Single mechanosensitive and ca(2+)-sensitive channel currents recorded from mouse and human embryonic stem cells
Cell-attached and inside-out patch clamp recording was used to compare the functional expression of membrane ion channels in mouse and human embryonic stem cells (ESCs). Both ESCs express mechanosensitive Ca(2+) permeant cation channels (MscCa) and large conductance (200 pS) Ca(2+)-sensitive K(+) (B...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión aceptada para publicación |
| Fecha de publicación: | 2012 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/123162 |
| Acceso en línea: | http://hdl.handle.net/10261/123162 |
| Access Level: | acceso abierto |
| Palabra clave: | Calcium sensitive channels Embryonic stem cells (ESCs) Mechanosensitive channels |
| Sumario: | Cell-attached and inside-out patch clamp recording was used to compare the functional expression of membrane ion channels in mouse and human embryonic stem cells (ESCs). Both ESCs express mechanosensitive Ca(2+) permeant cation channels (MscCa) and large conductance (200 pS) Ca(2+)-sensitive K(+) (BK(Ca2+)) channels but with markedly different patch densities. MscCa is expressed at higher density in mESCs compared with hESCs (70 % vs. 3 % of patches), whereas the BK(Ca2+) channel is more highly expressed in hESCs compared with mESCs (~50 % vs. 1 % of patches). ESCs of both species express a smaller conductance (25 pS) nonselective cation channel that is activated upon inside-out patch formation but is neither mechanosensitive nor strictly Ca(2+)-dependent. The finding that mouse and human ESCs express different channels that sense membrane tension and intracellular [Ca(2+)] may contribute to their different patterns of growth and differentiation in response to mechanical and chemical cues. |
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