Interplay of walnut consumption, changes in circulating miRNAs and reduction in LDL-cholesterol in elders

The mechanisms underlying the lipid-lowering effect of nuts remain elusive. This study explores whether one-year supplementation with walnuts decreases LDL-cholesterol (LDL-C) by affecting the expression of circulating microRNAs (c-miRNA). In this sub-study of the Walnuts and Healthy Aging (WAHA) tr...

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Autores: Gil-Zamorano, Judit, Cofán, Montserrat, López de las Hazas, María del Carmen, García-Blanco, Tatiana, García-Ruiz, Almudena, Doménech, Mónica, Serra-Mir, Mercè, Roth, Irene, Valls Pedret, Cinta, Rajaram, Sujatha, Sabaté, Joan, Ros, Emilio, Dávalos, Alberto, Sala Vila, Aleix
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Universitat Pompeu Fabra
Repositorio:Repositorio Digital de la UPF
OAI Identifier:oai:repositori.upf.edu:10230/54362
Acceso en línea:http://hdl.handle.net/10230/54362
http://dx.doi.org/10.3390/nu14071473
Access Level:acceso abierto
Palabra clave:Alpha-linolenic acid
Biomarkers
Cholesterol
Lipid metabolism
Nuts
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spelling Interplay of walnut consumption, changes in circulating miRNAs and reduction in LDL-cholesterol in eldersGil-Zamorano, JuditCofán, MontserratLópez de las Hazas, María del CarmenGarcía-Blanco, TatianaGarcía-Ruiz, AlmudenaDoménech, MónicaSerra-Mir, MercèRoth, IreneValls Pedret, CintaRajaram, SujathaSabaté, JoanRos, EmilioDávalos, AlbertoSala Vila, AleixAlpha-linolenic acidBiomarkersCholesterolLipid metabolismNutsThe mechanisms underlying the lipid-lowering effect of nuts remain elusive. This study explores whether one-year supplementation with walnuts decreases LDL-cholesterol (LDL-C) by affecting the expression of circulating microRNAs (c-miRNA). In this sub-study of the Walnuts and Healthy Aging (WAHA) trial, we obtained fasting serum at baseline and at 1 year from 330 free-living participants (63-79 year, 68% women), allocated into a control group (CG, abstinence from walnuts, n = 164) and a walnut group (WG, 15% of daily energy as walnuts, ~30-60 g/d, n = 166). Participants in the WG showed a 1 year decrease in LDL-C (-9.07, (95% confidence interval: -12.87; -5.73) mg/dL; p = 0.010 versus changes in the CG). We conducted a miRNA array in eight randomly selected participants in the WG who decreased in LDL-C. This yielded 53 c-miRNAs with statistically significant changes, 27 of which survived the correction for multiple testing. When validating them in the full population, statistical significance lasted for hsa-miR-551a, being upregulated in the WG. In mediation analysis, the change in hsa-miR-551a was unrelated to LDL-C decrease. Long-term supplementation with walnuts decreased LDL-C independently of the changes in c-miRNA. The hsa-miR-551a upregulation, which has been linked to a reduced cell migration and invasion in several carcinomas, suggests a novel mechanism of walnuts in cancer risk.This work was supported by a grant from the California Walnut Commission, Folsom, CA, US (to J.S. and E.R.), by a “Manuel de Hoya” grant (2014) from the Sociedad Española de Arteriosclerosis, and by Fondo de Investigación Sanitaria–Fondo Europeo de Desarrollo Regional (grant no. PI15/01014) (all to A.S.-V.). M.-C.L.d.l.H. was supported by a post-doctoral research contract funded by the community of Madrid and the European Union (PEJD-2016/BIO-2781). A.G.-R. acknowledges the Marie Curie AMAROUT-II Europe Program. A.D. was supported by the Spanish Agencia Estatal de Investigación and European Feder Funds (AGL2016-78922-R) and Fundación Ramón Areces (CIVP18A3888), Madrid, Spain. These funding agencies had no input in the study design, data collection, analyses, or writing and submission of the manuscript.MDPI202220222022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/54362http://dx.doi.org/10.3390/nu14071473reponame:Repositorio Digital de la UPFinstname:Universitat Pompeu FabraInglésNutrients. 2022 Apr 1;14(7):1473info:eu-repo/grantAgreement/ES/1PE/AGL2016-78922-R© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repositori.upf.edu:10230/543622026-06-12T07:21:37Z
dc.title.none.fl_str_mv Interplay of walnut consumption, changes in circulating miRNAs and reduction in LDL-cholesterol in elders
title Interplay of walnut consumption, changes in circulating miRNAs and reduction in LDL-cholesterol in elders
spellingShingle Interplay of walnut consumption, changes in circulating miRNAs and reduction in LDL-cholesterol in elders
Gil-Zamorano, Judit
Alpha-linolenic acid
Biomarkers
Cholesterol
Lipid metabolism
Nuts
title_short Interplay of walnut consumption, changes in circulating miRNAs and reduction in LDL-cholesterol in elders
title_full Interplay of walnut consumption, changes in circulating miRNAs and reduction in LDL-cholesterol in elders
title_fullStr Interplay of walnut consumption, changes in circulating miRNAs and reduction in LDL-cholesterol in elders
title_full_unstemmed Interplay of walnut consumption, changes in circulating miRNAs and reduction in LDL-cholesterol in elders
title_sort Interplay of walnut consumption, changes in circulating miRNAs and reduction in LDL-cholesterol in elders
dc.creator.none.fl_str_mv Gil-Zamorano, Judit
Cofán, Montserrat
López de las Hazas, María del Carmen
García-Blanco, Tatiana
García-Ruiz, Almudena
Doménech, Mónica
Serra-Mir, Mercè
Roth, Irene
Valls Pedret, Cinta
Rajaram, Sujatha
Sabaté, Joan
Ros, Emilio
Dávalos, Alberto
Sala Vila, Aleix
author Gil-Zamorano, Judit
author_facet Gil-Zamorano, Judit
Cofán, Montserrat
López de las Hazas, María del Carmen
García-Blanco, Tatiana
García-Ruiz, Almudena
Doménech, Mónica
Serra-Mir, Mercè
Roth, Irene
Valls Pedret, Cinta
Rajaram, Sujatha
Sabaté, Joan
Ros, Emilio
Dávalos, Alberto
Sala Vila, Aleix
author_role author
author2 Cofán, Montserrat
López de las Hazas, María del Carmen
García-Blanco, Tatiana
García-Ruiz, Almudena
Doménech, Mónica
Serra-Mir, Mercè
Roth, Irene
Valls Pedret, Cinta
Rajaram, Sujatha
Sabaté, Joan
Ros, Emilio
Dávalos, Alberto
Sala Vila, Aleix
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Alpha-linolenic acid
Biomarkers
Cholesterol
Lipid metabolism
Nuts
topic Alpha-linolenic acid
Biomarkers
Cholesterol
Lipid metabolism
Nuts
description The mechanisms underlying the lipid-lowering effect of nuts remain elusive. This study explores whether one-year supplementation with walnuts decreases LDL-cholesterol (LDL-C) by affecting the expression of circulating microRNAs (c-miRNA). In this sub-study of the Walnuts and Healthy Aging (WAHA) trial, we obtained fasting serum at baseline and at 1 year from 330 free-living participants (63-79 year, 68% women), allocated into a control group (CG, abstinence from walnuts, n = 164) and a walnut group (WG, 15% of daily energy as walnuts, ~30-60 g/d, n = 166). Participants in the WG showed a 1 year decrease in LDL-C (-9.07, (95% confidence interval: -12.87; -5.73) mg/dL; p = 0.010 versus changes in the CG). We conducted a miRNA array in eight randomly selected participants in the WG who decreased in LDL-C. This yielded 53 c-miRNAs with statistically significant changes, 27 of which survived the correction for multiple testing. When validating them in the full population, statistical significance lasted for hsa-miR-551a, being upregulated in the WG. In mediation analysis, the change in hsa-miR-551a was unrelated to LDL-C decrease. Long-term supplementation with walnuts decreased LDL-C independently of the changes in c-miRNA. The hsa-miR-551a upregulation, which has been linked to a reduced cell migration and invasion in several carcinomas, suggests a novel mechanism of walnuts in cancer risk.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/54362
http://dx.doi.org/10.3390/nu14071473
url http://hdl.handle.net/10230/54362
http://dx.doi.org/10.3390/nu14071473
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Nutrients. 2022 Apr 1;14(7):1473
info:eu-repo/grantAgreement/ES/1PE/AGL2016-78922-R
dc.rights.none.fl_str_mv https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositorio Digital de la UPF
instname:Universitat Pompeu Fabra
instname_str Universitat Pompeu Fabra
reponame_str Repositorio Digital de la UPF
collection Repositorio Digital de la UPF
repository.name.fl_str_mv
repository.mail.fl_str_mv
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