Large-Scale Movements of IF3 and tRNA during Bacterial Translation Initiation

26 páginas, 7 figuras. Contiene Material suplementario

Bibliographic Details
Authors: Hussain, Tanweer, Llácer, José Luis, Wimberly, Brian T., Kieft, Jeffrey S., Ramakrishnan, V.
Format: article
Status:Published version
Publication Date:2016
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/175906
Online Access:http://hdl.handle.net/10261/175906
Access Level:Open access
Keyword:IF1
IF2
IF3
Cryo-EM
fMet-tRNA
Initiation
Ribosome
Start codon
Structures
Translation
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spelling Large-Scale Movements of IF3 and tRNA during Bacterial Translation InitiationHussain, TanweerLlácer, José LuisWimberly, Brian T.Kieft, Jeffrey S.Ramakrishnan, V.IF1IF2IF3Cryo-EMfMet-tRNAInitiationRibosomeStart codonStructuresTranslation26 páginas, 7 figuras. Contiene Material suplementarioIn bacterial translational initiation, three initiation factors (IFs 1-3) enable the selection of initiator tRNA and the start codon in the P site of the 30S ribosomal subunit. Here, we report 11 single-particle cryo-electron microscopy (cryoEM) reconstructions of the complex of bacterial 30S subunit with initiator tRNA, mRNA, and IFs 1-3, representing different steps along the initiation pathway. IF1 provides key anchoring points for IF2 and IF3, thereby enhancing their activities. IF2 positions a domain in an extended conformation appropriate for capturing the formylmethionyl moiety charged on tRNA. IF3 and tRNA undergo large conformational changes to facilitate the accommodation of the formylmethionyl-tRNA (fMet-tRNA(fMet)) into the P site for start codon recognition.This work was funded by grants to V.R. from the UK Medical Research Council (MC_U105184332), a Wellcome Trust Senior Investigator award (WT096570), the Louis-Jeantet Foundation and the Agouron Institute, and by grants to J.S.K. from HHMI and from the US NIH.Peer reviewedElsevierMedical Research Council (UK)Louis Jeantet FoundationAgouron InstituteNational Institutes of Health (US)Llácer, José Luis [0000-0001-5304-1795]201920192016info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/175906reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.cell.2016.08.074Noinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1759062026-05-22T06:33:51Z
dc.title.none.fl_str_mv Large-Scale Movements of IF3 and tRNA during Bacterial Translation Initiation
title Large-Scale Movements of IF3 and tRNA during Bacterial Translation Initiation
spellingShingle Large-Scale Movements of IF3 and tRNA during Bacterial Translation Initiation
Hussain, Tanweer
IF1
IF2
IF3
Cryo-EM
fMet-tRNA
Initiation
Ribosome
Start codon
Structures
Translation
title_short Large-Scale Movements of IF3 and tRNA during Bacterial Translation Initiation
title_full Large-Scale Movements of IF3 and tRNA during Bacterial Translation Initiation
title_fullStr Large-Scale Movements of IF3 and tRNA during Bacterial Translation Initiation
title_full_unstemmed Large-Scale Movements of IF3 and tRNA during Bacterial Translation Initiation
title_sort Large-Scale Movements of IF3 and tRNA during Bacterial Translation Initiation
dc.creator.none.fl_str_mv Hussain, Tanweer
Llácer, José Luis
Wimberly, Brian T.
Kieft, Jeffrey S.
Ramakrishnan, V.
author Hussain, Tanweer
author_facet Hussain, Tanweer
Llácer, José Luis
Wimberly, Brian T.
Kieft, Jeffrey S.
Ramakrishnan, V.
author_role author
author2 Llácer, José Luis
Wimberly, Brian T.
Kieft, Jeffrey S.
Ramakrishnan, V.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Medical Research Council (UK)
Louis Jeantet Foundation
Agouron Institute
National Institutes of Health (US)
Llácer, José Luis [0000-0001-5304-1795]
dc.subject.none.fl_str_mv IF1
IF2
IF3
Cryo-EM
fMet-tRNA
Initiation
Ribosome
Start codon
Structures
Translation
topic IF1
IF2
IF3
Cryo-EM
fMet-tRNA
Initiation
Ribosome
Start codon
Structures
Translation
description 26 páginas, 7 figuras. Contiene Material suplementario
publishDate 2016
dc.date.none.fl_str_mv 2016
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/175906
url http://hdl.handle.net/10261/175906
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.cell.2016.08.074
No
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869410129717231616
score 15.81155

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