A covalently linked probe to monitor local membrane properties surrounding plasma membrane proteins

Functional membrane proteins in the plasma membrane are suggested to have specific membrane environments that play important roles to maintain and regulate their function. However, the local membrane environments of membrane proteins remain largely unexplored due to the lack of available techniques....

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Detalles Bibliográficos
Autores: Umebayashi, Miwa, Takemoto, Satoko, Reymond, Luc, Sundukova, Mayya, Hovius, Ruud, Bucci, Annalisa, Heppenstall, Paul A., Yokota, Hideo, Johnsson, Kai, Riezman, Howard
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/339032
Acceso en línea:http://hdl.handle.net/10261/339032
https://api.elsevier.com/content/abstract/scopus_id/85144809386
Access Level:acceso abierto
Palabra clave:Biochemistry
Membrane and lipid biology
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spelling A covalently linked probe to monitor local membrane properties surrounding plasma membrane proteinsUmebayashi, MiwaTakemoto, SatokoReymond, LucSundukova, MayyaHovius, RuudBucci, AnnalisaHeppenstall, Paul A.Yokota, HideoJohnsson, KaiRiezman, HowardBiochemistryMembrane and lipid biologyFunctional membrane proteins in the plasma membrane are suggested to have specific membrane environments that play important roles to maintain and regulate their function. However, the local membrane environments of membrane proteins remain largely unexplored due to the lack of available techniques. We have developed a method to probe the local membrane environment surrounding membrane proteins in the plasma membrane by covalently tethering a solvatochromic, environment-sensitive dye, Nile Red, to a GPI-anchored protein and the insulin receptor through a flexible linker. The fluidity of the membrane environment of the GPI-anchored protein depended upon the saturation of the acyl chains of the lipid anchor. The local environment of the insulin receptor was distinct from the average plasma membrane fluidity and was quite dynamic and heterogeneous. Upon addition of insulin, the local membrane environment surrounding the receptor specifically increased in fluidity in an insulin receptor-kinase dependent manner and on the distance between the dye and the receptor.This work was funded by the NCCR Chemical Biology, the Swiss National Science Foundation (grants 51NF40_185898, 310030, and _184949), and the Leducq Foundation.Peer reviewedRockefeller University PressNational Centres of Competence in Research (Switzerland)Swiss National Science FoundationFondation LeducqUmebayashi, Miwa [0000-0002-5873-8417]Takemoto, Satoko [0000-0003-0258-6589]Reymond, Luc [0000-0001-5282-9273]Sundukova, Mayya [0000-0003-1328-0008]Hovius, Ruud [0000-0001-9258-6587]Bucci, Annalisa [0000-0002-6955-2204]Heppenstall, Paul A. [0000-0001-7954-5166]Yokota, Hideo [0000-0003-1395-309X]Johnsson, Kai [0000-0002-8002-1981]Riezman, Howard [0000-0003-4680-9422]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/339032https://api.elsevier.com/content/abstract/scopus_id/85144809386reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésThe underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1083/jcb.202206119https://doi.org/10.1083/jcb.202206119Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3390322026-05-22T06:33:51Z
dc.title.none.fl_str_mv A covalently linked probe to monitor local membrane properties surrounding plasma membrane proteins
title A covalently linked probe to monitor local membrane properties surrounding plasma membrane proteins
spellingShingle A covalently linked probe to monitor local membrane properties surrounding plasma membrane proteins
Umebayashi, Miwa
Biochemistry
Membrane and lipid biology
title_short A covalently linked probe to monitor local membrane properties surrounding plasma membrane proteins
title_full A covalently linked probe to monitor local membrane properties surrounding plasma membrane proteins
title_fullStr A covalently linked probe to monitor local membrane properties surrounding plasma membrane proteins
title_full_unstemmed A covalently linked probe to monitor local membrane properties surrounding plasma membrane proteins
title_sort A covalently linked probe to monitor local membrane properties surrounding plasma membrane proteins
dc.creator.none.fl_str_mv Umebayashi, Miwa
Takemoto, Satoko
Reymond, Luc
Sundukova, Mayya
Hovius, Ruud
Bucci, Annalisa
Heppenstall, Paul A.
Yokota, Hideo
Johnsson, Kai
Riezman, Howard
author Umebayashi, Miwa
author_facet Umebayashi, Miwa
Takemoto, Satoko
Reymond, Luc
Sundukova, Mayya
Hovius, Ruud
Bucci, Annalisa
Heppenstall, Paul A.
Yokota, Hideo
Johnsson, Kai
Riezman, Howard
author_role author
author2 Takemoto, Satoko
Reymond, Luc
Sundukova, Mayya
Hovius, Ruud
Bucci, Annalisa
Heppenstall, Paul A.
Yokota, Hideo
Johnsson, Kai
Riezman, Howard
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv National Centres of Competence in Research (Switzerland)
Swiss National Science Foundation
Fondation Leducq
Umebayashi, Miwa [0000-0002-5873-8417]
Takemoto, Satoko [0000-0003-0258-6589]
Reymond, Luc [0000-0001-5282-9273]
Sundukova, Mayya [0000-0003-1328-0008]
Hovius, Ruud [0000-0001-9258-6587]
Bucci, Annalisa [0000-0002-6955-2204]
Heppenstall, Paul A. [0000-0001-7954-5166]
Yokota, Hideo [0000-0003-1395-309X]
Johnsson, Kai [0000-0002-8002-1981]
Riezman, Howard [0000-0003-4680-9422]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Biochemistry
Membrane and lipid biology
topic Biochemistry
Membrane and lipid biology
description Functional membrane proteins in the plasma membrane are suggested to have specific membrane environments that play important roles to maintain and regulate their function. However, the local membrane environments of membrane proteins remain largely unexplored due to the lack of available techniques. We have developed a method to probe the local membrane environment surrounding membrane proteins in the plasma membrane by covalently tethering a solvatochromic, environment-sensitive dye, Nile Red, to a GPI-anchored protein and the insulin receptor through a flexible linker. The fluidity of the membrane environment of the GPI-anchored protein depended upon the saturation of the acyl chains of the lipid anchor. The local environment of the insulin receptor was distinct from the average plasma membrane fluidity and was quite dynamic and heterogeneous. Upon addition of insulin, the local membrane environment surrounding the receptor specifically increased in fluidity in an insulin receptor-kinase dependent manner and on the distance between the dye and the receptor.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/339032
https://api.elsevier.com/content/abstract/scopus_id/85144809386
url http://hdl.handle.net/10261/339032
https://api.elsevier.com/content/abstract/scopus_id/85144809386
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1083/jcb.202206119
https://doi.org/10.1083/jcb.202206119
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Rockefeller University Press
publisher.none.fl_str_mv Rockefeller University Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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