Freshwater-adapted polychaetes exhibit a complete enzymatic machinery for synthesizing long-chain polyunsaturated fatty acids

[EN] The sustainability of aquaculture is challenged by limited fishmeal and fish oil supplies, key sources of long-chain polyunsaturated fatty acids (LC-PUFA) such as eicosapentaenoic acid (EPA, 20:5 n-3), docosahexaenoic acid (DHA, 22:6 n-3) and arachidonic acid (ARA, 20:4 n-6), essential for fish...

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Autores: Bainour, Khalida, Zulkifli, Nabilah, Sam, Ka-Kei, Navarro, Juan C., Castro, Luis Filipe C., Glasby, Christopher J., Shu-Chien, Alexander C., Monroig, Óscar
Tipo de recurso: artículo
Fecha de publicación:2025
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:dnet:riunet______::df94829716861747e8303a28b9b70b5d
Acceso en línea:https://riunet.upv.es/handle/10251/235489
Access Level:acceso abierto
Palabra clave:Elovl
Fatty acyl desaturases
Freshwater polychaetes
LC-PUFA biosynthesis
Namalycastis rhodochorde
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spelling Freshwater-adapted polychaetes exhibit a complete enzymatic machinery for synthesizing long-chain polyunsaturated fatty acidsBainour, KhalidaZulkifli, NabilahSam, Ka-KeiNavarro, Juan C.Castro, Luis Filipe C.Glasby, Christopher J.Shu-Chien, Alexander C.Monroig, ÓscarElovlFatty acyl desaturasesFreshwater polychaetesLC-PUFA biosynthesisNamalycastis rhodochorde[EN] The sustainability of aquaculture is challenged by limited fishmeal and fish oil supplies, key sources of long-chain polyunsaturated fatty acids (LC-PUFA) such as eicosapentaenoic acid (EPA, 20:5 n-3), docosahexaenoic acid (DHA, 22:6 n-3) and arachidonic acid (ARA, 20:4 n-6), essential for fish health and product quality. Polychaetes represent a promising alternative. While marine polychaetes show complete LC-PUFA biosynthetic pathways involving elongases (Elovl), front-end desaturases (Fed), and methyl-end desaturases (¿ des), freshwater species remain poorly studied. We hypothesize that freshwater-adapted polychaetes exhibit enhanced LC-PUFA biosynthesis to compensate for limited dietary sources in freshwater environments. This study focuses on Namalycastis rhodochorde, a freshwater nereid polychaete found in Southeast Asia. We isolated and characterized elongase and desaturase genes from N. rhodochorde using a yeast-based heterologous expression system. Our results revealed three Elovl (Elovl2/5, Elovl4, Elovl1/7) that elongate PUFA substrates from C18 to C22, two Fed (Fed1 with ¿5 and Fed2 with dual ¿6/¿8 activities), and two ¿ des: a ¿12 desaturase enabling linoleic acid (18:2 n-6) synthesis, and an ¿3 desaturase converting n-6 into n-3 PUFA. These findings indicate that N. rhodochorde has the enzymatic capacity to synthesize LC-PUFA like ARA and EPA, supporting its potential for sustainable biomass production using low-nutrient substrates.The POLYPUFA grant PID2022-136234OB-C21 was funded by MICIU/AEI/10.13039/501100011033 and by FEDER, UE. Additionally, this study is part of the ThinkInAzul programme funded by the European Union NextGenerationEU (PRTR-C17. I1) and the Generalitat Valenciana (THINKINAZUL/2021/026). K.B. is funded by a predoctoral contract, Santiago Grisolia (GRISOLIA/2021/120), from the Generalitat Valenciana. K.-K.S. acknowledges funding from a postdoctoral contract with Universiti Sains Malaysia (USM.9/25 Jld.XIV). We are also grateful to the Malaysian Ministry of Higher Education for research funding to A.C.S.-C. and graduate assistantship to N.Z. through the Fundamental Research Grant Scheme FRGS/1/2022/STG03/USM/01/2.The Royal Society PublishingGeneralitat ValencianaUniversity of Science, MalaysiaAgencia Estatal de InvestigaciónEuropean Regional Development FundMinistry of Higher Education, MalaysiaRepositorio Institucional de la Universitat Politècnica de València Riunet20252025-09-03journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/235489reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023 PID2022-136234OB-C21 ESTUDIO DE LOS MECANISMOS DE BIOSINTESIS DE OMEGA-3 EN POLIQUETOS PARA SU USO COMO RECURSO EN ACUICULTURAGeneralitat Valenciana https://doi.org/10.13039/501100003359 GRISOLIA%2F2021%2F120Generalitat Valenciana https://doi.org/10.13039/501100003359 THINKINAZUL%2F2021%2F026USM USM FRGS%2F1%2F2022%2FSTG03%2FUSM%2F01%2F2open accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento (by)http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:dnet:riunet______::df94829716861747e8303a28b9b70b5d2026-06-13T07:49:27Z
dc.title.none.fl_str_mv Freshwater-adapted polychaetes exhibit a complete enzymatic machinery for synthesizing long-chain polyunsaturated fatty acids
title Freshwater-adapted polychaetes exhibit a complete enzymatic machinery for synthesizing long-chain polyunsaturated fatty acids
spellingShingle Freshwater-adapted polychaetes exhibit a complete enzymatic machinery for synthesizing long-chain polyunsaturated fatty acids
Bainour, Khalida
Elovl
Fatty acyl desaturases
Freshwater polychaetes
LC-PUFA biosynthesis
Namalycastis rhodochorde
title_short Freshwater-adapted polychaetes exhibit a complete enzymatic machinery for synthesizing long-chain polyunsaturated fatty acids
title_full Freshwater-adapted polychaetes exhibit a complete enzymatic machinery for synthesizing long-chain polyunsaturated fatty acids
title_fullStr Freshwater-adapted polychaetes exhibit a complete enzymatic machinery for synthesizing long-chain polyunsaturated fatty acids
title_full_unstemmed Freshwater-adapted polychaetes exhibit a complete enzymatic machinery for synthesizing long-chain polyunsaturated fatty acids
title_sort Freshwater-adapted polychaetes exhibit a complete enzymatic machinery for synthesizing long-chain polyunsaturated fatty acids
dc.creator.none.fl_str_mv Bainour, Khalida
Zulkifli, Nabilah
Sam, Ka-Kei
Navarro, Juan C.
Castro, Luis Filipe C.
Glasby, Christopher J.
Shu-Chien, Alexander C.
Monroig, Óscar
author Bainour, Khalida
author_facet Bainour, Khalida
Zulkifli, Nabilah
Sam, Ka-Kei
Navarro, Juan C.
Castro, Luis Filipe C.
Glasby, Christopher J.
Shu-Chien, Alexander C.
Monroig, Óscar
author_role author
author2 Zulkifli, Nabilah
Sam, Ka-Kei
Navarro, Juan C.
Castro, Luis Filipe C.
Glasby, Christopher J.
Shu-Chien, Alexander C.
Monroig, Óscar
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Generalitat Valenciana
University of Science, Malaysia
Agencia Estatal de Investigación
European Regional Development Fund
Ministry of Higher Education, Malaysia
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Elovl
Fatty acyl desaturases
Freshwater polychaetes
LC-PUFA biosynthesis
Namalycastis rhodochorde
topic Elovl
Fatty acyl desaturases
Freshwater polychaetes
LC-PUFA biosynthesis
Namalycastis rhodochorde
description [EN] The sustainability of aquaculture is challenged by limited fishmeal and fish oil supplies, key sources of long-chain polyunsaturated fatty acids (LC-PUFA) such as eicosapentaenoic acid (EPA, 20:5 n-3), docosahexaenoic acid (DHA, 22:6 n-3) and arachidonic acid (ARA, 20:4 n-6), essential for fish health and product quality. Polychaetes represent a promising alternative. While marine polychaetes show complete LC-PUFA biosynthetic pathways involving elongases (Elovl), front-end desaturases (Fed), and methyl-end desaturases (¿ des), freshwater species remain poorly studied. We hypothesize that freshwater-adapted polychaetes exhibit enhanced LC-PUFA biosynthesis to compensate for limited dietary sources in freshwater environments. This study focuses on Namalycastis rhodochorde, a freshwater nereid polychaete found in Southeast Asia. We isolated and characterized elongase and desaturase genes from N. rhodochorde using a yeast-based heterologous expression system. Our results revealed three Elovl (Elovl2/5, Elovl4, Elovl1/7) that elongate PUFA substrates from C18 to C22, two Fed (Fed1 with ¿5 and Fed2 with dual ¿6/¿8 activities), and two ¿ des: a ¿12 desaturase enabling linoleic acid (18:2 n-6) synthesis, and an ¿3 desaturase converting n-6 into n-3 PUFA. These findings indicate that N. rhodochorde has the enzymatic capacity to synthesize LC-PUFA like ARA and EPA, supporting its potential for sustainable biomass production using low-nutrient substrates.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025-09-03
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/235489
url https://riunet.upv.es/handle/10251/235489
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023 PID2022-136234OB-C21 ESTUDIO DE LOS MECANISMOS DE BIOSINTESIS DE OMEGA-3 EN POLIQUETOS PARA SU USO COMO RECURSO EN ACUICULTURA
Generalitat Valenciana https://doi.org/10.13039/501100003359 GRISOLIA%2F2021%2F120
Generalitat Valenciana https://doi.org/10.13039/501100003359 THINKINAZUL%2F2021%2F026
USM USM FRGS%2F1%2F2022%2FSTG03%2FUSM%2F01%2F2
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv The Royal Society Publishing
publisher.none.fl_str_mv The Royal Society Publishing
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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