Nicotiana benthamiana resistance to non-adapted Melon necrotic spot virus results from an incompatible interaction between virus RNA and translation initiation factor 4E

10 Pág.

Detalles Bibliográficos
Autores: Nieto García, Cristina, Rodríguez-Moreno, Luis, Rodríguez-Hernández, Ana M., Aranda, Miguel A., Truniger, Verónica
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2011
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/345157
Acceso en línea:http://hdl.handle.net/10261/345157
https://api.elsevier.com/content/abstract/scopus_id/79955383643
Access Level:acceso abierto
Palabra clave:cap-independent translational enhancer
Melon necrotic spot virus
Nicotiana benthamiana
Non-host resistance
Susceptibility factor
Translation initiation factor eIF4E
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spelling Nicotiana benthamiana resistance to non-adapted Melon necrotic spot virus results from an incompatible interaction between virus RNA and translation initiation factor 4ENieto García, CristinaRodríguez-Moreno, LuisRodríguez-Hernández, Ana M.Aranda, Miguel A.Truniger, Verónicacap-independent translational enhancerMelon necrotic spot virusNicotiana benthamianaNon-host resistanceSusceptibility factorTranslation initiation factor eIF4E10 Pág.Nicotiana benthamiana has been described as non-host for Melon necrotic spot virus (MNSV). We investigated the basis of this resistance using the unique opportunity provided by strain MNSV-264, a recombinant virus that is able to overcome the resistance. Analysis of chimeric MNSV mutants showed that virulence in N. benthamiana is conferred by a 49 nucleotide section of the MNSV-264 3'-UTR, which acts in this host as a cap-independent translational enhancer (3'-CITE). Although the 3'-CITE of non-adapted MNSV-Mα5 is active in susceptible melon, it does not promote efficient translation in N. benthamiana, thus preventing expression of proteins required for virus replication. However, MNSV-Mα5 gains the ability to multiply in N. benthamiana cells if eIF4E from a susceptible melon variety (Cm-eIF4E-S) is supplied in trans. These data show that N. benthamiana resistance to MNSV-Mα5 results from incompatibility between the MNSV-Mα5 3'-CITE and N. benthamiana eIF4E in initiating efficient translation of the viral genome. Therefore, non-host resistance conferred by the inability of a host susceptibility factor to support viral multiplication may be a possible mechanism for this type of resistance to viruses.This work was supported by grants AGL2009-07552/AGR (Ministerio de Ciencia e Innovación, Spain) and EUI2009-04009 (PLANT-KBBE2009 program, Ministerio de Ciencia e Innovación, Spain) and 05702/PI/07 Fundación Séneca (Región de Murcia, Spain).Peer reviewedWiley-BlackwellMinisterio de Ciencia e Innovación (España)Fundación SénecaNieto, Cristina [0000-0001-7226-314X]Rodríguez-Moreno, Luis [0000-0003-2385-8782]Truniger, Verónica [0000-0001-8221-2434]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242011info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/345157https://api.elsevier.com/content/abstract/scopus_id/79955383643reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MICINN//AGL2009-07552info:eu-repo/grantAgreement/MICINN//EUI2009-04009The Plant journal : for cell and molecular biologyhttps://doi.org/10.1111/j.1365-313X.2011.04507.xSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3451572026-05-22T06:33:51Z
dc.title.none.fl_str_mv Nicotiana benthamiana resistance to non-adapted Melon necrotic spot virus results from an incompatible interaction between virus RNA and translation initiation factor 4E
title Nicotiana benthamiana resistance to non-adapted Melon necrotic spot virus results from an incompatible interaction between virus RNA and translation initiation factor 4E
spellingShingle Nicotiana benthamiana resistance to non-adapted Melon necrotic spot virus results from an incompatible interaction between virus RNA and translation initiation factor 4E
Nieto García, Cristina
cap-independent translational enhancer
Melon necrotic spot virus
Nicotiana benthamiana
Non-host resistance
Susceptibility factor
Translation initiation factor eIF4E
title_short Nicotiana benthamiana resistance to non-adapted Melon necrotic spot virus results from an incompatible interaction between virus RNA and translation initiation factor 4E
title_full Nicotiana benthamiana resistance to non-adapted Melon necrotic spot virus results from an incompatible interaction between virus RNA and translation initiation factor 4E
title_fullStr Nicotiana benthamiana resistance to non-adapted Melon necrotic spot virus results from an incompatible interaction between virus RNA and translation initiation factor 4E
title_full_unstemmed Nicotiana benthamiana resistance to non-adapted Melon necrotic spot virus results from an incompatible interaction between virus RNA and translation initiation factor 4E
title_sort Nicotiana benthamiana resistance to non-adapted Melon necrotic spot virus results from an incompatible interaction between virus RNA and translation initiation factor 4E
dc.creator.none.fl_str_mv Nieto García, Cristina
Rodríguez-Moreno, Luis
Rodríguez-Hernández, Ana M.
Aranda, Miguel A.
Truniger, Verónica
author Nieto García, Cristina
author_facet Nieto García, Cristina
Rodríguez-Moreno, Luis
Rodríguez-Hernández, Ana M.
Aranda, Miguel A.
Truniger, Verónica
author_role author
author2 Rodríguez-Moreno, Luis
Rodríguez-Hernández, Ana M.
Aranda, Miguel A.
Truniger, Verónica
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Fundación Séneca
Nieto, Cristina [0000-0001-7226-314X]
Rodríguez-Moreno, Luis [0000-0003-2385-8782]
Truniger, Verónica [0000-0001-8221-2434]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv cap-independent translational enhancer
Melon necrotic spot virus
Nicotiana benthamiana
Non-host resistance
Susceptibility factor
Translation initiation factor eIF4E
topic cap-independent translational enhancer
Melon necrotic spot virus
Nicotiana benthamiana
Non-host resistance
Susceptibility factor
Translation initiation factor eIF4E
description 10 Pág.
publishDate 2011
dc.date.none.fl_str_mv 2011
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/345157
https://api.elsevier.com/content/abstract/scopus_id/79955383643
url http://hdl.handle.net/10261/345157
https://api.elsevier.com/content/abstract/scopus_id/79955383643
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MICINN//AGL2009-07552
info:eu-repo/grantAgreement/MICINN//EUI2009-04009
The Plant journal : for cell and molecular biology
https://doi.org/10.1111/j.1365-313X.2011.04507.x
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley-Blackwell
publisher.none.fl_str_mv Wiley-Blackwell
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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