Importin α and vNEBD Control Meiotic Spindle Disassembly in Fission Yeast.

In metazoans, the nuclear envelope (NE) breakdown (NEBD) occurs during "open" mitosis and meiosis. In the fission yeast Schizosaccharomyces pombe, the mitosis and the first meiotic division (MI) are "closed," during which the NE is maintained. Intriguingly, during the second meio...

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Detalhes bibliográficos
Autores: Daga, Rafael, Salas-Pino, Silvia, Flor-Parra, Ignacio, Iglesias Romero, Ana Belén, Lucena Rafael, Jiménez, Juan
Formato: artículo
Fecha de publicación:2018
País:España
Recursos:Universidad Pablo de Olavide (UPO)
Repositorio:RIO. Repositorio Institucional Olavide
Idioma:inglés
OAI Identifier:oai:rio.upo.es:10433/25242
Acesso em linha:https://hdl.handle.net/10433/25242
Access Level:acceso abierto
Palavra-chave:Schizosaccharomyces pombe
Fission yeast
Nucleocytoplasmic transport
Imp1
vNEBD
Nuclear envelope
Meiosis
Spindle disassembly
Descrição
Resumo:In metazoans, the nuclear envelope (NE) breakdown (NEBD) occurs during "open" mitosis and meiosis. In the fission yeast Schizosaccharomyces pombe, the mitosis and the first meiotic division (MI) are "closed," during which the NE is maintained. Intriguingly, during the second meiotic division (MII), the NE is also maintained, but nuclear and cytoplasmic molecules are mixed similarly to open mitosis, a phenomenon of unknown biological significance called "virtual" NEBD (vNEBD). Here, we show that importin-α-dependent nucleocytoplasmic transport regulates spindle disassembly late in anaphase B at MI, as previously reported for mitosis. At MII, however, spindle dissolution is triggered by vNEBD early in anaphase B, a mechanism that short-circuits the nucleocytoplasmic transport system. We demonstrate that the sequential action of these two spindle disassembly systems regulates the spatiotemporal order and ploidy of the meiotic products.