Visual-area-specific tonic modulation of GABA release by endocannabinoids sets the activity and coordination of neocortical principal neurons

Perisomatic inhibition of pyramidal neurons (PNs) coordinates cortical network activity during sensory processing, and this role is mainly attributed to parvalbumin-expressing basket cells (BCs). However, cannabinoid receptor type 1 (CB1)-expressing interneurons are also BCs, but the connectivity an...

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Autores: Koukouli, F., Montmerle, M., Aguirre, A., De Brito Van Velze, M., Peixoto, J., Choudhary, V., Varilh, Marjorie, Julio-Kalajzic, Francisca, Allene, C., Méndez, Pablo, Zerlaut, Y., Marsicano, G., Schlüter, O.M., Rebola, Nelson, Bacci, Alberto, Lourenço, J.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/284949
Acceso en línea:http://hdl.handle.net/10261/284949
Access Level:acceso abierto
Palabra clave:CP: Neuroscience
endocannabinoids
Inhibition
interneurons
in vivo spontaneous activity
synaptic transmission
visual cortex.
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spelling Visual-area-specific tonic modulation of GABA release by endocannabinoids sets the activity and coordination of neocortical principal neuronsKoukouli, F.Montmerle, M.Aguirre, A.De Brito Van Velze, M.Peixoto, J.Choudhary, V.Varilh, MarjorieJulio-Kalajzic, FranciscaAllene, C.Méndez, PabloZerlaut, Y.Marsicano, G.Schlüter, O.M.Rebola, NelsonBacci, AlbertoLourenço, J.CP: NeuroscienceendocannabinoidsInhibitioninterneuronsin vivo spontaneous activitysynaptic transmissionvisual cortex.Perisomatic inhibition of pyramidal neurons (PNs) coordinates cortical network activity during sensory processing, and this role is mainly attributed to parvalbumin-expressing basket cells (BCs). However, cannabinoid receptor type 1 (CB1)-expressing interneurons are also BCs, but the connectivity and function of these elusive but prominent neocortical inhibitory neurons are unclear. We find that their connectivity pattern is visual area specific. Persistently active CB1 signaling suppresses GABA release from CB1 BCs in the medial secondary visual cortex (V2M), but not in the primary visual cortex (V1). Accordingly, in vivo, tonic CB1 signaling is responsible for higher but less coordinated PN activity in the V2M than in the V1. These differential firing dynamics in the V1 and V2M can be captured by a computational network model that incorporates visual-area-specific properties. Our results indicate a differential CB1-mediated mechanism controlling PN activity, suggesting an alternative connectivity scheme of a specific GABAergic circuit in different cortical areas.We thank Brahim Abbes for providing the Fiji plugin (Neurostack) for soma colocalization analysis and quantification and Ronan Chéreau and Michael Graupner for initial help with 2P imaging implementation. We also thank members of the Bacci laboratory for helpful discussions and the ICM technical staff of the facilities PHENO-ICMICE, iGENSEQ, and Histomics. All animal work was conducted at the PHENO-ICMice facility. We gratefully acknowledge Joanna Droesbeke for performing part of the transcardial perfusions. F.K. thanks the Fondation des Treilles for awarding her the 2020 Young Re-searchers Prize. This work was supported by ‘‘Investissements d’avenir’’ ANR-10-IAIHU-06, Agence Nationale de la Recherche (ANR-13-BSV4-0015-01, ANR-16-CE16-0007-02, ANR-17-CE16-0026-01, ANR-18-CE16-0011-01, and ANR-20-CE16-0011-01), Fondation pour la Recherche Médicale (Equipes FRM – DEQ20150331684, Equipes FRM – EQU201903007860), DIM Region Ile de France, and a grant from the Institut du Cerveau et de la Moelle épiniére (Paris) (to A.B.); European Research Council starting grant 678250) and the Brain & Behavior Research Foundation (NARSAD Young Investigator Grant) (to N.R.); Fondation pour la Recherche Médicale (ARF 201909009117) (to Y.Z.); the German Research Foundation through the Cluster of Excellence (EXC2067) Multiscale Imaging and the Collaborative Research Center 889 (project B3) (to O.M.S.). The Core ICM facilities were supported by 2 ‘‘Investissements d’avenir’’ (ANR-10- IAIHU-06 and ANR-11-INBS-0011-NeurATRIS) and the ‘‘Fondation pour la Recherche Médicale’’. This project has received funding from the European Union’s Horizon 2020 research and innovation program under Marie Skłodowska-Curie grant agreement 892175 (to Y.Z.).Cell PressAgence Nationale de la Recherche (France)Fondation pour la Recherche MédicaleInstitut du Cerveau et de la Moelle Epinière (France)European Research CouncilEuropean CommissionConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2022202220222022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/284949reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.celrep.2022.111202Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2849492026-05-22T06:33:51Z
dc.title.none.fl_str_mv Visual-area-specific tonic modulation of GABA release by endocannabinoids sets the activity and coordination of neocortical principal neurons
title Visual-area-specific tonic modulation of GABA release by endocannabinoids sets the activity and coordination of neocortical principal neurons
spellingShingle Visual-area-specific tonic modulation of GABA release by endocannabinoids sets the activity and coordination of neocortical principal neurons
Koukouli, F.
CP: Neuroscience
endocannabinoids
Inhibition
interneurons
in vivo spontaneous activity
synaptic transmission
visual cortex.
title_short Visual-area-specific tonic modulation of GABA release by endocannabinoids sets the activity and coordination of neocortical principal neurons
title_full Visual-area-specific tonic modulation of GABA release by endocannabinoids sets the activity and coordination of neocortical principal neurons
title_fullStr Visual-area-specific tonic modulation of GABA release by endocannabinoids sets the activity and coordination of neocortical principal neurons
title_full_unstemmed Visual-area-specific tonic modulation of GABA release by endocannabinoids sets the activity and coordination of neocortical principal neurons
title_sort Visual-area-specific tonic modulation of GABA release by endocannabinoids sets the activity and coordination of neocortical principal neurons
dc.creator.none.fl_str_mv Koukouli, F.
Montmerle, M.
Aguirre, A.
De Brito Van Velze, M.
Peixoto, J.
Choudhary, V.
Varilh, Marjorie
Julio-Kalajzic, Francisca
Allene, C.
Méndez, Pablo
Zerlaut, Y.
Marsicano, G.
Schlüter, O.M.
Rebola, Nelson
Bacci, Alberto
Lourenço, J.
author Koukouli, F.
author_facet Koukouli, F.
Montmerle, M.
Aguirre, A.
De Brito Van Velze, M.
Peixoto, J.
Choudhary, V.
Varilh, Marjorie
Julio-Kalajzic, Francisca
Allene, C.
Méndez, Pablo
Zerlaut, Y.
Marsicano, G.
Schlüter, O.M.
Rebola, Nelson
Bacci, Alberto
Lourenço, J.
author_role author
author2 Montmerle, M.
Aguirre, A.
De Brito Van Velze, M.
Peixoto, J.
Choudhary, V.
Varilh, Marjorie
Julio-Kalajzic, Francisca
Allene, C.
Méndez, Pablo
Zerlaut, Y.
Marsicano, G.
Schlüter, O.M.
Rebola, Nelson
Bacci, Alberto
Lourenço, J.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Agence Nationale de la Recherche (France)
Fondation pour la Recherche Médicale
Institut du Cerveau et de la Moelle Epinière (France)
European Research Council
European Commission
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv CP: Neuroscience
endocannabinoids
Inhibition
interneurons
in vivo spontaneous activity
synaptic transmission
visual cortex.
topic CP: Neuroscience
endocannabinoids
Inhibition
interneurons
in vivo spontaneous activity
synaptic transmission
visual cortex.
description Perisomatic inhibition of pyramidal neurons (PNs) coordinates cortical network activity during sensory processing, and this role is mainly attributed to parvalbumin-expressing basket cells (BCs). However, cannabinoid receptor type 1 (CB1)-expressing interneurons are also BCs, but the connectivity and function of these elusive but prominent neocortical inhibitory neurons are unclear. We find that their connectivity pattern is visual area specific. Persistently active CB1 signaling suppresses GABA release from CB1 BCs in the medial secondary visual cortex (V2M), but not in the primary visual cortex (V1). Accordingly, in vivo, tonic CB1 signaling is responsible for higher but less coordinated PN activity in the V2M than in the V1. These differential firing dynamics in the V1 and V2M can be captured by a computational network model that incorporates visual-area-specific properties. Our results indicate a differential CB1-mediated mechanism controlling PN activity, suggesting an alternative connectivity scheme of a specific GABAergic circuit in different cortical areas.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/284949
url http://hdl.handle.net/10261/284949
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.celrep.2022.111202

dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Cell Press
publisher.none.fl_str_mv Cell Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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