Generation and characterization of human iPSC line generated from mesenchymal stem cells derived from adipose tissue

Abstract In this work, mesenchymal stem cells derived from adipose tissue (ADSCs) were used for the generation of the human-induced pluripotent stem cell line G15.AO. Cell reprogramming was performed using retroviral vectors containing the Yamanaka factors, and the generated G15.AO hiPSC line showed...

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Detalhes bibliográficos
Autores: Rodriguez-Madoz, J.R. (Juan Roberto)|||/items/9c21019b-aeba-4cdc-b78e-178a176da535, Prosper-Cardoso, F. (Felipe)|||/items/3d1b0b82-06c3-4e63-8280-e903dc4dc0c1, Barajas, M. (Miguel)|||/items/9b7ddee9-f983-4f54-b9a1-0355ab9c8080, Andreu, E.J. (Enrique José)|||/items/bfb7adec-1ed3-4313-a2a7-8b026c48eba6, Abizanda-Sarasa, G. (Gloria)|||/items/1e76a0e3-32d0-424e-b58a-d9ddf7a82ef7, Mazo-Vega, M.M. (Manuel María)|||/items/c871e8d9-ee5d-487b-97ef-7302feb8a02e, Rodriguez, S. (Saray)|||/items/c707d2b6-1137-4320-915b-4ffd88ad86f2, Zapata-Linares, N.M. (Natalia María)|||/items/84c37591-5f83-4b46-baae-9cf1720a817e
Tipo de documento: artigo
Data de publicação:2016
País:España
Recursos:Universidad de Navarra
Repositório:Dadun. Depósito Académico Digital de la Universidad de Navarra
Idioma:inglês
OAI Identifier:oai:dadun.unav.edu:10171/39951
Acesso em linha:https://hdl.handle.net/10171/39951
Access Level:Acceso aberto
Palavra-chave:Materias Investigacion::Ciencias de la Salud::Microbiología y biología molecular
Materias Investigacion::Ciencias de la Salud::Oncología
Mesenchymal stem cells
Adipose tissue
Human iPSC line
Stem cell
Área de Terapia Celular
Descrição
Resumo:Abstract In this work, mesenchymal stem cells derived from adipose tissue (ADSCs) were used for the generation of the human-induced pluripotent stem cell line G15.AO. Cell reprogramming was performed using retroviral vectors containing the Yamanaka factors, and the generated G15.AO hiPSC line showed normal karyotype, silencing of the exogenous reprogramming factors, induction of the typical pluripotency-associated markers, alkaline phosphatase enzymatic activity, and in vivo and in vitro differentiation ability to the three germ layers.

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