Effect of lipid supplementation on the endogenous synthesis of milk cis-9 trans-11 CLA in dairy sheep and goats. A tracer assay with 13C-vaccenic acid

14 páginas, 2 figuras, 4 tablas.

Detalles Bibliográficos
Autores: Toral, Pablo G., Hervás, Gonzalo, Frutos, Pilar
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/256939
Acceso en línea:http://hdl.handle.net/10261/256939
Access Level:acceso abierto
Palabra clave:Caprine
Desaturation
Fatty acid
Ovine
Stable isotope
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repository_id_str
dc.title.none.fl_str_mv Effect of lipid supplementation on the endogenous synthesis of milk cis-9 trans-11 CLA in dairy sheep and goats. A tracer assay with 13C-vaccenic acid
title Effect of lipid supplementation on the endogenous synthesis of milk cis-9 trans-11 CLA in dairy sheep and goats. A tracer assay with 13C-vaccenic acid
spellingShingle Effect of lipid supplementation on the endogenous synthesis of milk cis-9 trans-11 CLA in dairy sheep and goats. A tracer assay with 13C-vaccenic acid
Toral, Pablo G.
Caprine
Desaturation
Fatty acid
Ovine
Stable isotope
title_short Effect of lipid supplementation on the endogenous synthesis of milk cis-9 trans-11 CLA in dairy sheep and goats. A tracer assay with 13C-vaccenic acid
title_full Effect of lipid supplementation on the endogenous synthesis of milk cis-9 trans-11 CLA in dairy sheep and goats. A tracer assay with 13C-vaccenic acid
title_fullStr Effect of lipid supplementation on the endogenous synthesis of milk cis-9 trans-11 CLA in dairy sheep and goats. A tracer assay with 13C-vaccenic acid
title_full_unstemmed Effect of lipid supplementation on the endogenous synthesis of milk cis-9 trans-11 CLA in dairy sheep and goats. A tracer assay with 13C-vaccenic acid
title_sort Effect of lipid supplementation on the endogenous synthesis of milk cis-9 trans-11 CLA in dairy sheep and goats. A tracer assay with 13C-vaccenic acid
dc.creator.none.fl_str_mv Toral, Pablo G.
Hervás, Gonzalo
Frutos, Pilar
author Toral, Pablo G.
author_facet Toral, Pablo G.
Hervás, Gonzalo
Frutos, Pilar
author_role author
author2 Hervás, Gonzalo
Frutos, Pilar
author2_role author
author
dc.contributor.none.fl_str_mv Agencia Estatal de Investigación (España)
European Commission
Consejo Superior de Investigaciones Científicas (España)
Ministerio de Economía y Competitividad (España)
Toral, Pablo G. [0000-0002-1913-7707]
Hervás, Gonzalo [0000-0002-0013-7459]
Frutos, Pilar [0000-0002-4919-5094]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Caprine
Desaturation
Fatty acid
Ovine
Stable isotope
topic Caprine
Desaturation
Fatty acid
Ovine
Stable isotope
description 14 páginas, 2 figuras, 4 tablas.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/256939
url http://hdl.handle.net/10261/256939
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
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info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-87812-R
info:eu-repo/grantAgreement/MINECO//RYC-2015-17230
Toral, Pablo G.; Hervás, Gonzalo; Frutos, Pilar; 2021; Dataset: Effect of lipid supplementation on the endogenous synthesis of milk cis-9,trans-11 CLA in dairy sheep and goats. A tracer assay with 13C-vaccenic acid [Dataset]; DIGITAL.CSIC;http://dx.doi.org/10.20350/digitalCSIC/13862
https://dx.doi.org/10.3168/jds.2021-20728
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
American Dairy Science Association
publisher.none.fl_str_mv Elsevier
American Dairy Science Association
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling Effect of lipid supplementation on the endogenous synthesis of milk cis-9 trans-11 CLA in dairy sheep and goats. A tracer assay with 13C-vaccenic acidToral, Pablo G.Hervás, GonzaloFrutos, PilarCaprineDesaturationFatty acidOvineStable isotope14 páginas, 2 figuras, 4 tablas.A major proportion of milk rumenic acid (RA; cis9,trans-11 CLA) is synthesized through mammary Δ9 -desaturation of vaccenic acid (VA; trans-11 18:1). Diet composition may determine the relative contribution of this endogenous synthesis to milk RA content, with effects that might differ between ruminant species. However, this hypothesis is mostly based on estimated values, proxies of stearoyl-CoA desaturase (SCD) activity, and indirect comparisons between publications in the literature. With the aim of providing new insights into this issue, in vivo Δ9 -desaturation of 13C-labeled VA (measured via milk 13C-VA and -RA secretion) was directly compared in sheep and goats fed a diet without lipid supplementation or including 2% of linseed oil. Four Assaf sheep and 4 Murciano-Granadina goats were used in a replicated 2 × 2 crossover design to test the effects of the 2 dietary treatments during 2 consecutive 25-d periods. On d 22 of each period, 500 mg of 13C-VA were i.v. injected to each animal. Dairy performance, milk fatty acid profile, including isotope analysis, and mammary mRNA abundance of genes coding for SCD were examined on d 21 to 25 of each period. Supplementation with linseed oil improved milk fat concentration and increased the content of milk VA and RA. However, the isotopic tracer assay suggested no variation in the relative proportion of VA desaturated to milk RA, and the percentage of this CLA isomer deriving from SCD activity would remain constant regardless of dietary treatment. These results put into question a major effect of lipid supplementation on the endogenous synthesis of milk RA and support that mammary Δ9 -desaturation capacity would not represent a limiting factor when designing feeding strategies to increase milk RA content. The lack of dietinduced effects was common to caprines and ovines, but inherent interspecies differences in mammary lipogenesis were found. Thus, the higher proportions of VA desaturation and endogenous synthesis of milk RA in sheep supported a greater SCD activity compared with goats, a finding that was not associated with the similar mRNA abundance of SCD1 in the 2 species. On the other hand, transfer efficiency of the isotopic tracer to milk was 37% higher in caprine than in ovine, suggesting a greater efficiency in mammary fatty acid uptake from plasma in caprine.This work was supported by the Spanish Research State Agency (Agencia Estatal de Investigación, AEI), by the European Regional Development Fund (project AGL2017-87812-R, AEI/FEDER, EU), and by the Spanish National Research Council (CSIC; PIE 202040E100). P. G. Toral benefited from a Ramón y Cajal research contract (RYC-2015-17230), from the Spanish Ministry of Economy and Competitiveness (MINECO), co-funded by the European Social Fund. The authors thank V. Peiró from the Stable Isotopes Laboratory of the Interdepartmental Research Service (SIdI, Autonomous University of Madrid, Spain) for her assistance with isotope analysis. The authors have not stated any conflicts of interest.Peer reviewedElsevierAmerican Dairy Science AssociationAgencia Estatal de Investigación (España)European CommissionConsejo Superior de Investigaciones Científicas (España)Ministerio de Economía y Competitividad (España)Toral, Pablo G. [0000-0002-1913-7707]Hervás, Gonzalo [0000-0002-0013-7459]Frutos, Pilar [0000-0002-4919-5094]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202120212022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/256939reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-87812-Rinfo:eu-repo/grantAgreement/MINECO//RYC-2015-17230Toral, Pablo G.; Hervás, Gonzalo; Frutos, Pilar; 2021; Dataset: Effect of lipid supplementation on the endogenous synthesis of milk cis-9,trans-11 CLA in dairy sheep and goats. A tracer assay with 13C-vaccenic acid [Dataset]; DIGITAL.CSIC;http://dx.doi.org/10.20350/digitalCSIC/13862https://dx.doi.org/10.3168/jds.2021-20728Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2569392026-05-22T06:33:51Z
score 15,811543

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