an essential step toward the label-free and reliable detection of miRNA signatures for diagnostic purposes.
publishDate 2016
dc.date.none.fl_str_mv 2
2016-01-01
2016
2016-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/195664
https://dx.doi.org/urn:doi:10.1007/s00216-015-9180-6
url https://ddd.uab.cat/record/195664
https://dx.doi.org/urn:doi:10.1007/s00216-015-9180-6
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 CTQ2010-20541
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 CTQ2014-52588-R
Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2009/SGR-208
Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2014/SGR-624
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://rightsstatements.org/vocab/InC/1.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://rightsstatements.org/vocab/InC/1.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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