Identification of the major ACE-inhibitory peptides produced by Eenzymatic hydrolysis of a protein concentrate from cuttlefish wastewater

16 páginas, 2 tablas, 4 figuras.-- This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/)

Detalles Bibliográficos
Autores: Rodríguez-Amado, I., Vázquez, José Antonio, González Fernández, Pilar, Esteban-Fernández, Diego, Carrera, Mónica, Piñeiro, Carmen
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/102893
Acceso en línea:http://hdl.handle.net/10261/102893
Access Level:acceso abierto
Palabra clave:Ultrafiltration
Proteolysis
ACE inhibitory peptides
Cuttlefish byproducts
Peptide identification
HPLC-ESI-MS
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spelling Identification of the major ACE-inhibitory peptides produced by Eenzymatic hydrolysis of a protein concentrate from cuttlefish wastewaterRodríguez-Amado, I.Vázquez, José AntonioGonzález Fernández, PilarEsteban-Fernández, DiegoCarrera, MónicaPiñeiro, CarmenUltrafiltrationProteolysisACE inhibitory peptidesCuttlefish byproductsPeptide identificationHPLC-ESI-MS16 páginas, 2 tablas, 4 figuras.-- This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/)The aim of this work was the purification and identification of the major angiotensin converting enzyme (ACE) inhibitory peptides produced by enzymatic hydrolysis of a protein concentrate recovered from a cuttlefish industrial manufacturing effluent. This process consisted on the ultrafiltration of cuttlefish softening wastewater, with a 10 kDa cut-off membrane, followed by the hydrolysis with alcalase of the retained fraction. Alcalase produced ACE inhibitors reaching the highest activity (IC50 = 76.8 ± 15.2 μg mL−1) after 8 h of proteolysis. Sequential ultrafiltration of the 8 h hydrolysate with molecular weight cut-off (MWCO) membranes of 10 and 1 kDa resulted in the increased activity of each permeate, with a final IC50 value of 58.4 ± 4.6 μg mL−1. Permeate containing peptides lower than 1 kDa was separated by reversed-phase high performance liquid chromatography (RP-HPLC). Four fractions (A–D) with potent ACE inhibitory activity were isolated and their main peptides identified using high performance liquid chromatography coupled to an electrospray ion trap Fourier transform ion cyclotron resonance-mass spectrometer (HPLC-ESI-IT-FTICR) followed by comparison with databases and de novo sequencing. The amino acid sequences of the identified peptides contained at least one hydrophobic and/or a proline together with positively charged residues in at least one of the three C-terminal positions. The IC50 values of the fractions ranged from 1.92 to 8.83 μg mL−1, however this study fails to identify which of these peptides are ultimately responsible for the potent antihypertensive activity of these fractionsThe company Frinova S.A. funded this study (Contract N° 20090732 co-financed by CDTI). The authors also thank the Unit of Information Resources for Research (URICI-CSIC) for the co-funding of this publication in Open Access format.Peer reviewedMultidisciplinary Digital Publishing InstituteConsejo Superior de Investigaciones Científicas (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201420142014info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/102893reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.3390/md12031390Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1028932026-05-22T06:33:51Z
dc.title.none.fl_str_mv Identification of the major ACE-inhibitory peptides produced by Eenzymatic hydrolysis of a protein concentrate from cuttlefish wastewater
title Identification of the major ACE-inhibitory peptides produced by Eenzymatic hydrolysis of a protein concentrate from cuttlefish wastewater
spellingShingle Identification of the major ACE-inhibitory peptides produced by Eenzymatic hydrolysis of a protein concentrate from cuttlefish wastewater
Rodríguez-Amado, I.
Ultrafiltration
Proteolysis
ACE inhibitory peptides
Cuttlefish byproducts
Peptide identification
HPLC-ESI-MS
title_short Identification of the major ACE-inhibitory peptides produced by Eenzymatic hydrolysis of a protein concentrate from cuttlefish wastewater
title_full Identification of the major ACE-inhibitory peptides produced by Eenzymatic hydrolysis of a protein concentrate from cuttlefish wastewater
title_fullStr Identification of the major ACE-inhibitory peptides produced by Eenzymatic hydrolysis of a protein concentrate from cuttlefish wastewater
title_full_unstemmed Identification of the major ACE-inhibitory peptides produced by Eenzymatic hydrolysis of a protein concentrate from cuttlefish wastewater
title_sort Identification of the major ACE-inhibitory peptides produced by Eenzymatic hydrolysis of a protein concentrate from cuttlefish wastewater
dc.creator.none.fl_str_mv Rodríguez-Amado, I.
Vázquez, José Antonio
González Fernández, Pilar
Esteban-Fernández, Diego
Carrera, Mónica
Piñeiro, Carmen
author Rodríguez-Amado, I.
author_facet Rodríguez-Amado, I.
Vázquez, José Antonio
González Fernández, Pilar
Esteban-Fernández, Diego
Carrera, Mónica
Piñeiro, Carmen
author_role author
author2 Vázquez, José Antonio
González Fernández, Pilar
Esteban-Fernández, Diego
Carrera, Mónica
Piñeiro, Carmen
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Ultrafiltration
Proteolysis
ACE inhibitory peptides
Cuttlefish byproducts
Peptide identification
HPLC-ESI-MS
topic Ultrafiltration
Proteolysis
ACE inhibitory peptides
Cuttlefish byproducts
Peptide identification
HPLC-ESI-MS
description 16 páginas, 2 tablas, 4 figuras.-- This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/)
publishDate 2014
dc.date.none.fl_str_mv 2014
2014
2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/102893
url http://hdl.handle.net/10261/102893
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.3390/md12031390
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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