DIPA-CRISPR is a simple and accessible method for insect gene editing

Current approaches for insect gene editing require microinjection of materials into early embryos. This severely limits the application of gene editing to a great number of insect species, especially to those whose reproduction systems preclude access to early embryos for injection. To overcome thes...

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Detalles Bibliográficos
Autores: Shirai, Yu, Piulachs, Maria-Dolors, Bellés, Xavier, Daimon, Takaaki
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/272293
Acceso en línea:http://hdl.handle.net/10261/272293
Access Level:acceso abierto
Palabra clave:CRISPR-Cas9
DIPA-CRISPR
Gene editing
Genome editing
Cockroaches
Beetles
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spelling DIPA-CRISPR is a simple and accessible method for insect gene editingShirai, YuPiulachs, Maria-DolorsBellés, XavierDaimon, TakaakiCRISPR-Cas9DIPA-CRISPRGene editingGenome editingCockroachesBeetlesCurrent approaches for insect gene editing require microinjection of materials into early embryos. This severely limits the application of gene editing to a great number of insect species, especially to those whose reproduction systems preclude access to early embryos for injection. To overcome these limitations, we report a simple and accessible method for insect gene editing, termed “direct parental” CRISPR (DIPA-CRISPR). We show that injection of Cas9 ribonucleoproteins (RNPs) into the haemocoel of adult females efficiently introduces heritable mutations in developing oocytes. Importantly, commercially available standard Cas9 protein can be directly used for DIPA-CRISPR, which makes this approach highly practical and feasible. DIPA-CRISPR enables highly efficient gene editing in the cockroaches, on which conventional approaches cannot be applied, and in the model beetle Tribolium castaneum. Due to its simplicity and accessibility, DIPA-CRISPR will greatly extend the application of gene editing technology to a wide variety of insects.This research was supported by JSPS KAKENHI (nos. 20H02999 and 20K21311 to T.D.); JSPS Open Partnership Joint Research Projects (no. JPJSBP120209917 to T.D.); Spanish Ministry of Innovation and Competitiveness (PID2019-104483GB-I00 to X.B.); CSIC-Spain (2019AEP028 to M.-D.P.); and in part by Cabinet Office, Government of Japan, Cross-ministerial Moonshot Agriculture, Forestry and Fisheries Research and Development Program (no. JPJ009237 to T.D.). Y.S. is a JSPS Research Fellow with a research grant (DC1, 21J20658).Peer reviewedElsevierJapan Society for the Promotion of ScienceMinisterio de Ciencia e Innovación (España)Consejo Superior de Investigaciones Científicas (España)Ministry of Agriculture, Forestry and Fisheries (Japan)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/272293reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-104483GB-I00The underlying dataset has been published as supplementary material of the article in the publisher platform at 10.1016/j.crmeth.2022.100215https://doi.org/10.1016/j.crmeth.2022.100215Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2722932026-05-22T06:33:51Z
dc.title.none.fl_str_mv DIPA-CRISPR is a simple and accessible method for insect gene editing
title DIPA-CRISPR is a simple and accessible method for insect gene editing
spellingShingle DIPA-CRISPR is a simple and accessible method for insect gene editing
Shirai, Yu
CRISPR-Cas9
DIPA-CRISPR
Gene editing
Genome editing
Cockroaches
Beetles
title_short DIPA-CRISPR is a simple and accessible method for insect gene editing
title_full DIPA-CRISPR is a simple and accessible method for insect gene editing
title_fullStr DIPA-CRISPR is a simple and accessible method for insect gene editing
title_full_unstemmed DIPA-CRISPR is a simple and accessible method for insect gene editing
title_sort DIPA-CRISPR is a simple and accessible method for insect gene editing
dc.creator.none.fl_str_mv Shirai, Yu
Piulachs, Maria-Dolors
Bellés, Xavier
Daimon, Takaaki
author Shirai, Yu
author_facet Shirai, Yu
Piulachs, Maria-Dolors
Bellés, Xavier
Daimon, Takaaki
author_role author
author2 Piulachs, Maria-Dolors
Bellés, Xavier
Daimon, Takaaki
author2_role author
author
author
dc.contributor.none.fl_str_mv Japan Society for the Promotion of Science
Ministerio de Ciencia e Innovación (España)
Consejo Superior de Investigaciones Científicas (España)
Ministry of Agriculture, Forestry and Fisheries (Japan)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv CRISPR-Cas9
DIPA-CRISPR
Gene editing
Genome editing
Cockroaches
Beetles
topic CRISPR-Cas9
DIPA-CRISPR
Gene editing
Genome editing
Cockroaches
Beetles
description Current approaches for insect gene editing require microinjection of materials into early embryos. This severely limits the application of gene editing to a great number of insect species, especially to those whose reproduction systems preclude access to early embryos for injection. To overcome these limitations, we report a simple and accessible method for insect gene editing, termed “direct parental” CRISPR (DIPA-CRISPR). We show that injection of Cas9 ribonucleoproteins (RNPs) into the haemocoel of adult females efficiently introduces heritable mutations in developing oocytes. Importantly, commercially available standard Cas9 protein can be directly used for DIPA-CRISPR, which makes this approach highly practical and feasible. DIPA-CRISPR enables highly efficient gene editing in the cockroaches, on which conventional approaches cannot be applied, and in the model beetle Tribolium castaneum. Due to its simplicity and accessibility, DIPA-CRISPR will greatly extend the application of gene editing technology to a wide variety of insects.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/272293
url http://hdl.handle.net/10261/272293
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-104483GB-I00
The underlying dataset has been published as supplementary material of the article in the publisher platform at 10.1016/j.crmeth.2022.100215
https://doi.org/10.1016/j.crmeth.2022.100215
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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repository.mail.fl_str_mv
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