Cecal Reduction of Brachyspira and Lesion Severity in Laying Hens Supplemented with Fermented Defatted ‘Alperujo’

Antimicrobial resistance demands the development of therapeutic alternatives such as prebiotics, probiotics, and nutraceuticals. The aim of this study was to assess the antimicrobial proprieties of the nutraceutical fermented defatted “alperujo”, derived from olive oil production, in a laying hen fa...

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Autores: Rebollada Merino, Agustín Miguel, Ugarte Ruiz, María, Gómez-Buendía, Alberto, Bárcena Asensio, María Carmen, García Benzaquén, Nerea, Domínguez Rodríguez, Lucas José, Rodríguez Bertos, Antonio Manuel
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/93232
Acceso en línea:https://hdl.handle.net/20.500.14352/93232
Access Level:acceso abierto
Palabra clave:579
636.09
Brachyspira
Intestinal health
Laying hen
Nutraceutical
Poultry
Microbiología (Veterinaria)
Veterinaria
3109.05 Microbiología
3109 Ciencias Veterinarias
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oai_identifier_str oai:docta.ucm.es:20.500.14352/93232
network_acronym_str ES
network_name_str España
repository_id_str
spelling Cecal Reduction of Brachyspira and Lesion Severity in Laying Hens Supplemented with Fermented Defatted ‘Alperujo’Rebollada Merino, Agustín MiguelUgarte Ruiz, MaríaGómez-Buendía, AlbertoBárcena Asensio, María CarmenGarcía Benzaquén, NereaDomínguez Rodríguez, Lucas JoséRodríguez Bertos, Antonio Manuel579636.09BrachyspiraIntestinal healthLaying henNutraceuticalPoultryMicrobiología (Veterinaria)Veterinaria3109.05 Microbiología3109 Ciencias VeterinariasAntimicrobial resistance demands the development of therapeutic alternatives such as prebiotics, probiotics, and nutraceuticals. The aim of this study was to assess the antimicrobial proprieties of the nutraceutical fermented defatted “alperujo”, derived from olive oil production, in a laying hen farm (n = 122,250) endemic with avian intestinal spirochetosis (Brachyspira spp.). Part of the batch (n = 1440) was divided into six groups of 240 hens each that included 80 or 108-week-old laying hens, supplemented with 0%, 2%, or 6% fermented defatted ‘alperujo’ for a month. At the end of the experiment, eight hens from each group were autopsied and cecal content was subjected to (i) Brachyspira culture and species identification by PCRs, and (ii) direct DNA extraction and Brachyspira qPCR. Furthermore, the ceca were processed for histopathology. Microbiological isolation revealed B. pilosicoli and B. hyodysenteriae co-infection in all groups. The 80-week-old hen group 2% supplemented showed a reduction in the cecal Brachyspira content (qPCR) compared with non-supplemented hens. Cecal histopathology showed a diffuse mild infiltration of lymphocytes, plasma cells, and heterophils; and hyperplasia of the gut-associated lymphoid tissue hyperplasia which decreased in severity in 80-week-old supplemented hens. The reduction in Brachyspira colonization and the severity of the lesions observed in supplemented hens highlights a potential protective function against avian intestinal spirochetosis.MDPIUniversidad Complutense de Madrid20222022-01-0120222022-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/93232reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/932322026-06-02T12:44:21Z
dc.title.none.fl_str_mv Cecal Reduction of Brachyspira and Lesion Severity in Laying Hens Supplemented with Fermented Defatted ‘Alperujo’
title Cecal Reduction of Brachyspira and Lesion Severity in Laying Hens Supplemented with Fermented Defatted ‘Alperujo’
spellingShingle Cecal Reduction of Brachyspira and Lesion Severity in Laying Hens Supplemented with Fermented Defatted ‘Alperujo’
Rebollada Merino, Agustín Miguel
579
636.09
Brachyspira
Intestinal health
Laying hen
Nutraceutical
Poultry
Microbiología (Veterinaria)
Veterinaria
3109.05 Microbiología
3109 Ciencias Veterinarias
title_short Cecal Reduction of Brachyspira and Lesion Severity in Laying Hens Supplemented with Fermented Defatted ‘Alperujo’
title_full Cecal Reduction of Brachyspira and Lesion Severity in Laying Hens Supplemented with Fermented Defatted ‘Alperujo’
title_fullStr Cecal Reduction of Brachyspira and Lesion Severity in Laying Hens Supplemented with Fermented Defatted ‘Alperujo’
title_full_unstemmed Cecal Reduction of Brachyspira and Lesion Severity in Laying Hens Supplemented with Fermented Defatted ‘Alperujo’
title_sort Cecal Reduction of Brachyspira and Lesion Severity in Laying Hens Supplemented with Fermented Defatted ‘Alperujo’
dc.creator.none.fl_str_mv Rebollada Merino, Agustín Miguel
Ugarte Ruiz, María
Gómez-Buendía, Alberto
Bárcena Asensio, María Carmen
García Benzaquén, Nerea
Domínguez Rodríguez, Lucas José
Rodríguez Bertos, Antonio Manuel
author Rebollada Merino, Agustín Miguel
author_facet Rebollada Merino, Agustín Miguel
Ugarte Ruiz, María
Gómez-Buendía, Alberto
Bárcena Asensio, María Carmen
García Benzaquén, Nerea
Domínguez Rodríguez, Lucas José
Rodríguez Bertos, Antonio Manuel
author_role author
author2 Ugarte Ruiz, María
Gómez-Buendía, Alberto
Bárcena Asensio, María Carmen
García Benzaquén, Nerea
Domínguez Rodríguez, Lucas José
Rodríguez Bertos, Antonio Manuel
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 579
636.09
Brachyspira
Intestinal health
Laying hen
Nutraceutical
Poultry
Microbiología (Veterinaria)
Veterinaria
3109.05 Microbiología
3109 Ciencias Veterinarias
topic 579
636.09
Brachyspira
Intestinal health
Laying hen
Nutraceutical
Poultry
Microbiología (Veterinaria)
Veterinaria
3109.05 Microbiología
3109 Ciencias Veterinarias
description Antimicrobial resistance demands the development of therapeutic alternatives such as prebiotics, probiotics, and nutraceuticals. The aim of this study was to assess the antimicrobial proprieties of the nutraceutical fermented defatted “alperujo”, derived from olive oil production, in a laying hen farm (n = 122,250) endemic with avian intestinal spirochetosis (Brachyspira spp.). Part of the batch (n = 1440) was divided into six groups of 240 hens each that included 80 or 108-week-old laying hens, supplemented with 0%, 2%, or 6% fermented defatted ‘alperujo’ for a month. At the end of the experiment, eight hens from each group were autopsied and cecal content was subjected to (i) Brachyspira culture and species identification by PCRs, and (ii) direct DNA extraction and Brachyspira qPCR. Furthermore, the ceca were processed for histopathology. Microbiological isolation revealed B. pilosicoli and B. hyodysenteriae co-infection in all groups. The 80-week-old hen group 2% supplemented showed a reduction in the cecal Brachyspira content (qPCR) compared with non-supplemented hens. Cecal histopathology showed a diffuse mild infiltration of lymphocytes, plasma cells, and heterophils; and hyperplasia of the gut-associated lymphoid tissue hyperplasia which decreased in severity in 80-week-old supplemented hens. The reduction in Brachyspira colonization and the severity of the lesions observed in supplemented hens highlights a potential protective function against avian intestinal spirochetosis.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-01-01
2022
2022-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/93232
url https://hdl.handle.net/20.500.14352/93232
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300724

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