Extracellular vesicles-based biomarkers represent a promising liquid biopsy in endometrial cancer

Tumor-derived extracellular vesicles (EVs) are secreted in large amounts into biological fluids of cancer patients. The analysis of EVs cargoes has been associated with patient's outcome and response to therapy. However, current technologies for EVs isolation are tedious and low cost-efficient...

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Detalles Bibliográficos
Autores: Herrero, Cristina, De La Fuente, Adolfo, Casas-Arozamena, Carlos|||0000-0002-3279-2482, Sebastian, Víctor|||0000-0002-6873-5244, Prieto, Martín, Arruebo, Manuel, Abalo, Alicia, Colás Ortega, Eva|||0000-0003-0302-4828, Moreno-Bueno, Gema|||0000-0002-5030-6687, Gil-Moreno, Antonio|||0000-0003-1106-5590, Vilar, Ana, Cueva, Juan|||0000-0003-1389-4595, Abal Posada, Miguel|||0000-0003-3533-7781, Muinelo-Romay, Laura|||0000-0002-7456-7531
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:223895
Acceso en línea:https://ddd.uab.cat/record/223895
https://dx.doi.org/urn:doi:10.3390/cancers11122000
Access Level:acceso abierto
Palabra clave:EVs
ExoGAG
Endometrial cancer
ANXA2
Descripción
Sumario:Tumor-derived extracellular vesicles (EVs) are secreted in large amounts into biological fluids of cancer patients. The analysis of EVs cargoes has been associated with patient's outcome and response to therapy. However, current technologies for EVs isolation are tedious and low cost-efficient for routine clinical implementation. To explore the clinical value of circulating EVs analysis we attempted a proof-of-concept in endometrial cancer (EC) with ExoGAG, an easy to use and highly efficient new technology to enrich EVs. Technical performance was first evaluated using EVs secreted by Hec1A cells. Then, the clinical value of this strategy was questioned by analyzing the levels of two well-known tissue biomarkers in EC, L1 cell adhesion molecule (L1CAM) and Annexin A2 (ANXA2), in EVs purified from plasma in a cohort of 41 EC patients and 20 healthy controls. The results demonstrated the specific content of ANXA2 in the purified EVs fraction, with an accurate sensitivity and specificity for EC diagnosis. Importantly, high ANXA2 levels in circulating EVs were associated with high risk of recurrence and non-endometrioid histology suggesting a potential value as a prognostic biomarker in EC. These results also confirmed ExoGAG technology as a robust technique for the clinical implementation of circulating EVs analyses.

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