Evaluation of immunomodulatory properties of phenolic extracts from olive mill by-products using Caco-2 cells and molecular docking analysis
The bioactive potential of phenolic extracts derived from olive mill solid by-product (OMSbP), also called alperujo, and olive mill water (OMW), remains a topic of significant interest due to the amount of these, espe- cially in Mediterranean regions, and their potential health benefits. In this stu...
| Autores: | , , , , , |
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| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2024 |
| País: | España |
| Recursos: | Universidad de Sevilla (US) |
| Repositorio: | idUS. Depósito de Investigación de la Universidad de Sevilla |
| OAI Identifier: | oai:idus.us.es:11441/168962 |
| Acesso em linha: | https://hdl.handle.net/11441/168962 https://doi.org/10.1016/j.jafr.2024.101399 |
| Access Level: | acceso abierto |
| Palavra-chave: | Olive oil Olea europea Hydroxytyrosol By-products Immunonutrition |
| Resumo: | The bioactive potential of phenolic extracts derived from olive mill solid by-product (OMSbP), also called alperujo, and olive mill water (OMW), remains a topic of significant interest due to the amount of these, espe- cially in Mediterranean regions, and their potential health benefits. In this study, the antioxidant and immu- nomodulatory properties of phenolic extracts obtained from OMSbP and OMW (OMSbP-P and OMW-P, respectively) in intestinal cells were evaluated. Initially, phenol-rich extracts were prepared by ethanol/ethyl acetate extraction and were characterized by HPLC-DAD analyses. The in vitro antioxidant activity showed potent bioactivities for all extracts evaluated. Toxicity assays did not reveal adverse effects on Caco-2 cell viability at concentrations relevant to immunomodulatory activity. Subsequently, the immunomodulatory effects of these extracts were assessed by measuring gene expression of pro- and anti-inflammatory cytokines. The results demonstrate that both OMSbP-P and OMW-P exhibit significant immunomodulatory activities, as evidenced by modulated cytokine gene expression in intestinal cells. Furthermore, molecular docking analyses of the identified phenols with the TLR4/MD2 receptor showed binding affinity, increasing the evidence that these extracts could exert immunomodulatory activity. Overall, our findings suggest that OMSbP-P and OMW-P possess promising immunomodulatory properties in intestinal cells, thus emphasizing their potential as natural therapeutic agents for managing immune-related disorders and promoting gut health. Further elucidation of the molecular mech- anisms underlying these effects and in vivo studies are warranted to fully exploit the therapeutic potential of these extracts. |
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