Synergistic inhibition of pancreatic lipase by olive phenolics isolated from olive oil by-products: hydroxytyrosol, 3,4-dihydroxyphenylglycol, and comselogoside

11 Páginas.-- 5 Figuras.-- 2 Tablas

Detalles Bibliográficos
Autores: Rubio-Senent, Fátima, Bermúdez-Oria, Alejandra, Fernández-Prior, África, Lama Muñoz, Antonio, Rodríguez-Gutiérrez, Guillermo, Fernández-Bolaños Guzmán, Juan
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2026
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/417286
Acceso en línea:http://hdl.handle.net/10261/417286
Access Level:acceso abierto
Palabra clave:Pancreatic lipase inhibition
Olive by-products
Synergistic effect
Isobologram
Combination index
Dose-reduction index
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spelling Synergistic inhibition of pancreatic lipase by olive phenolics isolated from olive oil by-products: hydroxytyrosol, 3,4-dihydroxyphenylglycol, and comselogosideRubio-Senent, FátimaBermúdez-Oria, AlejandraFernández-Prior, ÁfricaLama Muñoz, AntonioRodríguez-Gutiérrez, GuillermoFernández-Bolaños Guzmán, JuanPancreatic lipase inhibitionOlive by-productsSynergistic effectIsobologramCombination indexDose-reduction index11 Páginas.-- 5 Figuras.-- 2 TablasObesity remains a major global health challenge, and pancreatic lipase (PL) inhibition represents a key strategy to reduce dietary fat absorption. This study evaluated the individual and combined inhibitory effects of three important olive phenolic compounds—hydroxytyrosol (HT), 3,4-dihydroxyphenylglycol (DHPG), and comselogoside (COM)—on PL activity in vitro. All three compounds showed concentration-dependent inhibition, with HT being the most potent (IC₅₀ = 0.294 mM), followed by DHPG (IC₅₀ = 0.464 mM) and COM (IC₅₀ = 0.858 mM). Kinetic analyses revealed competitive inhibition for HT and non-competitive inhibition for DHPG. Binary mixtures of HT:DHPG and HT:COM were tested at seven molar ratios (10:1, 5:1, 2:1, 1:1, 1:2, 1:5, and 1:10). Both combinations showed clear dose-dependent inhibition and marked synergistic effects across most molar ratios and inhibition levels (50–97%), as demonstrated by combination index (CI < 1) and isobologram analysis. The strongest synergism occurred at the 1:1 ratio, with CI values between 0.09 and 0.30 and high dose-reduction index (DRI) values (5–83-fold), indicating enhanced inhibitory potency at lower concentrations. Kinetic analyses confirmed that the HT:DHPG mixture followed a non-competitive pattern, whereas HT:COM maintained a competitive mechanism. These findings demonstrate that olive phenolics can act cooperatively to enhance PL inhibition, highlighting their potential as natural and sustainable agents for the development of functional foods and nutraceuticals aimed at lipid digestion and obesity management.This work was supported by the Spanish Ministry of Science, Innovation and Universities (grant number PID2022-142731OB-C21).Peer reviewedElsevierMinisterio de Ciencia, Innovación y Universidades (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202620262026info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/417286reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-142731OB-C21https://doi.org/10.1016/j.fufo.2026.100937Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/4172862026-05-22T06:33:51Z
dc.title.none.fl_str_mv Synergistic inhibition of pancreatic lipase by olive phenolics isolated from olive oil by-products: hydroxytyrosol, 3,4-dihydroxyphenylglycol, and comselogoside
title Synergistic inhibition of pancreatic lipase by olive phenolics isolated from olive oil by-products: hydroxytyrosol, 3,4-dihydroxyphenylglycol, and comselogoside
spellingShingle Synergistic inhibition of pancreatic lipase by olive phenolics isolated from olive oil by-products: hydroxytyrosol, 3,4-dihydroxyphenylglycol, and comselogoside
Rubio-Senent, Fátima
Pancreatic lipase inhibition
Olive by-products
Synergistic effect
Isobologram
Combination index
Dose-reduction index
title_short Synergistic inhibition of pancreatic lipase by olive phenolics isolated from olive oil by-products: hydroxytyrosol, 3,4-dihydroxyphenylglycol, and comselogoside
title_full Synergistic inhibition of pancreatic lipase by olive phenolics isolated from olive oil by-products: hydroxytyrosol, 3,4-dihydroxyphenylglycol, and comselogoside
title_fullStr Synergistic inhibition of pancreatic lipase by olive phenolics isolated from olive oil by-products: hydroxytyrosol, 3,4-dihydroxyphenylglycol, and comselogoside
title_full_unstemmed Synergistic inhibition of pancreatic lipase by olive phenolics isolated from olive oil by-products: hydroxytyrosol, 3,4-dihydroxyphenylglycol, and comselogoside
title_sort Synergistic inhibition of pancreatic lipase by olive phenolics isolated from olive oil by-products: hydroxytyrosol, 3,4-dihydroxyphenylglycol, and comselogoside
dc.creator.none.fl_str_mv Rubio-Senent, Fátima
Bermúdez-Oria, Alejandra
Fernández-Prior, África
Lama Muñoz, Antonio
Rodríguez-Gutiérrez, Guillermo
Fernández-Bolaños Guzmán, Juan
author Rubio-Senent, Fátima
author_facet Rubio-Senent, Fátima
Bermúdez-Oria, Alejandra
Fernández-Prior, África
Lama Muñoz, Antonio
Rodríguez-Gutiérrez, Guillermo
Fernández-Bolaños Guzmán, Juan
author_role author
author2 Bermúdez-Oria, Alejandra
Fernández-Prior, África
Lama Muñoz, Antonio
Rodríguez-Gutiérrez, Guillermo
Fernández-Bolaños Guzmán, Juan
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Pancreatic lipase inhibition
Olive by-products
Synergistic effect
Isobologram
Combination index
Dose-reduction index
topic Pancreatic lipase inhibition
Olive by-products
Synergistic effect
Isobologram
Combination index
Dose-reduction index
description 11 Páginas.-- 5 Figuras.-- 2 Tablas
publishDate 2026
dc.date.none.fl_str_mv 2026
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/417286
url http://hdl.handle.net/10261/417286
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-142731OB-C21
https://doi.org/10.1016/j.fufo.2026.100937
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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