Efficacy of synthetic furanones on listeria monocytogenes biofilm formation

17 pages, 6 figures, 2 tables.-- This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license

Detalles Bibliográficos
Autores: Rodríguez-López, P., Emparanza Barrenengoa, Andrea, Pascual-Sáez, Sergio, López Cabo, Marta
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/197159
Acceso en línea:http://hdl.handle.net/10261/197159
Access Level:acceso abierto
Palabra clave:Biofilms
Food industry
Food safety
furanones
Listeria monocytogenes
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spelling Efficacy of synthetic furanones on listeria monocytogenes biofilm formationRodríguez-López, P.Emparanza Barrenengoa, AndreaPascual-Sáez, SergioLópez Cabo, MartaBiofilmsFood industryFood safetyfuranonesListeria monocytogeneshttp://metadata.un.org/sdg/217 pages, 6 figures, 2 tables.-- This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) licenseFuranones are analogues of acylated homoserine lactones with proven antifouling activity in both Gram-positive and Gram-negative bacteria though the interference of various quorum sensing pathways. In an attempt to find new strategies to prevent and control Listeria monocytogenes biofilm formation on stainless steel (SS) surfaces, different concentrations of six synthetic furanones were applied on biofilms formed by strains isolated from food, environmental, and clinical sources grown onto AISI 316 SS coupons. Among the furanones tested, (Z-)-4-Bromo-5-(bromomethylene)-2(5H)-furanone and 3,4-Dichloro-2(5H)-furanone significantly (p < 0.05) reduced the adhesion capacity (>1 log CFU cm−2) in 24 h treated biofilms. Moreover, individually conducted experiments demonstrated that (Z-)-4-Bromo-5-(bromomethylene)-2(5H)-furanone was able to not only significantly (p < 0.05) prevent L. monocytogenes adhesion but also to reduce the growth rate of planktonic cells up to 48 h in a dose-dependent manner. LIVE/DEAD staining followed by epifluorescence microscopy visualisation confirmed these results show an alteration of the structure of the biofilm in furanone-treated samples. Additionally, it was demonstrated that 20 µmol L−1 of 3,4-Dichloro-2(5H)-furanone dosed at 0, 24 and 96 h was able to maintain a lower level of adhered cells (>1 log CFU cm−2; p < 0.05). Since furanones do not pose a selective pressure on bacteria, these results represent an appealing novel strategy for the prevention of L. monocytogenes biofilm grown onto SS.This research was financially supported by the Spanish Ministerio de Ciencia, Innovación y Universidades [SOLISTA, AGL2016-78549].Peer reviewedMultidisciplinary Digital Publishing InstituteMinisterio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2019201920192019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/197159reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2016-78549-PThe underlying dataset has been published as supplementary material of the article in the publishers platform at DOI https://doi.org/10.3390/foods8120647https://doi.org/10.3390/foods8120647Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1971592026-05-22T06:33:51Z
dc.title.none.fl_str_mv Efficacy of synthetic furanones on listeria monocytogenes biofilm formation
title Efficacy of synthetic furanones on listeria monocytogenes biofilm formation
spellingShingle Efficacy of synthetic furanones on listeria monocytogenes biofilm formation
Rodríguez-López, P.
Biofilms
Food industry
Food safety
furanones
Listeria monocytogenes
http://metadata.un.org/sdg/2
title_short Efficacy of synthetic furanones on listeria monocytogenes biofilm formation
title_full Efficacy of synthetic furanones on listeria monocytogenes biofilm formation
title_fullStr Efficacy of synthetic furanones on listeria monocytogenes biofilm formation
title_full_unstemmed Efficacy of synthetic furanones on listeria monocytogenes biofilm formation
title_sort Efficacy of synthetic furanones on listeria monocytogenes biofilm formation
dc.creator.none.fl_str_mv Rodríguez-López, P.
Emparanza Barrenengoa, Andrea
Pascual-Sáez, Sergio
López Cabo, Marta
author Rodríguez-López, P.
author_facet Rodríguez-López, P.
Emparanza Barrenengoa, Andrea
Pascual-Sáez, Sergio
López Cabo, Marta
author_role author
author2 Emparanza Barrenengoa, Andrea
Pascual-Sáez, Sergio
López Cabo, Marta
author2_role author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Biofilms
Food industry
Food safety
furanones
Listeria monocytogenes
http://metadata.un.org/sdg/2
topic Biofilms
Food industry
Food safety
furanones
Listeria monocytogenes
http://metadata.un.org/sdg/2
description 17 pages, 6 figures, 2 tables.-- This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/197159
url http://hdl.handle.net/10261/197159
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2016-78549-P
The underlying dataset has been published as supplementary material of the article in the publishers platform at DOI https://doi.org/10.3390/foods8120647
https://doi.org/10.3390/foods8120647
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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