Synthesis and in vitro inhibition properties of siRNA conjugates carrying acridine and quindoline moieties
The synthesis of RNA molecules carrying acridine or quindoline residues at their 3′- and 5′-termini is reported. These conjugates are fully characterized by MALDI-TOF mass spectrometry. Modified siRNA duplexes carrying acridine or quindoline moieties were evaluated for inhibition of the tumor necros...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión aceptada para publicación |
| Fecha de publicación: | 2012 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/125225 |
| Acceso en línea: | http://hdl.handle.net/10261/125225 |
| Access Level: | acceso abierto |
| Palabra clave: | Inhibitors Oligoribonucleotides RNA siRNA Quindoline Acridine Threoninol Tumor necrosis factor |
| Sumario: | The synthesis of RNA molecules carrying acridine or quindoline residues at their 3′- and 5′-termini is reported. These conjugates are fully characterized by MALDI-TOF mass spectrometry. Modified siRNA duplexes carrying acridine or quindoline moieties were evaluated for inhibition of the tumor necrosis factor. The conjugates showed inhibitory properties similar to those of unmodified RNA duplexes in HeLa cells transfected with oligofectamine. The fluorescent properties of acridine derivatives allow direct observation of the cytoplasmatic distribution of modified siRNA inside the cells |
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