Supercritical fluid extraction as an alternative process to obtain essential oils with anti-inflammatory properties from marjoram and sweet basil
The anti-inflammatory capacity of supercritical CO2 extracts (S1 and S2) obtained from sage (Salvia officinalis) was evaluated using THP-1 human macrophages activated with human ox-LDL, a specific in vitro model to determine the anti-inflammatory effect of the extracts in an atherosclerotic environm...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2013 |
| País: | España |
| Institución: | Universidad Complutense de Madrid (UCM) |
| Repositorio: | Docta Complutense |
| Idioma: | inglés |
| OAI Identifier: | oai:docta.ucm.es:20.500.14352/92804 |
| Acceso en línea: | https://hdl.handle.net/20.500.14352/92804 |
| Access Level: | acceso abierto |
| Palabra clave: | 663/665 Sage Anti-inflammatory activity Supercritical extracts Anti-inflammatory cytokines Anti-atherogenic model Tecnología de los alimentos 3309 Tecnología de Los Alimentos |
| Sumario: | The anti-inflammatory capacity of supercritical CO2 extracts (S1 and S2) obtained from sage (Salvia officinalis) was evaluated using THP-1 human macrophages activated with human ox-LDL, a specific in vitro model to determine the anti-inflammatory effect of the extracts in an atherosclerotic environment. The expression of pro-inflammatory cytokines, with an important role in the atherogenic process, such as TNF-α, IL-1β and IL-6, in presence of different extracts concentrations was evaluated. Results showed that 30 μg/mL of both supercritical extracts (S1 and S2) markedly suppressed the ox-LDL induced production of TNF-α, IL-1β and IL-6, as well as their mRNA expression. Data showed that S1 presented a higher anti-inflammatory activity than S2. A characterization by GC–MS of sage extracts identified 16 compounds, mainly camphor, borneol and 1,8-cineole. These three compounds represented a 62.4% of S1 and a 48.1% of S2. Camphor, borneol and 1,8-cineole presented an important anti-inflammatory activity in the proposed model, with a decrease in the release and gene expression of TNF-α, IL-1β and IL-6 and an increase in IL-10 expression. These results explained the higher activity found in S1. This study suggested that supercritical sage extracts could be used as food ingredients in the development of anti-inflammatory/anti-atherogenic products. |
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