Extracellular vesicles-based biomarkers represent a promising liquid biopsy in endometrial cancer

© 2019 by the authors.

Detalles Bibliográficos
Autores: Herrero, Carolina, Fuente, Alexandre de la, Casas-Arozamena, Carlos, Sebastián, Víctor, Prieto, Martín, Arruebo, Manuel, Abalo, Alicia, Colas, Eva, Moreno-Bueno, Gema, Gil-Moreno, Antonio, Vilar, Ana, Cueva, Juan, Abal, Miguel, Muinelo-Romay, Laura
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/206674
Acceso en línea:http://hdl.handle.net/10261/206674
Access Level:acceso abierto
Palabra clave:EVs
ExoGAG
Endometrial cancer
ANXA2
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spelling Extracellular vesicles-based biomarkers represent a promising liquid biopsy in endometrial cancerHerrero, CarolinaFuente, Alexandre de laCasas-Arozamena, CarlosSebastián, VíctorPrieto, MartínArruebo, ManuelAbalo, AliciaColas, EvaMoreno-Bueno, GemaGil-Moreno, AntonioVilar, AnaCueva, JuanAbal, MiguelMuinelo-Romay, LauraEVsExoGAGEndometrial cancerANXA2© 2019 by the authors.Tumor-derived extracellular vesicles (EVs) are secreted in large amounts into biological fluids of cancer patients. The analysis of EVs cargoes has been associated with patient´s outcome and response to therapy. However, current technologies for EVs isolation are tedious and low cost-efficient for routine clinical implementation. To explore the clinical value of circulating EVs analysis we attempted a proof-of-concept in endometrial cancer (EC) with ExoGAG, an easy to use and highly efficient new technology to enrich EVs. Technical performance was first evaluated using EVs secreted by Hec1A cells. Then, the clinical value of this strategy was questioned by analyzing the levels of two well-known tissue biomarkers in EC, L1 cell adhesion molecule (L1CAM) and Annexin A2 (ANXA2), in EVs purified from plasma in a cohort of 41 EC patients and 20 healthy controls. The results demonstrated the specific content of ANXA2 in the purified EVs fraction, with an accurate sensitivity and specificity for EC diagnosis. Importantly, high ANXA2 levels in circulating EVs were associated with high risk of recurrence and non-endometrioid histology suggesting a potential value as a prognostic biomarker in EC. These results also confirmed ExoGAG technology as a robust technique for the clinical implementation of circulating EVs analyses.This research was funded by Instituto de Salud Carlos III, grant PI17/01919, co-financed by the European Regional Development Fund (FEDER), and by Fundación Científica de la Asociación Española Contra el Cáncer (AECC), Grupos Clínicos Coordinados 2018. Carolina Herrero is supported by a predoctoral i-PFIS fellowship from Instituto de Salud Carlos III (IFI17/00047); Laura Muinelo is supported by Asociación Española Contra el Cáncer (AECC).Multidisciplinary Digital Publishing InstituteInstituto de Salud Carlos IIIEuropean CommissionAsociación Española Contra el CáncerConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2020202020192020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/206674reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.3390/cancers11122000Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2066742026-05-22T06:33:51Z
dc.title.none.fl_str_mv Extracellular vesicles-based biomarkers represent a promising liquid biopsy in endometrial cancer
title Extracellular vesicles-based biomarkers represent a promising liquid biopsy in endometrial cancer
spellingShingle Extracellular vesicles-based biomarkers represent a promising liquid biopsy in endometrial cancer
Herrero, Carolina
EVs
ExoGAG
Endometrial cancer
ANXA2
title_short Extracellular vesicles-based biomarkers represent a promising liquid biopsy in endometrial cancer
title_full Extracellular vesicles-based biomarkers represent a promising liquid biopsy in endometrial cancer
title_fullStr Extracellular vesicles-based biomarkers represent a promising liquid biopsy in endometrial cancer
title_full_unstemmed Extracellular vesicles-based biomarkers represent a promising liquid biopsy in endometrial cancer
title_sort Extracellular vesicles-based biomarkers represent a promising liquid biopsy in endometrial cancer
dc.creator.none.fl_str_mv Herrero, Carolina
Fuente, Alexandre de la
Casas-Arozamena, Carlos
Sebastián, Víctor
Prieto, Martín
Arruebo, Manuel
Abalo, Alicia
Colas, Eva
Moreno-Bueno, Gema
Gil-Moreno, Antonio
Vilar, Ana
Cueva, Juan
Abal, Miguel
Muinelo-Romay, Laura
author Herrero, Carolina
author_facet Herrero, Carolina
Fuente, Alexandre de la
Casas-Arozamena, Carlos
Sebastián, Víctor
Prieto, Martín
Arruebo, Manuel
Abalo, Alicia
Colas, Eva
Moreno-Bueno, Gema
Gil-Moreno, Antonio
Vilar, Ana
Cueva, Juan
Abal, Miguel
Muinelo-Romay, Laura
author_role author
author2 Fuente, Alexandre de la
Casas-Arozamena, Carlos
Sebastián, Víctor
Prieto, Martín
Arruebo, Manuel
Abalo, Alicia
Colas, Eva
Moreno-Bueno, Gema
Gil-Moreno, Antonio
Vilar, Ana
Cueva, Juan
Abal, Miguel
Muinelo-Romay, Laura
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Salud Carlos III
European Commission
Asociación Española Contra el Cáncer
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv EVs
ExoGAG
Endometrial cancer
ANXA2
topic EVs
ExoGAG
Endometrial cancer
ANXA2
description © 2019 by the authors.
publishDate 2019
dc.date.none.fl_str_mv 2019
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/206674
url http://hdl.handle.net/10261/206674
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.3390/cancers11122000
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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