| Resumo: | The full-length nucleotide sequence and genomic organization of a melon necrotic spot virus isolate from Haimen, China (MNSV-HM), were determined. The MNSV-HM genome consists of a positive sense single-stranded RNA, 4267 nt in length, with at least five open reading frames (ORFs) encoding p29, p89, p42, and two small 7-kDa proteins (p7A and p7B). p89 shares a common start codon with p29 and continues through the amber stop codon of p29 to produce an 89-kDa protein. The p7A ORF terminates in an amber codon whose read-through could generate a 14-kDa protein. Phylogenetic analyses based on the p42 amino acid sequence and complete genomic sequence placed MNSV-HM and Spanish isolates of the virus in a major cluster, indicating a close genetic relationship. In conclusion, we report the full-length sequence of MNSV-HM and its translation strategy. The obtained genomic organization and phylogenetic trees indicate that MNSV-HM belongs to the MNSV genus Carmovirus. To the best of our knowledge, this is the first demonstration of the complete nucleotide sequence of an MNSV isolate from China.
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