Determination of free and derivative terpenoids and sterols in spent coffee grounds by two-dimensional liquid chromatography coupled to high-resolution mass spectrometry
Spent coffee grounds (SCGs) are a major global waste, yet rich in valuable bioactive compounds. Unlocking their potential requires advanced analytical techniques due to the complexity of their molecular composition. This study presents an innovative RPLC × SFC-HRMS method—an online two-dimensional l...
| Autores: | , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión aceptada para publicación |
| Fecha de publicación: | 2025 |
| País: | España |
| Institución: | Universidad de Jaén |
| Repositorio: | RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaén |
| OAI Identifier: | oai:ruja.ujaen.es:10953/6320 |
| Acceso en línea: | https://doi.org/10.1016/j.foodchem.2025.144853 https://hdl.handle.net/10953/6320 |
| Access Level: | acceso abierto |
| Palabra clave: | Spent coffee grounds liquid chromatography, supercritical fluid chromatography, online LCxSFC-HRMS sterols diterpenes 2301.03 2301.10 |
| Sumario: | Spent coffee grounds (SCGs) are a major global waste, yet rich in valuable bioactive compounds. Unlocking their potential requires advanced analytical techniques due to the complexity of their molecular composition. This study presents an innovative RPLC × SFC-HRMS method—an online two-dimensional liquid chromatography approach—for simultaneous analysis of sterol and diterpene derivatives in SCGs. Using a custom-built interface, both free and esterified lipid fractions were analyzed directly from intact, non-saponified and non-derivatized extracts. The method effectively resolved isomeric compounds and sterol oxidation products, highlighting its power for comprehensive lipid profiling and paving the way for SCG valorization in health-focused applications. |
|---|