Synthesis and structural properties of oligonucleotides covalently linked to acridine and quindoline derivatives through a threoninol linker
Oligonucleotide conjugates containing acridine and quindoline derivatives linked through a threoninol molecule were synthesized. We showed that these conjugates formed duplexes and quadruplexes with higher thermal stability than the corresponding unmodified oligonucleotides. When acridine is located...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2010 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/46951 |
| Acceso en línea: | http://hdl.handle.net/10261/46951 |
| Access Level: | acceso abierto |
| Palabra clave: | Oligonucleotide synthesis DNA duplex DNA-binding drugs DNA Intercalating agents Acridine Quindoline Threoninol Quadruplex Thrombin binding aptamer NMR |
| Sumario: | Oligonucleotide conjugates containing acridine and quindoline derivatives linked through a threoninol molecule were synthesized. We showed that these conjugates formed duplexes and quadruplexes with higher thermal stability than the corresponding unmodified oligonucleotides. When acridine is located in the middle of the sequence, DNA duplexes have a similar stability independently of the natural base present in front of acridine. Self-complementary oligonucleotides and thrombin binding aptamers (TBA) carrying the acridine and quindoline molecules are studied by NMR. |
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