Synaptic Clusters of MHC Class II Molecules Induced on DCs by Adhesion Molecule–mediated Initial T-Cell Scanning

Initial adhesive contacts between T lymphocytes and dendritic cells (DCs) facilitate recognition of peptide-MHC complexes by the TCR. In this report, we studied the dynamic behavior of adhesion and Ag receptors on DCs during initial contacts with T-cells. Adhesion molecules LFA-1- and ICAM-1,3-GFP a...

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Detalles Bibliográficos
Autores: Fuente, Hortensia de la, Mittelbrunn, María, Sánchez-Martín, Lorena, Vicente-Manzanares, Miguel, Lamana Domínguez, Amalia, Pardi, Ruggero, Cabañas Gutiérrez, Carlos, Sánchez-Madrid, Francisco
Tipo de recurso: artículo
Fecha de publicación:2005
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/93998
Acceso en línea:https://hdl.handle.net/20.500.14352/93998
Access Level:acceso abierto
Palabra clave:576.3
577.27
T-cell
Synaptic Clusters
MHC Class II Molecules
Biología celular (Biología)
Inmunología
2412 Inmunología
2407 Biología Celular
id ES_522a0dedcb763cc2a17b0e8db40b46ed
oai_identifier_str oai:docta.ucm.es:20.500.14352/93998
network_acronym_str ES
network_name_str España
repository_id_str
spelling Synaptic Clusters of MHC Class II Molecules Induced on DCs by Adhesion Molecule–mediated Initial T-Cell ScanningFuente, Hortensia de laMittelbrunn, MaríaSánchez-Martín, LorenaVicente-Manzanares, MiguelLamana Domínguez, AmaliaPardi, RuggeroCabañas Gutiérrez, CarlosSánchez-Madrid, Francisco576.3577.27T-cellSynaptic ClustersMHC Class II MoleculesBiología celular (Biología)Inmunología2412 Inmunología2407 Biología CelularInitial adhesive contacts between T lymphocytes and dendritic cells (DCs) facilitate recognition of peptide-MHC complexes by the TCR. In this report, we studied the dynamic behavior of adhesion and Ag receptors on DCs during initial contacts with T-cells. Adhesion molecules LFA-1- and ICAM-1,3-GFP as well as MHC class II-GFP molecules were very rapidly concentrated at the DC contact area. Binding of ICAM-3, and ICAM-1 to a lesser extent, to LFA-1 expressed by mature but not immature DC, induced MHC-II clustering into the immune synapse. Also, ICAM-3 binding to DC induced the activation of the Vav1-Rac1 axis, a regulatory pathway involved in actin cytoskeleton reorganization, which was essential for MHC-II clustering on DCs. Our results support a model in which ICAM-mediated MHC-II clustering on DC constitutes a priming mechanism to enhance antigen presentation to T-cells.American Society for Cell BiologyUniversidad Complutense de Madrid20052005-01-0120052005-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/93998reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/939982026-06-02T12:44:21Z
dc.title.none.fl_str_mv Synaptic Clusters of MHC Class II Molecules Induced on DCs by Adhesion Molecule–mediated Initial T-Cell Scanning
title Synaptic Clusters of MHC Class II Molecules Induced on DCs by Adhesion Molecule–mediated Initial T-Cell Scanning
spellingShingle Synaptic Clusters of MHC Class II Molecules Induced on DCs by Adhesion Molecule–mediated Initial T-Cell Scanning
Fuente, Hortensia de la
576.3
577.27
T-cell
Synaptic Clusters
MHC Class II Molecules
Biología celular (Biología)
Inmunología
2412 Inmunología
2407 Biología Celular
title_short Synaptic Clusters of MHC Class II Molecules Induced on DCs by Adhesion Molecule–mediated Initial T-Cell Scanning
title_full Synaptic Clusters of MHC Class II Molecules Induced on DCs by Adhesion Molecule–mediated Initial T-Cell Scanning
title_fullStr Synaptic Clusters of MHC Class II Molecules Induced on DCs by Adhesion Molecule–mediated Initial T-Cell Scanning
title_full_unstemmed Synaptic Clusters of MHC Class II Molecules Induced on DCs by Adhesion Molecule–mediated Initial T-Cell Scanning
title_sort Synaptic Clusters of MHC Class II Molecules Induced on DCs by Adhesion Molecule–mediated Initial T-Cell Scanning
dc.creator.none.fl_str_mv Fuente, Hortensia de la
Mittelbrunn, María
Sánchez-Martín, Lorena
Vicente-Manzanares, Miguel
Lamana Domínguez, Amalia
Pardi, Ruggero
Cabañas Gutiérrez, Carlos
Sánchez-Madrid, Francisco
author Fuente, Hortensia de la
author_facet Fuente, Hortensia de la
Mittelbrunn, María
Sánchez-Martín, Lorena
Vicente-Manzanares, Miguel
Lamana Domínguez, Amalia
Pardi, Ruggero
Cabañas Gutiérrez, Carlos
Sánchez-Madrid, Francisco
author_role author
author2 Mittelbrunn, María
Sánchez-Martín, Lorena
Vicente-Manzanares, Miguel
Lamana Domínguez, Amalia
Pardi, Ruggero
Cabañas Gutiérrez, Carlos
Sánchez-Madrid, Francisco
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 576.3
577.27
T-cell
Synaptic Clusters
MHC Class II Molecules
Biología celular (Biología)
Inmunología
2412 Inmunología
2407 Biología Celular
topic 576.3
577.27
T-cell
Synaptic Clusters
MHC Class II Molecules
Biología celular (Biología)
Inmunología
2412 Inmunología
2407 Biología Celular
description Initial adhesive contacts between T lymphocytes and dendritic cells (DCs) facilitate recognition of peptide-MHC complexes by the TCR. In this report, we studied the dynamic behavior of adhesion and Ag receptors on DCs during initial contacts with T-cells. Adhesion molecules LFA-1- and ICAM-1,3-GFP as well as MHC class II-GFP molecules were very rapidly concentrated at the DC contact area. Binding of ICAM-3, and ICAM-1 to a lesser extent, to LFA-1 expressed by mature but not immature DC, induced MHC-II clustering into the immune synapse. Also, ICAM-3 binding to DC induced the activation of the Vav1-Rac1 axis, a regulatory pathway involved in actin cytoskeleton reorganization, which was essential for MHC-II clustering on DCs. Our results support a model in which ICAM-mediated MHC-II clustering on DC constitutes a priming mechanism to enhance antigen presentation to T-cells.
publishDate 2005
dc.date.none.fl_str_mv 2005
2005-01-01
2005
2005-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/93998
url https://hdl.handle.net/20.500.14352/93998
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Cell Biology
publisher.none.fl_str_mv American Society for Cell Biology
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869408013168672768
score 15.811543

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