Validation of a gas chromatography-mass spectrometry method for the analysis of sterol oxidation products in serum

A validated gas chromatography-mass spectrometry (GC-MS) detection method for the quantitative analysis of sterol oxidation products (SOPs) in serum is described. After a lipid extraction procedure with chloroform-methanol, a cold saponification and purification by solid phase extraction, oxysterols...

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Authors: Menendez-Carreño, M. (María)|||/items/3cc43ac0-63f9-4e93-8647-26c827458a6b, Garcia-Herreros, C. (Cecilia)|||/items/c016d2bc-9dd8-41ce-8cf4-938234ba9f55, Ansorena-Artieda, D. (Diana)|||/items/3ac83dfe-b2e3-4a2d-a7e4-2813388afc29, Astiasarán, I. (Iciar)|||/items/34b867c2-957e-4a1f-8a1d-c0a6a8e6b70b
Format: article
Publication Date:2008
Country:España
Institution:Universidad de Navarra
Repository:Dadun. Depósito Académico Digital de la Universidad de Navarra
Language:English
OAI Identifier:oai:dadun.unav.edu:10171/22124
Online Access:https://hdl.handle.net/10171/22124
Access Level:Open access
Keyword:Serum
Sterols
COPs
Linearity
Recovery
Repeatability
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spelling Validation of a gas chromatography-mass spectrometry method for the analysis of sterol oxidation products in serumMenendez-Carreño, M. (María)|||/items/3cc43ac0-63f9-4e93-8647-26c827458a6bGarcia-Herreros, C. (Cecilia)|||/items/c016d2bc-9dd8-41ce-8cf4-938234ba9f55Ansorena-Artieda, D. (Diana)|||/items/3ac83dfe-b2e3-4a2d-a7e4-2813388afc29Astiasarán, I. (Iciar)|||/items/34b867c2-957e-4a1f-8a1d-c0a6a8e6b70bSerumSterolsCOPsLinearityRecoveryRepeatabilityA validated gas chromatography-mass spectrometry (GC-MS) detection method for the quantitative analysis of sterol oxidation products (SOPs) in serum is described. After a lipid extraction procedure with chloroform-methanol, a cold saponification and purification by solid phase extraction, oxysterols were derivatized to form trimethyl-sylil-ethers which were subjected to GC-MS analysis. Calibration curves for cholesterol oxidation products showed determination coefficient (R(2)) of 1.0, with low values for the coefficient of variation of the response factors (< 1%). Detection and quantification limits were below 5 ng/mL and 10 ng/mL, respectively. Recovery data were between 77.65% and 110.29% (CV < 10% for all compounds). Good results were obtained for within- and between-day repeatability, with values below 10%. In conclusion, the method performed is suitable for the determination and quantification of SOPs in serum.ElsevierDadun. Depósito Académico Digital Universidad de Navarra20122012-05-1820082008-01-0120082008-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/22124reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/221242026-06-21T12:47:57Z
dc.title.none.fl_str_mv Validation of a gas chromatography-mass spectrometry method for the analysis of sterol oxidation products in serum
title Validation of a gas chromatography-mass spectrometry method for the analysis of sterol oxidation products in serum
spellingShingle Validation of a gas chromatography-mass spectrometry method for the analysis of sterol oxidation products in serum
Menendez-Carreño, M. (María)|||/items/3cc43ac0-63f9-4e93-8647-26c827458a6b
Serum
Sterols
COPs
Linearity
Recovery
Repeatability
title_short Validation of a gas chromatography-mass spectrometry method for the analysis of sterol oxidation products in serum
title_full Validation of a gas chromatography-mass spectrometry method for the analysis of sterol oxidation products in serum
title_fullStr Validation of a gas chromatography-mass spectrometry method for the analysis of sterol oxidation products in serum
title_full_unstemmed Validation of a gas chromatography-mass spectrometry method for the analysis of sterol oxidation products in serum
title_sort Validation of a gas chromatography-mass spectrometry method for the analysis of sterol oxidation products in serum
dc.creator.none.fl_str_mv Menendez-Carreño, M. (María)|||/items/3cc43ac0-63f9-4e93-8647-26c827458a6b
Garcia-Herreros, C. (Cecilia)|||/items/c016d2bc-9dd8-41ce-8cf4-938234ba9f55
Ansorena-Artieda, D. (Diana)|||/items/3ac83dfe-b2e3-4a2d-a7e4-2813388afc29
Astiasarán, I. (Iciar)|||/items/34b867c2-957e-4a1f-8a1d-c0a6a8e6b70b
author Menendez-Carreño, M. (María)|||/items/3cc43ac0-63f9-4e93-8647-26c827458a6b
author_facet Menendez-Carreño, M. (María)|||/items/3cc43ac0-63f9-4e93-8647-26c827458a6b
Garcia-Herreros, C. (Cecilia)|||/items/c016d2bc-9dd8-41ce-8cf4-938234ba9f55
Ansorena-Artieda, D. (Diana)|||/items/3ac83dfe-b2e3-4a2d-a7e4-2813388afc29
Astiasarán, I. (Iciar)|||/items/34b867c2-957e-4a1f-8a1d-c0a6a8e6b70b
author_role author
author2 Garcia-Herreros, C. (Cecilia)|||/items/c016d2bc-9dd8-41ce-8cf4-938234ba9f55
Ansorena-Artieda, D. (Diana)|||/items/3ac83dfe-b2e3-4a2d-a7e4-2813388afc29
Astiasarán, I. (Iciar)|||/items/34b867c2-957e-4a1f-8a1d-c0a6a8e6b70b
author2_role author
author
author
dc.contributor.none.fl_str_mv Dadun. Depósito Académico Digital Universidad de Navarra
dc.subject.none.fl_str_mv Serum
Sterols
COPs
Linearity
Recovery
Repeatability
topic Serum
Sterols
COPs
Linearity
Recovery
Repeatability
description A validated gas chromatography-mass spectrometry (GC-MS) detection method for the quantitative analysis of sterol oxidation products (SOPs) in serum is described. After a lipid extraction procedure with chloroform-methanol, a cold saponification and purification by solid phase extraction, oxysterols were derivatized to form trimethyl-sylil-ethers which were subjected to GC-MS analysis. Calibration curves for cholesterol oxidation products showed determination coefficient (R(2)) of 1.0, with low values for the coefficient of variation of the response factors (< 1%). Detection and quantification limits were below 5 ng/mL and 10 ng/mL, respectively. Recovery data were between 77.65% and 110.29% (CV < 10% for all compounds). Good results were obtained for within- and between-day repeatability, with values below 10%. In conclusion, the method performed is suitable for the determination and quantification of SOPs in serum.
publishDate 2008
dc.date.none.fl_str_mv 2008
2008-01-01
2008
2008-01-01
2012
2012-05-18
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10171/22124
url https://hdl.handle.net/10171/22124
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra
instname:Universidad de Navarra
instname_str Universidad de Navarra
reponame_str Dadun. Depósito Académico Digital de la Universidad de Navarra
collection Dadun. Depósito Académico Digital de la Universidad de Navarra
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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