Activity-dependent switch of gabaergic inhibition into glutamatergic excitation in astrocyte-neuron networks

Interneurons are critical for proper neural network function and can activate Ca2 signaling in astrocytes. However, the impact of the interneuron-astrocyte signaling into neuronal network operation remains unknown. Using the simplest hippocampal Astrocyte-Neuron network, i.e., GABAergic interneuron,...

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Detalles Bibliográficos
Autores: Perea, Gertrudis, Gómez, R., Mederos, Sara, Covelo, Ana, Ballesteros, J.J., Schlosser, L., Hernández-Vivanco, Alicia, Martín-Fernández, Mario, Quintana-Cabrera, Ruben, Rayan, A., Díez, A., Fuenzalida, M., Agarwal, A., Bergles, D. E., Bettler, B., Manahan-Vaughan, D., Martín, E.D., Kirchhoff, F., Araque, Alfonso
Tipo de recurso: artículo
Fecha de publicación:2016
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/158939
Acceso en línea:http://hdl.handle.net/10261/158939
Access Level:acceso abierto
Palabra clave:neuron-glia interactions
interneuron
Astrocytes
Neuroscience
rat
synaptic plasticity
Mouse
Descripción
Sumario:Interneurons are critical for proper neural network function and can activate Ca2 signaling in astrocytes. However, the impact of the interneuron-astrocyte signaling into neuronal network operation remains unknown. Using the simplest hippocampal Astrocyte-Neuron network, i.e., GABAergic interneuron, pyramidal neuron, single CA3-CA1 glutamatergic synapse, and astrocytes, we found that interneuron-astrocyte signaling dynamically affected excitatory neurotransmission in an activity- and time-dependent manner, and determined the sign (inhibition vs potentiation) of the GABA-mediated effects. While synaptic inhibition was mediated by GABAA receptors, potentiation involved astrocyte GABAB receptors, astrocytic glutamate release, and presynaptic metabotropic glutamate receptors. Using conditional astrocyte-specific GABAB receptor (Gabbr1) knockout mice, we confirmed the glial source of the interneuron-induced potentiation, and demonstrated the involvement of astrocytes in hippocampal theta and gamma oscillations in vivo. Therefore, astrocytes decode interneuron activity and transform inhibitory into excitatory signals, contributing to the emergence of novel network properties resulting from the interneuron-astrocyte interplay.

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