Investigating the role of Gli2 in mouse embryonic stem cells and mouse embryogenesis

Embryonic stem cells (ESCs) are derived from the inner cell mass of the pre-implantation embryo and can recapitulate early developmental stages of the embryo. ESCs have the ability to continuously propagate while maintaining the capacity to differentiate into all cell lineages of the epiblast. The W...

Descripción completa

Detalles Bibliográficos
Autor: Tu, Xiaochuan
Tipo de recurso: tesis doctoral
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:CBUC, CESCA
Repositorio:TDR. Tesis Doctorales en Red
OAI Identifier:oai:www.tdx.cat:10803/688952
Acceso en línea:http://hdl.handle.net/10803/688952
Access Level:acceso abierto
Palabra clave:Transcription factor
Embryonic stem cell
Embryogenesis
Pluripotency
Differentiation
Factor de transcripcion
Células madre embrionarias
Embriogénesis
Pluripotencialidad
Diferenciación
576
id ES_613a96a3a10a712872bf3919c6c19b76
oai_identifier_str oai:www.tdx.cat:10803/688952
network_acronym_str ES
network_name_str España
repository_id_str
spelling Investigating the role of Gli2 in mouse embryonic stem cells and mouse embryogenesisTu, XiaochuanTranscription factorEmbryonic stem cellEmbryogenesisPluripotencyDifferentiationFactor de transcripcionCélulas madre embrionariasEmbriogénesisPluripotencialidadDiferenciación576Embryonic stem cells (ESCs) are derived from the inner cell mass of the pre-implantation embryo and can recapitulate early developmental stages of the embryo. ESCs have the ability to continuously propagate while maintaining the capacity to differentiate into all cell lineages of the epiblast. The Wnt/ β-catenin signalling pathway plays a fundamental role in controlling the mouse ESC regulatory circuit. However, the downstream targets of the Wnt pathway contributing to the process of pluripotency remain unknown. Here we used computational analysis to identify Gli2 as a putative downstream target of Wnt, a regulator of mESC pluripotency and a potential regulator of early embryo development. Investigating the role of this factor gave us further insight into ESC and embryo biology.Les cèl·lules mare embrionàries (ESC) es deriven de la massa cel·lular interna de la preimplantació embrionària i poden recapitular les primeres etapes del desenvolupament embrionari. Els ESC tenen la capacitat de propagar-se contínuament, mantenint la capacitat de diferenciar-se en tots els llinatges cel·lulars de l'epiblast. La via de senyalització Wnt / β-catenin té un paper fonamental en el control del circuit regulador ESC del ratolí, tanmateix, els objectius de la via Wnt que contribueixen al procés de pluripotència encara són desconeguts. Aquí, mitjançant l’anàlisi computacional, vam identificar un objectiu putatiu Wnt Gli2 com un regulador de la pluripotència de mESC i un regulador potencial en el desenvolupament primerenc d'embrions. Investigar el paper d'aquest factor ens va donar una visió de l'ESC i la biologia de l'embrió.Programa de Doctorat en BiomedicinaUniversitat Pompeu FabraCosma, Maria PiaUniversitat Pompeu Fabra. Departament de Medicina i Ciències de la Vida202320252023info:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/publishedVersion162 p.application/pdfhttp://hdl.handle.net/10803/688952TDX (Tesis Doctorals en Xarxa)reponame:TDR. Tesis Doctorales en Redinstname:CBUC, CESCAInglésL'accés als continguts d'aquesta tesi queda condicionat a l'acceptació de les condicions d'ús establertes per la següent llicència Creative Commons: http://creativecommons.org/licenses/by-nc/4.0/http://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessoai:www.tdx.cat:10803/6889522026-06-14T12:46:07Z
dc.title.none.fl_str_mv Investigating the role of Gli2 in mouse embryonic stem cells and mouse embryogenesis
title Investigating the role of Gli2 in mouse embryonic stem cells and mouse embryogenesis
spellingShingle Investigating the role of Gli2 in mouse embryonic stem cells and mouse embryogenesis
Tu, Xiaochuan
Transcription factor
Embryonic stem cell
Embryogenesis
Pluripotency
Differentiation
Factor de transcripcion
Células madre embrionarias
Embriogénesis
Pluripotencialidad
Diferenciación
576
title_short Investigating the role of Gli2 in mouse embryonic stem cells and mouse embryogenesis
title_full Investigating the role of Gli2 in mouse embryonic stem cells and mouse embryogenesis
title_fullStr Investigating the role of Gli2 in mouse embryonic stem cells and mouse embryogenesis
title_full_unstemmed Investigating the role of Gli2 in mouse embryonic stem cells and mouse embryogenesis
title_sort Investigating the role of Gli2 in mouse embryonic stem cells and mouse embryogenesis
dc.creator.none.fl_str_mv Tu, Xiaochuan
author Tu, Xiaochuan
author_facet Tu, Xiaochuan
author_role author
dc.contributor.none.fl_str_mv Cosma, Maria Pia
Universitat Pompeu Fabra. Departament de Medicina i Ciències de la Vida
dc.subject.none.fl_str_mv Transcription factor
Embryonic stem cell
Embryogenesis
Pluripotency
Differentiation
Factor de transcripcion
Células madre embrionarias
Embriogénesis
Pluripotencialidad
Diferenciación
576
topic Transcription factor
Embryonic stem cell
Embryogenesis
Pluripotency
Differentiation
Factor de transcripcion
Células madre embrionarias
Embriogénesis
Pluripotencialidad
Diferenciación
576
description Embryonic stem cells (ESCs) are derived from the inner cell mass of the pre-implantation embryo and can recapitulate early developmental stages of the embryo. ESCs have the ability to continuously propagate while maintaining the capacity to differentiate into all cell lineages of the epiblast. The Wnt/ β-catenin signalling pathway plays a fundamental role in controlling the mouse ESC regulatory circuit. However, the downstream targets of the Wnt pathway contributing to the process of pluripotency remain unknown. Here we used computational analysis to identify Gli2 as a putative downstream target of Wnt, a regulator of mESC pluripotency and a potential regulator of early embryo development. Investigating the role of this factor gave us further insight into ESC and embryo biology.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/doctoralThesis
info:eu-repo/semantics/publishedVersion
format doctoralThesis
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10803/688952
url http://hdl.handle.net/10803/688952
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 162 p.
application/pdf
dc.publisher.none.fl_str_mv Universitat Pompeu Fabra
publisher.none.fl_str_mv Universitat Pompeu Fabra
dc.source.none.fl_str_mv TDX (Tesis Doctorals en Xarxa)
reponame:TDR. Tesis Doctorales en Red
instname:CBUC, CESCA
instname_str CBUC, CESCA
reponame_str TDR. Tesis Doctorales en Red
collection TDR. Tesis Doctorales en Red
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869409389175111680
score 15.300719

Similares en LA Referencia