Methodological Assessment of ExoGAG for Isolation of Cerebrospinal Fluid Extracellular Vesicles as a Source of Biomarkers

Extracellular vesicles (EVs) in cerebrospinal fluid (CSF) represent a valuable source of biomarkers for central nervous system (CNS) diseases, offering new pathways for diagnosis and monitoring. However, existing methods for isolating EVs from CSF often prove to be labor-intensive and reliant on spe...

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Detalhes bibliográficos
Autores: Salvat-Rovira, Nil|||0009-0005-8515-3819, Vázquez-Oliver, Anna|||0000-0002-9842-7656, Rivas Asensio, Elisa, Herrero-Lorenzo, Marina|||0000-0003-2693-0700, Gámez-Valero, Ana|||0000-0002-9530-2751, Pérez-Pérez, Jesús|||0000-0002-9835-0484, Izquierdo, Cristina, Campolongo, Antonia|||0000-0002-7962-3264, Martí, Eulàlia|||0000-0002-1030-3158, Kulisevsky, Jaime|||0000-0003-4870-1431, Pérez-González, Rocío|||0000-0002-0641-2001
Formato: artículo
Fecha de publicación:2024
País:España
Recursos:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:305975
Acesso em linha:https://ddd.uab.cat/record/305975
https://dx.doi.org/urn:doi:10.3390/ijms252413705
Access Level:acceso abierto
Palavra-chave:Extracellular vesicles
Cerebrospinal fluid
Isolation method
Ultracentrifugation
ExoGAG
Biomarkers
Mirnas
Descrição
Resumo:Extracellular vesicles (EVs) in cerebrospinal fluid (CSF) represent a valuable source of biomarkers for central nervous system (CNS) diseases, offering new pathways for diagnosis and monitoring. However, existing methods for isolating EVs from CSF often prove to be labor-intensive and reliant on specialized equipment, hindering their clinical application. In this study, we present a novel, clinically compatible method for isolating EVs from CSF. We optimized the use of ExoGAG, a commercially available reagent that has been tested in plasma, urine and semen, and compared it directly with differential ultracentrifugation using Western blotting, protein quantification, nanoparticle tracking analysis, and cryogenic electron microscopy. Additionally, we analyzed the presence of specific microRNAs (miRNAs) known to be present in CSF-derived EVs. Our data demonstrate that ExoGAG is an effective method for isolating EVs from CSF, yielding a higher amount of EVs compared to traditional ultracentrifugation methods, and with comparable levels of specific miRNAs. In conclusion, the use of ExoGAG in a clinical setting may facilitate the testing of biomarkers essential for tracking brain pathology in CNS diseases.

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