Revisiting the Non-Coding Nature of Pospiviroids

Viroids are small, circular, highly structured pathogens that infect a broad range of plants, causing economic losses. Since their discovery in the 1970s, they have been considered as non-coding pathogens. In the last few years, the discovery of other RNA entities, similar in terms of size and struc...

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Autores: Katsarou, Konstantina, Adkar-Purushothama, Charith Raj, Tassios, Emilios, Samiotaki, Martina, Andronis, Christos, Lisón, Purificación, Nikolaou, Christoforos, Perreault, Jean-Pierre, Kalantidis, Kriton
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/304784
Acceso en línea:http://hdl.handle.net/10261/304784
https://api.elsevier.com/content/abstract/scopus_id/85122885099
Access Level:acceso abierto
Palabra clave:PSTVd
cirRNA
mass spectrometry
non-coding RNA
translation
viroid
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spelling Revisiting the Non-Coding Nature of PospiviroidsKatsarou, KonstantinaAdkar-Purushothama, Charith RajTassios, EmiliosSamiotaki, MartinaAndronis, ChristosLisón, PurificaciónNikolaou, ChristoforosPerreault, Jean-PierreKalantidis, KritonPSTVdcirRNAmass spectrometrynon-coding RNAtranslationviroidViroids are small, circular, highly structured pathogens that infect a broad range of plants, causing economic losses. Since their discovery in the 1970s, they have been considered as non-coding pathogens. In the last few years, the discovery of other RNA entities, similar in terms of size and structure, that were shown to be translated (e.g., cirRNAs, precursors of miRNA, RNA satellites) as well as studies showing that some viroids are located in ribosomes, have reignited the idea that viroids may be translated. In this study, we used advanced bioinformatic analysis, in vitro experiments and LC-MS/MS to search for small viroid peptides of the PSTVd. Our results suggest that in our experimental conditions, even though the circular form of PSTVd is found in ribosomes, no produced peptides were identified. This indicates that the presence of PSTVd in ribosomes is most probably not related to peptide production but rather to another unknown function that requires further study.Peer reviewedConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/304784https://api.elsevier.com/content/abstract/scopus_id/85122885099reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésCellsSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3047842026-05-22T06:33:51Z
dc.title.none.fl_str_mv Revisiting the Non-Coding Nature of Pospiviroids
title Revisiting the Non-Coding Nature of Pospiviroids
spellingShingle Revisiting the Non-Coding Nature of Pospiviroids
Katsarou, Konstantina
PSTVd
cirRNA
mass spectrometry
non-coding RNA
translation
viroid
title_short Revisiting the Non-Coding Nature of Pospiviroids
title_full Revisiting the Non-Coding Nature of Pospiviroids
title_fullStr Revisiting the Non-Coding Nature of Pospiviroids
title_full_unstemmed Revisiting the Non-Coding Nature of Pospiviroids
title_sort Revisiting the Non-Coding Nature of Pospiviroids
dc.creator.none.fl_str_mv Katsarou, Konstantina
Adkar-Purushothama, Charith Raj
Tassios, Emilios
Samiotaki, Martina
Andronis, Christos
Lisón, Purificación
Nikolaou, Christoforos
Perreault, Jean-Pierre
Kalantidis, Kriton
author Katsarou, Konstantina
author_facet Katsarou, Konstantina
Adkar-Purushothama, Charith Raj
Tassios, Emilios
Samiotaki, Martina
Andronis, Christos
Lisón, Purificación
Nikolaou, Christoforos
Perreault, Jean-Pierre
Kalantidis, Kriton
author_role author
author2 Adkar-Purushothama, Charith Raj
Tassios, Emilios
Samiotaki, Martina
Andronis, Christos
Lisón, Purificación
Nikolaou, Christoforos
Perreault, Jean-Pierre
Kalantidis, Kriton
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv PSTVd
cirRNA
mass spectrometry
non-coding RNA
translation
viroid
topic PSTVd
cirRNA
mass spectrometry
non-coding RNA
translation
viroid
description Viroids are small, circular, highly structured pathogens that infect a broad range of plants, causing economic losses. Since their discovery in the 1970s, they have been considered as non-coding pathogens. In the last few years, the discovery of other RNA entities, similar in terms of size and structure, that were shown to be translated (e.g., cirRNAs, precursors of miRNA, RNA satellites) as well as studies showing that some viroids are located in ribosomes, have reignited the idea that viroids may be translated. In this study, we used advanced bioinformatic analysis, in vitro experiments and LC-MS/MS to search for small viroid peptides of the PSTVd. Our results suggest that in our experimental conditions, even though the circular form of PSTVd is found in ribosomes, no produced peptides were identified. This indicates that the presence of PSTVd in ribosomes is most probably not related to peptide production but rather to another unknown function that requires further study.
publishDate 2022
dc.date.none.fl_str_mv 2022
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/304784
https://api.elsevier.com/content/abstract/scopus_id/85122885099
url http://hdl.handle.net/10261/304784
https://api.elsevier.com/content/abstract/scopus_id/85122885099
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Cells
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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