Single mechanosensitive and ca(2+)-sensitive channel currents recorded from mouse and human embryonic stem cells

Cell-attached and inside-out patch clamp recording was used to compare the functional expression of membrane ion channels in mouse and human embryonic stem cells (ESCs). Both ESCs express mechanosensitive Ca(2+) permeant cation channels (MscCa) and large conductance (200 pS) Ca(2+)-sensitive K(+) (B...

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Autores: Soria Escoms, Bernat, Navas, Sergio, Hmadcha, Abdelkrim, Hamill, Owen P.
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2012
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/123162
Acceso en línea:http://hdl.handle.net/10261/123162
Access Level:acceso abierto
Palabra clave:Calcium sensitive channels
Embryonic stem cells (ESCs)
Mechanosensitive channels
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spelling Single mechanosensitive and ca(2+)-sensitive channel currents recorded from mouse and human embryonic stem cellsSoria Escoms, BernatNavas, SergioHmadcha, AbdelkrimHamill, Owen P.Calcium sensitive channelsEmbryonic stem cells (ESCs)Mechanosensitive channelsCell-attached and inside-out patch clamp recording was used to compare the functional expression of membrane ion channels in mouse and human embryonic stem cells (ESCs). Both ESCs express mechanosensitive Ca(2+) permeant cation channels (MscCa) and large conductance (200 pS) Ca(2+)-sensitive K(+) (BK(Ca2+)) channels but with markedly different patch densities. MscCa is expressed at higher density in mESCs compared with hESCs (70 % vs. 3 % of patches), whereas the BK(Ca2+) channel is more highly expressed in hESCs compared with mESCs (~50 % vs. 1 % of patches). ESCs of both species express a smaller conductance (25 pS) nonselective cation channel that is activated upon inside-out patch formation but is neither mechanosensitive nor strictly Ca(2+)-dependent. The finding that mouse and human ESCs express different channels that sense membrane tension and intracellular [Ca(2+)] may contribute to their different patterns of growth and differentiation in response to mechanical and chemical cues.OH was supported by a travel/stay Grant from Ministerio de Educación y Ciencia (SAB2006-0211) and in the United States by grants from the National Cancer Institute and the Department of Defense. BS and AH are supported by the Fundación Progreso y Salud, Consejería de Salud, Junta de Andalucía (PI-0022/ 2008); Consejería de Innovación Ciencia y Empresa, Junta de Andalucía (CTS-6505; INP-2011-1615-900000); FEDER cofunded grants from Instituto de Salud Carlos III (Red TerCel-RD06/0010/0025; PI10/00964), and the Ministry of Health and Consumer Affairs (Advanced Therapies Program TRA-120). CIBERDEM is an initiative of the Instituto de Salud Carlos III.Peer ReviewedSpringer NatureMinisterio de Educación y Ciencia (España)National Cancer Institute (US)Department of Defense (US)Fundación Progreso y SaludJunta de AndalucíaEuropean CommissionInstituto de Salud Carlos IIIMinisterio de Sanidad y Consumo (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2015201520122015info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/123162reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1007/s00232-012-9523-6Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1231622026-05-22T06:33:51Z
dc.title.none.fl_str_mv Single mechanosensitive and ca(2+)-sensitive channel currents recorded from mouse and human embryonic stem cells
title Single mechanosensitive and ca(2+)-sensitive channel currents recorded from mouse and human embryonic stem cells
spellingShingle Single mechanosensitive and ca(2+)-sensitive channel currents recorded from mouse and human embryonic stem cells
Soria Escoms, Bernat
Calcium sensitive channels
Embryonic stem cells (ESCs)
Mechanosensitive channels
title_short Single mechanosensitive and ca(2+)-sensitive channel currents recorded from mouse and human embryonic stem cells
title_full Single mechanosensitive and ca(2+)-sensitive channel currents recorded from mouse and human embryonic stem cells
title_fullStr Single mechanosensitive and ca(2+)-sensitive channel currents recorded from mouse and human embryonic stem cells
title_full_unstemmed Single mechanosensitive and ca(2+)-sensitive channel currents recorded from mouse and human embryonic stem cells
title_sort Single mechanosensitive and ca(2+)-sensitive channel currents recorded from mouse and human embryonic stem cells
dc.creator.none.fl_str_mv Soria Escoms, Bernat
Navas, Sergio
Hmadcha, Abdelkrim
Hamill, Owen P.
author Soria Escoms, Bernat
author_facet Soria Escoms, Bernat
Navas, Sergio
Hmadcha, Abdelkrim
Hamill, Owen P.
author_role author
author2 Navas, Sergio
Hmadcha, Abdelkrim
Hamill, Owen P.
author2_role author
author
author
dc.contributor.none.fl_str_mv Ministerio de Educación y Ciencia (España)
National Cancer Institute (US)
Department of Defense (US)
Fundación Progreso y Salud
Junta de Andalucía
European Commission
Instituto de Salud Carlos III
Ministerio de Sanidad y Consumo (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Calcium sensitive channels
Embryonic stem cells (ESCs)
Mechanosensitive channels
topic Calcium sensitive channels
Embryonic stem cells (ESCs)
Mechanosensitive channels
description Cell-attached and inside-out patch clamp recording was used to compare the functional expression of membrane ion channels in mouse and human embryonic stem cells (ESCs). Both ESCs express mechanosensitive Ca(2+) permeant cation channels (MscCa) and large conductance (200 pS) Ca(2+)-sensitive K(+) (BK(Ca2+)) channels but with markedly different patch densities. MscCa is expressed at higher density in mESCs compared with hESCs (70 % vs. 3 % of patches), whereas the BK(Ca2+) channel is more highly expressed in hESCs compared with mESCs (~50 % vs. 1 % of patches). ESCs of both species express a smaller conductance (25 pS) nonselective cation channel that is activated upon inside-out patch formation but is neither mechanosensitive nor strictly Ca(2+)-dependent. The finding that mouse and human ESCs express different channels that sense membrane tension and intracellular [Ca(2+)] may contribute to their different patterns of growth and differentiation in response to mechanical and chemical cues.
publishDate 2012
dc.date.none.fl_str_mv 2012
2015
2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/123162
url http://hdl.handle.net/10261/123162
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1007/s00232-012-9523-6
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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