A novel checkpoint pathway controls actomyosin ring constriction trigger in fission yeast

[EN]In fission yeast, the septation initiation network (SIN) ensures temporal coordination between actomyosin ring (CAR) constriction with membrane ingression and septum synthesis. However, questions remain about CAR regulation under stress conditions. We show that Rgf1p (Rho1p GEF), participates in...

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Detalles Bibliográficos
Autores: Edreira González, Tomás, Celador Albarrán , Rubén, Manjón Pérez, Elvira, Sánchez Martín, Yolanda
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Universidad de Salamanca (USAL)
Repositorio:GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:oai:gredos.usal.es:10366/169492
Acceso en línea:http://hdl.handle.net/10366/169492
Access Level:acceso abierto
Palabra clave:Schizosaccharomyces pombe
Fission yeast
Actomyosin ring
Septation initiation network
Rgf1p
2407 Biología Celular
2414 Microbiología
2415 Biología Molecular
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repository_id_str
spelling A novel checkpoint pathway controls actomyosin ring constriction trigger in fission yeastEdreira González, TomásCelador Albarrán , RubénManjón Pérez, ElviraSánchez Martín, YolandaSchizosaccharomyces pombeFission yeastActomyosin ringSeptation initiation networkRgf1p2407 Biología Celular2414 Microbiología2415 Biología Molecular[EN]In fission yeast, the septation initiation network (SIN) ensures temporal coordination between actomyosin ring (CAR) constriction with membrane ingression and septum synthesis. However, questions remain about CAR regulation under stress conditions. We show that Rgf1p (Rho1p GEF), participates in a delay of cytokinesis under cell wall stress (blankophor, BP). BP did not interfere with CAR assembly or the rate of CAR constriction, but did delay the onset of constriction in the wild type cells but not in the rgf1D cells. This delay was also abolished in the absence of Pmk1p, the MAPK of the cell integrity pathway (CIP), leading to premature abscission and a multi-septated phenotype. Moreover, cytokinesis delay correlates with maintained SIN signaling and depends on the SIN to be achieved. Thus, we propose that the CIP participates in a checkpoint, capable of triggering a CAR constriction delay through the SIN pathway to ensure that cytokinesis terminates successfully.MEIC, Spain (BFU2017-84508-P) Regional Government of Castile and Leon (SA073U14) Regional Government of Castile and Leon & The European Social Fund University of Salamanca202620262020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10366/169492reponame:GREDOS. Repositorio Institucional de la Universidad de Salamancainstname:Universidad de Salamanca (USAL)InglésBFU2017-84508-PSA073U14Attribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:gredos.usal.es:10366/1694922026-06-07T06:28:51Z
dc.title.none.fl_str_mv A novel checkpoint pathway controls actomyosin ring constriction trigger in fission yeast
title A novel checkpoint pathway controls actomyosin ring constriction trigger in fission yeast
spellingShingle A novel checkpoint pathway controls actomyosin ring constriction trigger in fission yeast
Edreira González, Tomás
Schizosaccharomyces pombe
Fission yeast
Actomyosin ring
Septation initiation network
Rgf1p
2407 Biología Celular
2414 Microbiología
2415 Biología Molecular
title_short A novel checkpoint pathway controls actomyosin ring constriction trigger in fission yeast
title_full A novel checkpoint pathway controls actomyosin ring constriction trigger in fission yeast
title_fullStr A novel checkpoint pathway controls actomyosin ring constriction trigger in fission yeast
title_full_unstemmed A novel checkpoint pathway controls actomyosin ring constriction trigger in fission yeast
title_sort A novel checkpoint pathway controls actomyosin ring constriction trigger in fission yeast
dc.creator.none.fl_str_mv Edreira González, Tomás
Celador Albarrán , Rubén
Manjón Pérez, Elvira
Sánchez Martín, Yolanda
author Edreira González, Tomás
author_facet Edreira González, Tomás
Celador Albarrán , Rubén
Manjón Pérez, Elvira
Sánchez Martín, Yolanda
author_role author
author2 Celador Albarrán , Rubén
Manjón Pérez, Elvira
Sánchez Martín, Yolanda
author2_role author
author
author
dc.subject.none.fl_str_mv Schizosaccharomyces pombe
Fission yeast
Actomyosin ring
Septation initiation network
Rgf1p
2407 Biología Celular
2414 Microbiología
2415 Biología Molecular
topic Schizosaccharomyces pombe
Fission yeast
Actomyosin ring
Septation initiation network
Rgf1p
2407 Biología Celular
2414 Microbiología
2415 Biología Molecular
description [EN]In fission yeast, the septation initiation network (SIN) ensures temporal coordination between actomyosin ring (CAR) constriction with membrane ingression and septum synthesis. However, questions remain about CAR regulation under stress conditions. We show that Rgf1p (Rho1p GEF), participates in a delay of cytokinesis under cell wall stress (blankophor, BP). BP did not interfere with CAR assembly or the rate of CAR constriction, but did delay the onset of constriction in the wild type cells but not in the rgf1D cells. This delay was also abolished in the absence of Pmk1p, the MAPK of the cell integrity pathway (CIP), leading to premature abscission and a multi-septated phenotype. Moreover, cytokinesis delay correlates with maintained SIN signaling and depends on the SIN to be achieved. Thus, we propose that the CIP participates in a checkpoint, capable of triggering a CAR constriction delay through the SIN pathway to ensure that cytokinesis terminates successfully.
publishDate 2020
dc.date.none.fl_str_mv 2020
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10366/169492
url http://hdl.handle.net/10366/169492
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv BFU2017-84508-P
SA073U14
dc.rights.none.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:GREDOS. Repositorio Institucional de la Universidad de Salamanca
instname:Universidad de Salamanca (USAL)
instname_str Universidad de Salamanca (USAL)
reponame_str GREDOS. Repositorio Institucional de la Universidad de Salamanca
collection GREDOS. Repositorio Institucional de la Universidad de Salamanca
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869413810575507456
score 15,812429

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