Role of PRC2-associated factor EPOP in mouse embryonic stem cells differentiation and early development
Polycomb repressive complex 2 (PRC2) is a key epigenetic regulator of gene expression. It acts through its histone-modifying activity, as it is responsible of di- and trimethylating the tail of histone H3 on lysine 27 (H3K27me2/me3), a mark associated with gene repression. PRC2 activity is highly mo...
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| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2019 |
| País: | España |
| Institución: | CBUC, CESCA |
| Repositorio: | TDR. Tesis Doctorales en Red |
| OAI Identifier: | oai:www.tdx.cat:10803/668141 |
| Acceso en línea: | http://hdl.handle.net/10803/668141 |
| Access Level: | acceso abierto |
| Palabra clave: | Polycomb Mouse embryonic stem cells Diferentiation Embrionic development Células madre embrionarias de ratón Diferenciación Desarrollo temprano embrionario 575 |
| Sumario: | Polycomb repressive complex 2 (PRC2) is a key epigenetic regulator of gene expression. It acts through its histone-modifying activity, as it is responsible of di- and trimethylating the tail of histone H3 on lysine 27 (H3K27me2/me3), a mark associated with gene repression. PRC2 activity is highly modulated by a number of sub-stoichiometric factors that allow its recruitment to specific target genes through the interaction with RNA, DNA and histone marks; as well as modulation of its enzymatic activity. EPOP is a recently-identified PRC2-associated factor in mouse embryonic stem cells (mESCs), which is necessary to maintain a low level of expression of PRC2 targets, although the result of this regulation is not yet understood. In this thesis, to further our understanding of the relevance of this EPOP-mediated gene regulation, we investigate the role of EPOP in the context of mESCs differentiation both in vitro and in vivo. |
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